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Association of a Skin Dressing Made With the Organic Part of Marine Sponges and Photobiomodulation on the Wound Healing in an Animal Model

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Abstract

The present study aims to characterize and to evaluate the biological effects of a skin dressing manufactured with the organic part of the Chondrilla caribensis marine sponge (called spongin-like collagen (SC)) associated or not to photobiomodulation (PBM) on the skin wound healing of rats. Skin dressings were manufactured with SC and it was characterized using scanning electron microscopy (SEM) and a tensile assay. In order to evaluate its biological effects, an experimental model of cutaneous wounds was surgically performed. Eighteen rats were randomly distributed into three experimental groups: control group (CG): animals with skin wounds but without any treatment; marine collagen dressing group (DG): animals with skin wounds treated with marine collagen dressing; and the marine collagen dressing + PBM group (DPG): animals with skin wounds treated with marine collagen dressing and PBM. Histopathological, histomorphometric, and immunohistochemical evaluations (qualitative and semiquantitative) of COX2, TGFβ, FGF, and VEGF were done. SEM demonstrates that the marine collagen dressing presented pores and interconnected fibers and adequate mechanical strength. Furthermore, in the microscopic analysis, an incomplete reepithelialization and the presence of granulation tissue with inflammatory infiltrate were observed in all experimental groups. In addition, foreign body was identified in the DG and DPG. COX2, TGFβ, FGF, and VEGF immunostaining was observed predominantly in the wound area of all experimental groups, with a statistically significant difference for FGF immunostaining score of DPG in relation to CG. The marine collagen dressing presented adequate physical characteristics and its association with PBM presented favorable biological effects to the skin repair process.

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Data Availability

The data sets generated during the current study are available from the corresponding author on request.

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Funding

No funding was received for conducting this study.

Author information

Authors and Affiliations

  1. Department of Biosciences, Federal University of São Paulo (UNIFESP), Lab 342, Rua Silva Jardim 136, Vila Matias, 136 Silva Jardim Street, Santos, SP, 11015020, Brazil

    Abdias Fernando Simon Sales, Karolyne dos Santos Jorge Sousa, Amanda de Souza, Mirian Bonifacio, Tiago Akira Tashiro Araújo, Matheus de Almeida Cruz, Márcia Busanello Costa, Daniel Araki Ribeiro, Cintia Cristina Santi Martignago & Ana Cláudia Rennó

  2. Scientific Institute and Technological Department, University Brazil, São Paulo-Itaquera, SP, Brazil

    Lívia Assis

Authors
  1. Abdias Fernando Simon Sales
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  2. Karolyne dos Santos Jorge Sousa
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  3. Amanda de Souza
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  4. Mirian Bonifacio
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  5. Tiago Akira Tashiro Araújo
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  10. Cintia Cristina Santi Martignago
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  11. Ana Cláudia Rennó
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Contributions

The research study was designed by AFSS, ACMR, LAG, CCSM, and TAT. The research was performed by AFSS, LAG, CCSM, MBC, MDAC, TAT, ADS, and KDSJS. The samples for the analysis were prepared by AFSS, LAG, CCSM, and MDAC. The data were analyzed by AFSS, LAG, CCSM, and DAR. The original draft was prepared by AFSS, ACMR, CCSM, and DAR, and MBC, MDAC, ADS, KDSJS, and MB reviewed the paper before its submission.

Corresponding author

Correspondence to Ana Cláudia Rennó.

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Ethics Approval and Consent to Participate

The present study was approved by the Animal Care Committee guidelines at the Federal University of São Paulo (protocol 9645290620) and it was conducted according to the Guiding Principles for the Use of Laboratory Animals.

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The authors declare no competing interests.

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Sales, A.F.S., dos Santos Jorge Sousa, K., de Souza, A. et al. Association of a Skin Dressing Made With the Organic Part of Marine Sponges and Photobiomodulation on the Wound Healing in an Animal Model. Mar Biotechnol 26, 276–287 (2024). https://doi.org/10.1007/s10126-024-10295-y

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