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MicroRNA-34b-5p increases chemosensitivity of hepatocellular carcinoma cells

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Molecular & Cellular Toxicology Aims and scope Submit manuscript

Abstract

Objective

Increasing evidence shows that microRNA (miR) is related to drug resistance in hepatocellular carcinoma (HCC). miR-34b-5p could retard tumor development, but its function in HCC is still unclear.

Methods

miR-34b-5p expression was determined in HCC tissues and cell lines. Analysis and verification of the binding between miR-34b-5p and metadherin (MTDH) was carried out. The actual action of miR-34b-5p and MTDH in the area of proliferation, apoptosis, invasion, and migration of cisplatin (DDP)-resistant HCC cells was monitored.

Results

miR-34b-5p was downregulated in HCC. Overexpression of miR-34b-5p enhanced the sensitivity of HCC cells to DDP, inhibiting proliferation, migration, and invasion and promoting apoptosis. MTDH was the direct target of miR-34b-5p. MTDH was upregulated in HCC tissues, which was negatively correlated with miR-34b-5p expression. Enhancement of MTDH can reverse the effect of upregulated miR-34b-5p on the chemosensitivity of HCC cells.

Conclusion

miR-34b-5p targets MTDH and enhances the chemosensitivity of HCC cells.

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Data availability

Data are available from the corresponding author on request.

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Acknowledgements

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Author notes

  1. JinSuo Chen and LiNa Wang have contributed equally to this work.

Authors and Affiliations

  1. Department of Hepatobiliary and Pancreatic Surgery, Jiangnan University Medical Center (Wuxi No. 2 People’s Hospital), Wuxi, 214002, Jiangsu, China

    JinSuo Chen, WenXi Cao, LeiLei Yang, YongPing Zhou & Li Xia

  2. Department of Critical Care Medicine, Yantai Qishan Hospital, Yantai, 264001, Shandong, China

    LiNa Wang

  3. Department of Outpatient, The General Hospital of Western Theater Command PLA, Chengdu, 610000, Sichuan, China

    XueMei Wu

  4. Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Wuxi No. 2 People’s Hospital of Nanjing Medical University, Wuxi, 214003, Jiangsu, China

    ZhiJie Ding

  5. Department of Hepatobiliary and Pancreatic Surgery, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan, 250013, Shandong, China

    Zhao Song

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  1. JinSuo Chen
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Contributions

JC and LW designed the research study. XW and ZD performed the research. WC and LY provided help and advice. YZ, LX, and ZS analyzed the data. JC and LW wrote the manuscript. ZS reviewed and edited the manuscript. All authors contributed to editorial changes in the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zhao Song.

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Conflict of interests

JinSuo Chen, LiNa Wang, XueMei Wu, ZhiJie Ding, WenXi Cao, LeiLei Yang, YongPing Zhou, Li Xia, and Zhao Song have no conflicts of interest to declare.

Ethical statement

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All subjects was approved by Jiangnan University Medical Center (Approval number is JN2014-0832).

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Chen, J., Wang, L., Wu, X. et al. MicroRNA-34b-5p increases chemosensitivity of hepatocellular carcinoma cells. Mol. Cell. Toxicol. (2024). https://doi.org/10.1007/s13273-024-00431-z

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