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Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honey

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Abstract

A fluorescence polarization immunoassay method has been developed for the determination of antibiotic tylosin in honey. For this purpose, rabbits were immunized with a conjugate of tylosin and bovine serum albumin, and antisera were obtained. The conditions for the interaction of antisera in a competitive format with tylosin in the sample and with tylosin labeled with fluorescein were optimized. The developed method is characterized by a limit of detection for tylosin of 34.7 ng/mL and an analytical range of 65.2 to 564.0 ng/mL. High reproducibility of measurements using a portable detector was demonstrated, with relative standard deviation (RSD) values of 1.5–2.5%. The method was validated using honey samples, showing recovery ranging from 98 to 103.8%. An analysis time, excluding sample preparation, is 10 min.

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Funding

This work was supported by the Russian Science Foundation, project no. 20-76-10033.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    S. A. Eremin, L. I. Mukhametova, D. A. Arutyunyan & N. V. Sumbatyan

  2. Bach Institute of Biochemistry, Research Center “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

    S. A. Eremin, A. N. Berlina & D. V. Sotnikov

  3. Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, 119234, Moscow, Russia

    A. G. Tereshchenkov

  4. All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed, 123022, Moscow, Russia

    A. D. Priima & I. S. Nesterenko

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  1. S. A. Eremin
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Correspondence to S. A. Eremin.

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Translated by O. Zhukova

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Eremin, S.A., Mukhametova, L.I., Arutyunyan, D.A. et al. Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honey. J Anal Chem 79, 154–161 (2024). https://doi.org/10.1134/S1061934824020096

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  • DOI: https://doi.org/10.1134/S1061934824020096

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