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Knockdown of ANGPTL4 inhibits adipogenesis of preadipocyte via autophagy

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Abstract

It has been demonstrated that angiopoietin-like protein 4 (ANGPTL4) plays an important regulatory role in lipid metabolism and backfat deposition appears to vary in different pig breeds. However, the correlation between ANGPTL4 and backfat deposition have not been well characterized and the role of ANGPTL4 in regulating adipogenesis remains unclear. Therefore, this study aimed to investigate correlation between ANGPTL4 and backfat deposition and to explore the effects of ANGPTL4 on preadipocyte differentiation and the underlying mechanism. Our results showed that the backfat thickness and the ANGPTL4 gene expression of Laiwu pigs were significantly higher than those in DLY pigs and the ANGPTL4 gene expression was positively correlated with backfat thickness both in DLY pigs and Laiwu pigs. Moreover, an increase in ANGPTL4 expression and activation of autophagy were observed during the differentiation of stromal vascular fraction cells. In addition, knockdown of ANGPTL4 inhibited the differentiation of 3T3-L1 cells with decreased expression of LC3-II and ATG5 and increased expression of SQSTM1, suggesting the involvement of autophagy in ANGPTL4-mediated adipogenesis. In conclusion, these results suggested that ANGPTL4 is positively correlated with backfat deposition in pigs and knockdown of ANGPTL4 inhibits adipogenesis of preadipocyte via autophagy, providing new insights into the regulation of fat deposition and to improve the carcass quality and meat quality of porcine.

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Data Availability

All important data is included in the manuscript and raw data are available upon request.

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Funding

The present study was supported by the earmarked fund for China Agriculture Research System (CARS-18).

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Authors and Affiliations

  1. Jiangsu Key laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China

    Mingfa Ling, Heying Qian & Huiduo Guo

  2. Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agricultural and Rural Affairs, The Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212100, China

    Heying Qian

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  1. Mingfa Ling
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  2. Heying Qian
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Contributions

ML: performed the experiment, analyzed the data and writing—original draft and validation. HQ: writing—review and editing. HG: supervision and project administration. All authors read and approved the final manuscript.

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Correspondence to Huiduo Guo.

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Ethical approval

The study was conducted in accordance with requirements of Jiangsu University of Science and Technology (G2022SJ12, Zhenjiang, China) and approved by the Ethics Committee.

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The authors declare no competing interests.

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Ling, M., Qian, H. & Guo, H. Knockdown of ANGPTL4 inhibits adipogenesis of preadipocyte via autophagy. In Vitro Cell.Dev.Biol.-Animal 60, 258–265 (2024). https://doi.org/10.1007/s11626-024-00861-y

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  • DOI: https://doi.org/10.1007/s11626-024-00861-y

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