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Successful Treatment of Recurrent Clostridioides difficile Infection Using a Novel, Drinkable, Oral Formulation of Fecal Microbiota

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Abstract

Background

Fecal microbiota transplants can be administered orally in encapsulated form or require invasive procedures to administer liquid formulations. There is a need for an oral liquid formulation of fecal microbiota for patients who are unable to swallow capsules, especially if they require multiple, repeated administrations.

Aims

These studies were conducted to develop a protocol to manufacture an organoleptically acceptable powdered fecal microbiota formulation that can be suspended in a liquid carrier and used for fecal microbiota transplantation.

Methods

Several processing steps were investigated, including extra washes of microbiota prior to lyophilization and an addition of a flavoring agent. The viability of bacteria in the transplant formulation was tested using live/dead microscopy staining and engraftment into antibiotic-treated mice. After development of a clinical protocol for suspension of the powdered microbiota, the new formulation was tested in three elderly patients with recurrent Clostridioides difficile infections and who have difficulties in swallowing capsules. Changes in the microbial community structure in one of the patients were characterized using 16S rRNA gene profiling and engraftment analysis.

Results

The processing steps used to produce an organoleptically acceptable suspension of powdered fecal microbiota did not result in loss of its viability. The powder could be easily suspended in a liquid carrier. The use of the new formulation was associated with abrogation of the cycle of C. difficile infection recurrences in the three patients.

Conclusion

We developed a novel organoleptically acceptable liquid formulation of fecal microbiota that is suitable for use in clinical trials for patients with difficulties in swallowing capsules.

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Acknowledgments

Research funding for this work was supported by Finch Therapeutics and philanthropic funding from the Frank J. and Eleanor A. Maslowski Trust and non-profit Achieving Cures Together.

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Author notes

  1. Michael J. Sadowsky and Alexander Khoruts have contributed equally.

Authors and Affiliations

  1. BioTechnology Institute, University of Minnesota, St. Paul, MN, USA

    Michael J. Sadowsky, Prince P. Mathai, Christopher Staley & Alexander Khoruts

  2. Department of Soil, Water, and Climate, Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN, USA

    Michael J. Sadowsky

  3. Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Minnesota, 3-184 Wallin Medical BioSciences Building, 2101 6th St. S.E., Minneapolis, MN, 55416, USA

    Michael J. Sadowsky, Michael Matson, Maradi Pho, Clayton Evert, Melissa Weldy & Alexander Khoruts

  4. Center for Immunology, University of Minnesota, Minneapolis, MN, USA

    Alexander Khoruts

  5. Department of Surgery, Division of Basic and Translational Research, University of Minnesota, Minneapolis, MN, USA

    Christopher Staley

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  1. Michael J. Sadowsky
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  2. Michael Matson
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Correspondence to Alexander Khoruts.

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Sadowsky, M.J., Matson, M., Mathai, P.P. et al. Successful Treatment of Recurrent Clostridioides difficile Infection Using a Novel, Drinkable, Oral Formulation of Fecal Microbiota. Dig Dis Sci (2024). https://doi.org/10.1007/s10620-024-08351-7

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  • DOI: https://doi.org/10.1007/s10620-024-08351-7

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