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Control of Fusarium wilt disease of tomato and improvement of some growth factors through green synthesized zinc oxide nanoparticles

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Abstract

Fusarium wilt of tomato is a destructive disease worldwide, causing economic losses every year. In this research, a biological method was used to control the disease. Metabolite solution of Trichoderma harzianum was used for green synthesis of zinc oxide nanoparticles (ZnO-NPs) from zinc nitrate (ZnNO3), and GC/MS analysis of metabolite was performed. Then, the antifungal activity of the synthesized ZnO-NPs was evaluated in vitro and in vivo conditions. Results were compared to different concentrations of Iprodione+Carbendazim (Rovral-TS®) fungicide. Synthesized ZnO-NPs were characterized using ultraviolet-visible spectrometry (UV-Vis), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR). UV-Vis spectra showed an intense peak at 339 nm. X-ray diffraction pattern showed the crystalline nature of the ZnO-NPs. FTIR revealed various functional groups including phenols, ketones, aldehydes, aliphatic and primary amines, nitriles, alkanes and alkynes in synthesized ZnO-NPs. The size of the ZnO-NPs was determined to be in the range of 25–60 nm. Based on atomic absorption spectroscopy, the foliar application of synthesized ZnO-NPs led to acceptable level of zinc concentration in the leaves and it can be useful to compensate zinc deficiency. Some growth factors showed relative improvement compared to the control and some of them were not significantly different. Complete inhibition of mycelia growth of F. oxysporum was observed in 100 µg/ml concentration of ZnO-NPs in vitro conditions and disease severity was significantly reduced in vivo conditions, indicating that green synthesized ZnO-NPs gave better results in low concentration than the fungicide.

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References

  • Akintelu, S. A., & Folorunso, A. S. (2020). A review on green synthesis of zinc oxide nanoparticles using plant extracts and its biomedical applications. BioNanoScience, 10(4), 848–863.

    Article  Google Scholar 

  • Al-Dhabi, N. A., & Valan Arasu, M. (2018). Environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties. Nanomaterials, 8(7), 500.

    Article  PubMed  PubMed Central  Google Scholar 

  • Amini, J., & Sidovich, D.F. (2010). The effects of fungicides on Fusarium oxysporum f. sp. lycopersici associated with Fusarium wilt of tomato. Journal of Plant Protection Research, 50(2), 172–178.

  • Boruah, S., & Dutta, P. (2021). Fungus mediated biogenic synthesis and characterization of chitosan nanoparticles and its combine effect with Trichoderma asperellum against Fusarium oxysporum, Sclerotium rolfsii and Rhizoctonia solani. Indian Phytopathology, 74, 81–93.

    Article  Google Scholar 

  • Chikh-Rouhou, H., González-Torres, R., & Alvarez, J.M. (2010). Screening and morphological characterization of melons for resistance to Fusarium oxysporum f.sp. melonis Race 1.2. Horticultural Sciences, 45(7), 1021–1025.

  • Elamawi, R. M., Al-Harbi, R. E., & Hendi, A. A. (2018). Biosynthesis and characterization of Silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic Fungi. Egyptian Journal of Biological Pest Control, 28, 28.

    Article  Google Scholar 

  • Fernando, S., Gunasekara, T., & Holton, J. (2018). Antimicrobial nanoparticles: Applications and mechanisms of action. Sri Lankan Journal of Infectious Diseases. https://doi.org/10.4038/sljid.v8i1.8167

    Article  Google Scholar 

  • Gao, Y., Anand, M., Ramachandran, V., Karthikkumar, V., Shalini, V., Vijayalakshmi, S., & Ernest, D. (2019). Biofabrication of zinc oxide nanoparticles from Aspergillus niger, their antioxidant, antimicrobial and anticancer activity. Journal of Cluster Science, 30, 937–946.

    Article  CAS  Google Scholar 

  • Guilger, M., Pasquoto-Stigliani, T., Bilesky-Jose, N., Grillo, R., Abhilash, P. C., Fraceto, L. F., & De Lima, R. (2017). Biogenic silver nanoparticles based on Trichoderma harzianum: Synthesis, characterization, toxicity evaluation and biological activity. Scientific Reports, 7, 44421.

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  • He, L., Liu, Y., Mustapha, A., & Lin, M. (2011). Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiology Research, 166, 207–215.

    Article  CAS  Google Scholar 

  • Ifijen, I. H., Maliki, M., & Anegbe, B. (2022). Synthesis, photocatalytic degradation and antibacterial properties of Selenium or Silver doped Zinc oxide nanoparticles: A detailed review. OpenNano, 8(9), 100082. https://doi.org/10.1016/j.onano.2022.100082

  • Irandegani, Y., Pirnia, M., Taheri, A., Khaledi, N., Keykhasaber, M., & Sarani, S. (2023). Phylogenetic analyses of Fusarium oxysporum species complex on Banana in Iran and evaluation of some essential oils on the growth, sporulation and spore germination of the fungus. European Journal of Plant Pathology, 166(2). https://doi.org/10.1007/s10658-023-02691-2.

  • Iranmanesh, S., Aran, M. Miri, M.A., & Pirnia, M. (2022). Preparation and characterization of zein electrospun fibers for nano encapsulation of Ajowan essential oil. Journal of Essential Oil Bearing Plants, 25. https://doi.org/10.1080/0972060X.2022.2068970.

  • Jitao, L. V., Zhang, S., Luo, L., Zhang, J., Yang, K., & Christied, P. (2015). Accumulation, speciation and uptake pathway of ZnO nanoparticles in maize. Environmental Science: Nano, 2, 68.

    Google Scholar 

  • Khan, M., & Siddiqui, Z. A. (2018). Zinc oxide nanoparticles for the management of Ralstonia solanacearum, Phomopsis vexans and Meloidogyne incognita incited disease complex of eggplant. Indian Phytopathology, 71(3), 355–364.

    Article  Google Scholar 

  • Mosa, M.A., & Youssef, K. (2021). Topical delivery of host induced RNAi silencing by layered double hydroxide nanosheets: An efficient tool to decipher pathogenicity gene function of Fusarium crown and root rot in tomato. Physiological and Molecular Plant Pathology, 101684. https://doi.org/10.1016/j.pmpp.2021.101684.

  • Nandiyanto, A. B. D., Oktiani, R., & Ragadhita, R. (2019). How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology, 4, 97.

    Article  Google Scholar 

  • Ogunyemi, S. O., Abdallah, Y., Zhang, M., Fouad, H., Hong, X., Ibrahim, E., & Li, B. (2019). Green synthesis of zinc oxide nanoparticles using dierent plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae. Artificial Cells, Nanomedicine and Biotechnology, 47, 341–352.

    Article  CAS  PubMed  Google Scholar 

  • Perveen, R., Shujaat, S., Qureshi, Z., Nawaz, S., Khan, M. I., & Iqbal, M. (2020). Green versus sol-gel synthesis of ZnO nanoparticles and antimicrobial activity evaluation against panel of pathogens. Journal of Materials Research and Technology, 9, 7817–7827.

    Article  CAS  Google Scholar 

  • Rajan, A., Cherian, E., & Baskar, G. (2016). Biosynthesis of zinc oxide nanoparticles using Aspergillus fumigatus JCF and its antibacterial activity. International Journal of Modern Science and Technology, 1, 52–57.

    Google Scholar 

  • Rasteh, I., Pirnia, M., Miri, M.A., & Sarani, S. (2024). Encapsulation of Zataria multiflora essential oil in electrosprayed zein microcapsules: Characterization and antimicrobial properties. Industrial Crops & Products, 208. https://doi.org/10.1016/j.indcrop.2023.117794.

  • Shelar, G. B., & Chavan, A. M. (2015). Myco-synthesis of silver nanoparticles from Trichoderma harzianum and its impact on germination status of oil seed. Biolife, 3, 109–113.

    Google Scholar 

  • Shobha, B., Lakshmeesha, T.R., Ansari, M.A., Almatroudi, A., Alzohairy, M.A., Basavaraju, S., Alurappa, R., Niranjana, S.R., & Chowdappa, S. (2020). Mycosynthesis of ZnO nanoparticles using Trichoderma spp. isolated from rhizosphere soils and its synergistic antibacterial Effect against Xanthomonas oryzae pv. oryzae. Journal of Fungi, 6, 181.

  • Siddiquee, S., Cheong, B. E., Taslima, K., Kausar, H., & Hasan, M. M. (2012). Separation and identification of volatile compounds from liquid cultures of Trichoderma harzianum by GC-MS using three dierent capillary columns. Journal of Chromatographic Science, 50, 358–367.

    Article  CAS  PubMed  Google Scholar 

  • Singh, J., Kumar, S., Alok, A., Upadhyay, S. K., Rawat, M., Tsang, D. C., Bolan, N., & Kim, K. H. (2019). The potential of green synthesized zinc oxide nanoparticles as nutrient source for plant growth. Journal of Cleaner Production, 214, 1061–1070.

    Article  CAS  Google Scholar 

  • Teixeira, G. M., Mosela, M., Nicoletto, M. L. A., Ribeiro, R. A., Hungria, M., Youssef, K., Higashi, A. Y., Mian, S., Ferreira, A. S., Gonçalves, L. S. A., de Padua Pereira, U., & de Oliveira, A. G. (2021). Genomic insights into the antifungal activity and plant growth-promoting ability in Bacillus velezensis CMRP 4490. Frontiers in Microbiology, 11, 618415. https://doi.org/10.3389/fmicb.2020.618415

    Article  PubMed  PubMed Central  Google Scholar 

  • Yehia, R. S., & Ahmed, O. F. (2013). In vitro study of the antifungal efficacy of zinc oxide nanoparticles against Fusarium oxysporum and Penicilium expansum. African Journal of Microbiology Research, 7, 1917–1923.

    Article  CAS  Google Scholar 

  • Zaki, S. A., Ouf, S. A., Albarakaty, F. M., Habeb, M. M., Aly, A. A., & Abd-Elsalam, K. A. (2021). Trichoderma harzianum-mediated ZnO nanoparticles: A green tool for controlling soil-borne pathogens in Cotton. Journal of Fungi, 7, 952. https://doi.org/10.3390/jof7110952

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was funded by University of Zabol, Grant number: IR-UOZ-GR-7062. The authors would like to thank the Research Deputy of University of Zabol for financial support.

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University of Zabol,IR-UOZ-GR-7062,Mahdi Pirnia

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Authors and Affiliations

  1. Department of Plant Protection, University of Zabol, Zabol, Iran

    Aminsajad Jomeyazdian, Mahdi Pirnia & Shirahmad Sarani

  2. Department of Plant Protection, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

    Hossein Alaei

  3. Department of Plant Production, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran

    Abdolhosein Taheri

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  1. Aminsajad Jomeyazdian
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  2. Mahdi Pirnia
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  3. Hossein Alaei
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  4. Abdolhosein Taheri
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  5. Shirahmad Sarani
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Correspondence to Mahdi Pirnia.

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This research focused on green synthesis of ZnO-NPs and their antifungal effect on Fusarium wilt disease of tomato.

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Jomeyazdian, A., Pirnia, M., Alaei, H. et al. Control of Fusarium wilt disease of tomato and improvement of some growth factors through green synthesized zinc oxide nanoparticles. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02831-2

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