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Microsatellite Analysis of the Influence of Sebastes schlegelii Restocking on the Genetic Structure of Stocks in Rongcheng Bay

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Abstract

The black rockfish Sebastes schlegelii, a valuable commercial fish, is one of the most important fish species for marine fishery stock enhancement in China. In this study, genetic diversity of the hatchery-released stock and two restored-natural stocks (SSNAC1 and SSNAC2) of S. schlegelii from Rongcheng Bay, Yellow Sea, China was analyzed using 15 microsatellite DNA loci, and the genetic differentiation between natural and hatchery-released stocks was also investigated. The mean effective number of alleles (Ae) in the hatchery-released stock was 3.6, the observed heterozygosity (Ho) was 0.506, and the expected heterozygosity (He) was 0.606, while the Ae, Ho and He values in the SSNAC1 and SSNAC2 stocks were 2.9 and 3.9, 0.381 and 0.577, and 0.569 and 0.692, respectively. The FST values between the hatchery-released and natural stock and the natural stocks before and after restocking were 0.055 and 0.013, respectively. The results showed that there was low differentiation between the natural and hatchery-released stocks even though the hatchery stock displayed slightly higher genetic diversity and heterozygosity than the natural stock. Furthermore, there was no differentiation on genetic diversity indices between the S. schlegelii natural stock before release and the mixed stocks after release in the short term. However, larger differentiation was found between the natural stock and restored-natural stock SSNAC2. Consequently, more detailed surveys are required to elucidate the long-term genetic effects of the stock enhancement. The present study would be helpful for the future stock enhancement strategy of black rockfish.

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Acknowledgements

This research was funded by the Key Research and Development Programs of Shandong Province (No. 2021LZGC029), the National Special Research Fund for Non-Profit Marine Sector (No. 201305043 and 200805069), and the National Marine Genetic Resource Center.

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  1. Yi Zhang and Lijuan Wang contributed equally to this work.

Authors and Affiliations

  1. CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Yi Zhang, Lijuan Wang, Zhihao Wu & Feng You

  2. Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China

    Yi Zhang, Lijuan Wang, Zhihao Wu & Feng You

  3. Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao), Qingdao, 266104, China

    Hongjun Liu

  4. Weihai Shenghang Aquatic Science and Technology Co., Ltd, Weihai, 264200, China

    Zongcheng Song

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Zhang, Y., Wang, L., Wu, Z. et al. Microsatellite Analysis of the Influence of Sebastes schlegelii Restocking on the Genetic Structure of Stocks in Rongcheng Bay. Thalassas (2024). https://doi.org/10.1007/s41208-024-00688-0

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