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Application of Immobilized β-Glucosidase from Candida boidinii in the Hydrolysis of Delignified Sugarcane Bagasse

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Abstract

Candida boidinii is a methylotrophic yeast with wide geographical distribution. In the present study, the microorganism was isolated from the Bahian semiarid and the enzymatic extract containing β-glucosidase was obtained through submerged fermentation. Response surface methodology was employed to optimize of fermentation medium. The higher production of β-glucosidase was obtained after 71 h of fermentation in an optimized medium composed of 3.35% glucose, 1.78% yeast extract and 1% peptone. The optimum pH and temperature of enzymatic activity were 6.8 (citrate–phosphate buffer) and 71.7 °C, respectively. Salts tested (10 mM) CaCl2, Na2SO4 and ZnSO4 promotes the increase of 91%, 45% and 80% of activity, respectively. The enzyme retained 64% ± 2.3 of its initial activity after 1 h heating at 90 °C. The production of reducing sugars was 95.94% after 24 h of hydrolysis and, with the addition of metal ions, this value increased more than 2 times. Among the supports analyzed for immobilization, chitosan showed higher residual activity during reuse. The immobilized enzyme showed higher activity at 60 °C with pH 6 and preserved almost 100% of the initial activity after 30 min at 70 °C.

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Acknowledgements

We thank the Biotechnology Graduate Program of State University of Feira de Santana (UEFS/FIOCRUZ), the Coordination for the Improvement of Higher Education Personnel (CAPES) for a doctoral scholarship (88882.447813/2019-01), the Bahia State Research Support Foundation (FAPESB), and the Brazilian National Council for Scientific and Technological (CNPq).

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  1. Enzymology and Fermentation Technology Laboratory, Health Department, State University of Feira de Santana, Transnordestina Ave., km 0, BR 116, Feira de Santana, Bahia, 44036-900, Brazil

    Larissa Emanuelle da Silva Almeida & Sandra Aparecida de Assis

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  1. Larissa Emanuelle da Silva Almeida
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  2. Sandra Aparecida de Assis
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Larissa E. S. Almeida and Sandra A. Assis. The first draft of the manuscript was written by Larissa E. S. Almeida and and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sandra Aparecida de Assis.

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da Silva Almeida, L.E., de Assis, S.A. Application of Immobilized β-Glucosidase from Candida boidinii in the Hydrolysis of Delignified Sugarcane Bagasse. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-024-01223-8

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