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Antimicrobial Susceptibility Patterns of Enterococcus Species and Molecular Detection of Enterococcus faecalis Isolated from Patients with Urinary Tract Infection in a Tertiary Care Hospital in Bangladesh

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Abstract

One of the most prevalent infectious diseases identified in both communities and hospitalized patients is urinary tract infection (UTI). Enterococcus is evolved into a clinically pertinent uropathogen due to its evolving resistance to multiple antimicrobial agents.This study, detects antimicrobial susceptibility patterns of Enterococcus species and molecular detection of Enterococcus faecalis from patients with urinary tract infections. In this cross-sectional observational study, 165 urine samples were obtained from clinically diagnosed patients with UTIs of different ages and gender. Enterococcus species were identified by standard microbiological procedure and PCR (by using species-specific primers for Enterococcus faecalis). A modified Kirby Bauer Disc diffusion method was used to identify the antimicrobial susceptibility pattern following Clinical and Laboratory Standards Institute (CLSI) guidelines. Out of 165 urine samples, 134 samples yielded positive cultures. Enterococcus species were isolated from 23 (17.1%) urine samples. Among all Enterococcus, 16 (69.6%) isolates were E. faecalis, detected by PCR assay. A higher (30.4%) proportion of Enterococcus-positive patients were from the age group 48–57 years and female patients (78.2%) had a higher prevalence. Enterococcal infection was found in 56.5% of non-catheterized patients and 43.5% of catheterized patients. Vancomycin and linezolid (78.3%) and meropenem (73.9%) sensitivity was prevalent among all Enterococcus species. They showed 100% resistance towards ceftriaxone, cefixime 95.7%, cefuroxime 91.3%, azithromycin 82.6%. This research indicated the occurrence of Enterococcus species and the advent of multidrug-resistant E. faecalis in patients with UTIs. Routine speciation and antimicrobial susceptibility testing of Enterococcus in various clinical samples is encouraged.

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Data Availability

The datasets generated during and/or analysed during the current study are not publicly available due to these are classified data concerning patients confidential infoemations but are available from the corresponding author on reasonable request.

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Funding

The authors did not receive support from any organization for the submitted work.

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Authors and Affiliations

  1. Department of Microbiology, MARKS Medical College and Hospital, Dhaka, Bangladesh

    Nahla Islam Neeva

  2. Department of Medicine, Sylhet MAG Osmani Medical College, Sylhet, Bangladesh

    Nahida Zafrin

  3. Department of Microbiology and Virology, Sylhet MAG Osmani Medical College, Sylhet, Bangladesh

    Azima Aktar Jhuma

  4. Department of Microbiology, Parkview Medical College, Sylhet, Bangladesh

    Sufia Khanom Chowdhury

  5. Department of Microbiology, Cox’s Bazar Medical College, Cox’s Bazar, Bangladesh

    Kaniz Fatema

  6. Department of Community Medicine, Sylhet Women’s Medical College, Sylhet, Bangladesh

    Tafhim Ahmed Rifat

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, methodology and analysis were performed by [Nahla Islam Neeva]. The manuscript was written by [Nahla Islam Neeva] [Nahida Zafrin] and review and editing by [Nahida Zafrin]. Supervised by [Nahida Zafrin] [Azima Aktar Jhuma]. All authors read and approved the final manuscript.

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Correspondence to Nahla Islam Neeva.

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The authors have no relevant financial or non-financial interests to disclose.

Ethical Approval

The above research project has been granted Ethical permission based research guide’s forwarding and reviewers subsequent favourable observation and comments. Approval was granted by the Ethical Committee and Principal of Sylhet MAG Osmani Medical College (Date 29.05.2021/No/SOMC/2021/33).

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Neeva, N.I., Zafrin, N., Jhuma, A.A. et al. Antimicrobial Susceptibility Patterns of Enterococcus Species and Molecular Detection of Enterococcus faecalis Isolated from Patients with Urinary Tract Infection in a Tertiary Care Hospital in Bangladesh. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-024-01216-7

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