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Acute hypoxia causes glycolysis and lipid metabolism alterations with HSPs’ persistent activation in Leiocassis longirostris

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Abstract

Hypoxia is one of the common stress factors encountered in intensive farming. Leiocassis longirostris is a commercially valuable fish with limited information on the response mechanism under acute hypoxia stress. Here, we detected the asphyxiation point and simulated acute hypoxia conditions, dissolved oxygen decreased, hypoxia maintained, and reoxygenation, and investigated molecular changes in the liver of L. longirostris. Results showed that the hypoxia-inducible factor-1 signaling pathway was enriched and hif1α, arnt, hph, epo, epor, and glut1 gene expression significantly increased. Peroxisome proliferator-activated receptor pathway with lipid metabolism-related genes (gk, scd, acsl, pgar, lpl, fabp3) was inhibited, while the glycolysis pathway with its related genes (pk, gapdhs, ldh, pfk, aldo, hk, gpi, pfkfb3) was activated under hypoxia. Furthermore, heat shock protein family gene expression increased significantly in both hypoxia treatments and the reoxygenation group, which suggests that immune response persistent activation was induced by acute hypoxic stress. This study provided valuable information for the metabolism and immune response in L. longirostris liver under acute hypoxia and reoxygenation conditions.

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Acknowledgments

This research was supported by the National Key Research and Development Program of China (2022YFD2400903), Chongqing Alliance for Aquatic Science and Technology Innovation (CQFTIU2022-09), and Shuangcheng cooperative agreement research grant of Yinbin, China (XNDX20220004).

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Authors and Affiliations

  1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), College of Fisheries, Southwest University, Chongqing, 402460, China

    Mengdan Hou, Zhenlin Ke, Mengbin Xiang, Yuejing Yang, Zhe Li, Wenbo Sun, Zhengshi Zhang, Hua Ye & Hui Luo

  2. Fisheries Research Institute in Wanzhou Chongqing, Chongqing, 404000, China

    Mengbin Xiang

  3. Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 611731, Sichuan, China

    Lu Zhang, Yuejing Yang & Jian Zhou

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  1. Mengdan Hou
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  2. Zhenlin Ke
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  4. Lu Zhang
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  9. Jian Zhou
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  10. Hua Ye
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  11. Hui Luo
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Correspondence to Hua Ye or Hui Luo.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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This study has been approved by the Committee of Laboratory Animal Experimentation at Southwest University (xdjk2017b008) and followed the legal requirements or guidelines in China for the care and use of animals.

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Hou, M., Ke, Z., Xiang, M. et al. Acute hypoxia causes glycolysis and lipid metabolism alterations with HSPs’ persistent activation in Leiocassis longirostris. Ichthyol Res (2024). https://doi.org/10.1007/s10228-024-00954-4

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  • DOI: https://doi.org/10.1007/s10228-024-00954-4

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