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Evaluation of the Stability of Regenerant Oat Lines Developed from In Vitro Callus Cultures

  • AGRICULTURE AND CROP PRODUCTION
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The study was carried out to compare the productivity and stability of regenerant lines of spring oat and their donor husky (Tubinsky st. (3 lines), Sayan (3), and Kazyr (1)) and naked (Tyumensky golozerny (3 lines) and Golets (2)) varieties. Field tests were carried out in the forest-steppe zone of the Krasnoyarsk krai on leached chernozem. Criteria used for the line stability evaluation were the following: superiority index Pi determining the degree of a sample deviation from the maximum yield in each of the environments (years); S(1) index characterizing the predictability of a genotype response to growing conditions; S(3) index, aimed at identifying highly productive and highly stable genotypes; and NP(2) index, intended for selection of genotypes with the optimal productivity/stability ratio. The majority of regenerants showed the yield and 1000 kernel weight parameters to be close to the donor varieties. Only three lines of the Tubinsky variety surpassed the donor in terms of the average three-year productivity by 1.78 (SR6-Tub.), 2.45 (SR11-Tub.), and 2.95 (SR15-Tub.) t/ha. Note that the 1000-kernel weight in the SR6-Tub. and ZR4-Golets lines was lower than that of the original variety by 1.9 and 1.4 g, respectively. A 1000 kernel weight in the ZR15-Kazyr line in 2017 was inferior to that in the donor variety by 1.4 g. In terms of the yield stability indices Pi and S(1), the ranks of nine out of 12 studied lines exceeded that of initial donor varieties by 5.7 on average. The similar result was observed in four (Pi higher by 2.25 rank units) and eight (S(1) higher by 6.6 rank units) regenerant lines. In relation to the S(3) index, six out of twelve lines surpassed their donors by 7 rank units on average; in terms of the 1000 kernel weight, nine lines exceeded their donors by 5.3 rank units. The rank determined for the NP(2) index exceeded that of donor varieties by 5.5 in four lines in terms of yield and by 6.2 in ten lines in terms of a 1000 kernel weight.

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Funding

The study was carried out within the framework of the State Assignment of the Ministry of Science and Higher Education of Russian Federation (theme no. FWES-2024-0030.

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Authors and Affiliations

  1. Krasnoyarsk Research Institute of Agriculture, Federal Research Center, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, 660041, Krasnoyarsk, Russia

    V. Yu. Stupko & S. Yu. Lugovtsova

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  1. V. Yu. Stupko
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  2. S. Yu. Lugovtsova
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Correspondence to V. Yu. Stupko.

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Translated by N. Statsyuk

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Stupko, V.Y., Lugovtsova, S.Y. Evaluation of the Stability of Regenerant Oat Lines Developed from In Vitro Callus Cultures. Russ. Agricult. Sci. 49 (Suppl 2), S257–S264 (2023). https://doi.org/10.3103/S1068367423080189

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  • DOI: https://doi.org/10.3103/S1068367423080189

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