Abstract
Ficus tikoua Bureau is an ecologically functional perennial vine that belongs to the Moraceae family. Tissue culture is a common method for the rapid propagation of plants and is suitable for the rapid propagation of F. tikoua. The objective of the current study was to evaluate the process of F. tikoua organogenesis in vitro utilizing stem explants. An efficient tissue culture propagation method was developed by manipulating several plant growth regulators (PGRs) and their proportions, as well as evaluating alternative medium options for specific phases of differentiation. Explants were cultured on MS medium, supplemented with various thidiazuron (TDZ) concentrations with 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA), to investigate their efficacy during in vitro shoot organogenesis. The results demonstrated that the combination of 0.5 mg L−1 BA, 1.0 mg L−1 NAA, and 1.0 mg L−1 TDZ was the most effective for callus formation (91.1%) and that the combination of 2.0 mg L−1 BA and 0.5 mg L−1 TDZ was the most suited for adventitious shoot induction (61.11%). The medium supplemented with 1.0 mg L−1 BA, 1.0 mg L−1 NAA, and 0.5 mg L−1 TDZ yielded the highest bud multiplication number with a 6.05 proliferation factor. The cultured shoots rooted easily on half-strength MS medium containing 1.0 mg L−1 IBA and 0.1 mg L−1 NAA (68.52%). The subsequent regenerated plants were grown in a mixture of garden soil, perlite, and vermiculite in a ratio of 2:1:1, resulting in a survival rate of 86.17%.
Similar content being viewed by others
Data Availability
All data generated or analyzed during this study are included in this article.
References
Aggarwal G, Gaur A, Srivastava DK (2015) Establishment of high frequency shoot regeneration system in Himalayan poplar (Populus ciliata Wall ex Royle) from petiole explants using thidiazuron cytokinin as plant growth regulator. J Forest Res 26:651–656. https://doi.org/10.1007/s11676-015-0048-6
Babaoglu M, Yorgancilar M (2000) TDZ-specific plant regeneration in salad burnet. Plant Cell Tiss Org Cult 63:31–34. https://doi.org/10.1023/A:1006474002186
Fu GM, Li WJ, Huang XZ, Zhang RZ, Tian K, Hou SQ, Li YK (2018) Antioxidant and alpha-glucosidase inhibitory activities of isoflavonoids from the rhizomes of Ficus tikoua Bur. Nat Prod Res 32:399–405. https://doi.org/10.1080/14786419.2017.1312391
Gong Y, Luo W, Chen H, Ren B, Hu W, Li L (2022) Systematical ingredient investigations of Ficus tikoua Bur. fruit and immunoregulatory and antioxidant effects of different fractions. Molecules. 27:6880. https://doi.org/10.3390/molecules27206880
Hasan M, Ayasha A, Arjina AM, Hasan MMS, Ashraful HM, Monika T, Hasan M (2022) Tissue culture in ornamentals: cultivation factors, propagation techniques, and its application. Plants 11:3208. https://doi.org/10.3390/PLANTS11233208
Heydari H, Chamani E (2022) High‑frequency plant regeneration and genetic fidelity assessment of regenerants by molecular and biochemical markers in woodland sage (Salvia nemorosa L). Ind Crop Prod 187. https://doi.org/10.1016/j.indcrop.2022.115404
Ji W, Deng HS, Yu B, Yan L (2019) High-throughput sequencing uncover Ficus tikoua Bur chloroplast genome. J Plant Biochem Biotechnol 29:171–182
Jiang ZY, Li SY, Li WJ, Guo JM, Tian K, Hu QF, Huang XZ (2013) Phenolic glycosides from Ficus tikoua and their cytotoxic activities. Carbohyd Res 382:19–24
Li C, Yong W, Zhi Y, Mubarak H, Mirza N (2017) Physiological characteristics of Ficus tikoua under antimony stress. Trans Nonferrous Metal Soc China 27:939–945. https://doi.org/10.1016/S1003-6326(17)60106-7
Lin JG, Chen S, Wu SH, Lin SS, Lin XY (2011) Study on tissue culture and rapid propagation technologies of Ficus elastica. Acta Agr Jiangxi 23:55–56
Liu CH, Lun X, Xia GH (2010) Tissue culture and rapid propagation of Ficus pandurata Hance varangustifolia cheng. Plant Physiol Comm 46:603–604. https://doi.org/10.3724/SP.J.1077.2010.01263
Liu J (2014) Study on tissue culture and plantlet regeneration of BoJihong Ficus carica L. Dissertation, Hebei Agricultural University.
Mostafa SK (2018) In vitro propagation of conifers using mature shoots. J Forest Res 29:565–574. https://doi.org/10.1007/s11676-018-0608-7
Munthali C, Kinoshita R, Onishi K, Rakotondrafara A, Mikami K, Koike M, Tani M, Palta J, Aiuchi D (2022) A model nutrition control system in potato tissue culture and its influence on plant elemental composition. Plants 11:2718. https://doi.org/10.3390/PLANTS11202718
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Phillips GC, Garda M (2019) Plant tissue culture media and practices: an overview. In Vitro Cell Dev Biol - Plant 55:242–257
Raisz LG (1965) Bone resorption in tissue culture factors influencing the response to parathyroid PGR. J Clin Invest 44:103
Schween G, Schwenkel HG (2002) In vitro regeneration in Primula ssp. via organogenesis. Plant Cell Rep 20:1006–1010. https://doi.org/10.1007/s00299-002-0443-2
Singh B, Sharma RA (2023) Updated review on Indian Ficus species. Arabian J of Chem 16:104976. https://doi.org/10.1016/J.ARABJC.2023.104976
Su YH, Liu YB, Zhang XS (2011) Auxin cytokinin interaction regulates meristem development. Mol Plant 4:616–625. 10.10931mplssrO07
Velcheva M, Faltin Z, Vardi A, Eshdat Y, Perl A (2005) Regeneration of Aloe arborescens via somatic organogenesis from young inflorescences. Plant Cell Tiss Org Cult 6:293–301
Wang Y (2010) Rooting and hardening-seedling techniques for tissue culture of raspberry. J Northeast Forest Univ 38:121–122. https://doi.org/10.13759/j.cnki.dlxb.2010.06.013
Yang XQ, Xie GF, Wu Q, Chen L, Chen J, Liu YL (2020) Analysis of active compounds, antioxidant activity and correlation in different parts from Ficus tikoua Bur. Food Machine 36:169–174. https://doi.org/10.13652/j.issn.1003-5788.2020.05.032
Yao LL, Zhao SC, Liu W, Wang GZ, Wan CP (2022) Chemical constituents and biological activities of Ficus tikoua Bureau. J Chem 2022:13. https://doi.org/10.1155/2022/2423525
Yu T, Yang B, Zhai SH, Su WH, Zhang GF (2018) Effect of different substrates composition on rooting of cutting of Ficus tikoua. J Kunming Univ 40:80–82
Zhang JH, Su ZH, Zhang ZX, Li MF, Guo SJ, Cao XS, Ji LS (2019) Study on construction of in vitro regeneration culture system of sweet pepper (Capsicum frutescens L.). Mol Plant Breed 17:8208–8214. https://doi.org/10.13271/j.mpb.017.008208
Zhang R, Wang X, Shao L, Shen K, Liu Z, Chen X, Xia Y, Zhang J, Li D (2024) Feasible strategies for efficient propagation system of Paeonia lactiflora ‘Hang Baishao’: in vitro culture of embryos. Sci Hort 326:112726. https://doi.org/10.1016/j.scienta.2023.112726
Zhou Q, Lei X, Niu J, Chen Y, Shen X, Zhang N (2023) A new hemiacetal chromone racemate and α-glucosidase inhibitors from Ficus tikoua Bur. Nat Prod Res 37:3267–3275. https://doi.org/10.1080/14786419.2022.2068544
Zhou SY, Wang R, Deng LQ, Zhang XL, Chen M (2018) A new isoflavanone from Ficus tikoua Bur. Nat Prod Res 21:2516–2522. https://doi.org/10.1080/14786419.2017.1423307
Zhou Y, Sun K, Mi Y, Luo B, Xiang J, Ma T (2022) Insights into the nurse effect of a native plant Ficus tikoua on Pb-Zn tailing wastelands in western Hunan, China. Environ Sci Pollut Res 29:15905–15914. https://doi.org/10.1007/s11356-021-16982-w
Author information
Authors and Affiliations
Contributions
LXF designed the plan, investigated the research, collected and processed the plant material, and conducted the experiments; LQX undertook the writing, first draft preparation. CY and LTF undertook data analysis; ZYN did the review and editing. All the authors approved the final version of the manuscript.
Corresponding author
Ethics declarations
Ethical Approval
Not applicable.
Consent to Participate
The authors have agreed to participate in the publication of the paper.
Consent for Publication
All authors have agreed to publish the paper.
Conflict of Interest
The authors declare no competing interests.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Li, X., Li, Q., Cui, Y. et al. Establishment of tissue culture regeneration system of Ficus tikoua. In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-024-10414-3
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11627-024-10414-3