Abstract
Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in Arabidopsis was investigated. We showed that petal senescence in aos mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in dad1 and coi1 mutants. However, MYB21/24 and MYC2/3/4, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that AOS altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, bHLH13, bHLH17 and URH2 were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in Arabidopsis. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.
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Accession numbers
Sequence data from this article can be found in GenBank under the following accession numbers: AOS (At5g42650), DAD1 (At2g44810), COI1 (At2g39940), MYB21 (At3g27810), MYB24 (At5g40350), MYB108 (At3g06490), AMS (At2g16910), MYC2 (At1g32640), MYC3 (At5g46760), MYC4 (At4g17880), ARF17 (At1g77850), bHLH13 (At1g01260), bHLH17 (At2g46510), URH2 (At1g05620), SAG13 (At2g29350), SAG21 (At4g02380), SAG24 (At1g66580), SAG29 (At5g13170), SRG1 (At1g17020).
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Acknowledgements
We thank Daoxin Xie for giving myc2, myc3 mutant seeds, Benke kuai and Dingyu Zhang for giving myc4, myc2/3, myc2/4, myc3/4 and myc2/3/4 mutant seeds. We thank Xin Zhou and Jufang Gao for helpful suggestions and support.
Funding
This work was supported by Shanghai Engineering Research Center of Plant Germplasm Resources (Grant number 17DZ2252700), Science and Technology Research Project of Jiangxi Education Department (Grant number GJJ212801) and Science and Technology Plan Project of Jingdezhen (Grant number 20224SF007-1).
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Wu, L., Wang, K., Chen, M. et al. ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis. Physiol Mol Biol Plants 30, 199–212 (2024). https://doi.org/10.1007/s12298-024-01425-w
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DOI: https://doi.org/10.1007/s12298-024-01425-w