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SENP3 mediates the activation of the Wnt/β-catenin signaling pathway to accelerate the growth and metastasis of oesophagal squamous cell carcinoma in mice

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Abstract

As a common malignant tumor, esophageal squamous cell carcinoma (ESCC) is occasionally seen in clinical practice. This type of disease has low incidence rate and mortality. The post-translational modification of small ubiquitin like modifiers (SUMO) can play a crucial role in regulating protein function, and can significantly impact the occurrence and development of diseases. SUMO-specific peptidase (SENP) affects cell activity by regulating the biological function of SUMO. SENP3 belongs to the SENP family, and available data indicate that many malignancies are associated with SENPs, it is currently unclear its role in ESCC. This study indicates that there is a high level of SENP3 expression in ESCC tumor cells. If the expression level of this gene is high, it can have a significant impact on ESCC cell lines and affect physiological activities such as invasion of KYSE170 cells. If the gene is knocked out, this situation will not occur. There is also research data indicating that this gene can effectively activate related signaling pathways, thereby promoting the physiological activities of malignant tumor cells. In a nude mouse xenograft tumor model, KYSE170 cells with SENP3 expression knockdown induced a smaller volume and weight of tumor tissue. Therefore, it can be clearly stated that SENP3 can enable Wnt/ β- The catenin signaling pathway is stimulated, which in turn affects the physiological activities of ESCC cells, including the invasion process. The results of this article lay the foundation for clinical staff to carry out clinical management.

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Data availability

The datasets used during the present study are available from the corresponding author upon reasonable request.

Abbreviations

IGF:

Insulin growth factor

TGF-β:

Transforming growth factor-β

SENP3:

SUMO specific peptidase 3

EGF:

Epidermal growth factor

GSK3β:

Glycogen synthase kinase-3β

SUMO:

Small ubiquitin-like modifier

ESCC:

Esophageal squamous cell carcinoma

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Funding

This work was supported by grants from the Central Government Guided Local Science and Technology Development Fund Project (6010201-Science and Technology Innovation Base Project).

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Authors and Affiliations

  1. Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University, No.12 Jian-Kang Road, Chang-An District, Shijiazhuang, 050011, People’s Republic of China

    Pengzeng Wang & Ziqiang Tian

  2. Department of Respiratory Medicine, the Second Hospital of Hebei Medical University, Shijiazhuang, 050011, People’s Republic of China

    Linan Yang

  3. Department of Radiotherapy, the Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, People’s Republic of China

    Yin Guo

  4. Department of Thoracic Surgery, Gucheng County Hospital in Hebei Province, Hengshui, 253800, People’s Republic of China

    Shuliang Qi

  5. Department of Cancer, the Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, People’s Republic of China

    Jia Liang

  6. Medical Record Room, the Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, People’s Republic of China

    Guo Tian

Authors
  1. Pengzeng Wang
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  2. Linan Yang
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  3. Yin Guo
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  7. Ziqiang Tian
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Contributions

Pengzeng Wang, Yin Guo, and Jia Liang were responsible for the research design. Linan Yang, Shuliang Qi, and Guo Tian conducted the experiments. Pengzeng Wang and Linan Yang were responsible for data acquisition. Shuliang Qi, Jia Liang, and Guo Tian were responsible for data analysis. Pengzeng Wang and Ziqiang Tian were responsible for writing the manuscript. All the authors have contributed to the completion of this paper.

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Correspondence to Ziqiang Tian.

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The authors declare no competing interests.

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The authors declare that no conflicts of interest exist.

Ethical approval

All experiments were conducted according to the Animal Ethics Committee of Hebei Medical University.

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Pengzeng Wang and Linan Yang are co-first authors.

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Wang, P., Yang, L., Guo, Y. et al. SENP3 mediates the activation of the Wnt/β-catenin signaling pathway to accelerate the growth and metastasis of oesophagal squamous cell carcinoma in mice. Funct Integr Genomics 24, 40 (2024). https://doi.org/10.1007/s10142-024-01321-2

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  • DOI: https://doi.org/10.1007/s10142-024-01321-2

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