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Direct Determination of the Structure of Single Biopolymer Molecules Using Nanopore Sequencing

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Abstract

This review highlights operational principles, features, and modern aspects of the development of third-generation sequencing technology of biopolymers focusing on the nucleic acids analysis, namely the nanopore sequencing system. Basics of the method and technical solutions used for its realization are considered, from the first works showing the possibility of creation of these systems to the easy-to-handle procedure developed by Oxford Nanopore Technologies company. Moreover, this review focuses on applications, which were developed and realized using equipment developed by the Oxford Nanopore Technologies, including assembly of whole genomes, methagenomics, direct analysis of the presence of modified bases.

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Funding

This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation, grant no. 075-15-2021-1396, October 26, 2021.

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Authors and Affiliations

  1. Faculty of Chemistry, Lomonosov Moscow State University, 119991, Moscow, Russia

    Anna K. Berkovich, Olga A. Pyshkina, Anna A. Zorina, Vladimir A. Rodin, Tatyana V. Panova, Vladimir G. Sergeev & Maria E. Zvereva

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  1. Anna K. Berkovich
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  2. Olga A. Pyshkina
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  3. Anna A. Zorina
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  4. Vladimir A. Rodin
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  5. Tatyana V. Panova
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  6. Vladimir G. Sergeev
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  7. Maria E. Zvereva
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Contributions

M.E.Z. and V.K.S. concept and supervision of the study; O.A.P. analysis of the literature on the structure and organization of nanopores; A.A.Z. and V.A.R analysis of the literature on the history of method development and preparation of illustrations; T.V.P. information on the technology applications; A.K.B preparation of the text and editing the paper.

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Correspondence to Anna K. Berkovich.

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This work does not contain any studies involving human and animal subjects. The authors of this work declare that they have no conflicts of interest.

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Translated from Uspekhi Biologicheskoi Khimii, 2024, Vol. 64, pp. 449-478.

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Berkovich, A.K., Pyshkina, O.A., Zorina, A.A. et al. Direct Determination of the Structure of Single Biopolymer Molecules Using Nanopore Sequencing. Biochemistry Moscow 89 (Suppl 1), S234–S248 (2024). https://doi.org/10.1134/S000629792414013X

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