Abstract
Quinoa, a dicotyledonous annual herb belonging to the subfamily Amaranthaceae, has remarkable resistance to cold, drought, and barren conditions. However, few studies have reported on quinoa root metabolism under waterlogging stress. In this study, quinoa strains were treated with waterlogging, and the control group was exposed to normal water management conditions; waterlog-resistant (Dianli-188) and waterlog-sensitive (Dianli-60) strains were selected. Ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to analyze the root systems of four groups of quinoa seedlings (Dianli-60 treatment group: TR4; Dianli-60 control group: CR4; Dianli-188 treatment group: TR5; Dianli-188 control group: CR5). In total, 1044 metabolites were detected. Kyoto Encyclopedia of Genes and Genomes analysis was used to identify 648 differential metabolites involved in biosynthesis. In addition, we found that alanine, aspartate, and glutamate metabolism and glycolysis, tricarboxylic cycle, and butanoate metabolism pathways were related to the synthesis of γ-aminobutyric acid (GABA). The metabolites of C5-branched dibasic acid metabolism, urea cycle, starch and sucrose metabolism, phenolic metabolism, and flavonoid biosynthesis pathways were found to participate in the waterlogging stress response. Sucrose, glucose, and trehalose provide sufficient energy for adventitious root production. GABA regulates phenylpropane metabolism, including regulation of phenolic acids and flavonoids to enhance the antioxidant capacity of quinoa in response to waterlogging stress. This study provided a theoretical basis for breeding quinoa waterlog-resistant varieties, we can screen waterlogging-resistant strains based on the content of these metabolites in future. Thereby accelerating the breeding process of quinoa.
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Data Availability
The datasets generated for this study are available upon request from the corresponding authors.
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Acknowledgements
We would like to thank Editage (www.editage.cn) for English language editing and Wuhan MetWare Biotechnology Co., Ltd. (www.metware.cn) for the metabolomic analyses.
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This work was supported by the Yunnan Expert Workstation (202205AF150001).
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Yirui Guo: Writing—original draft, methodology. Qianchao Wang: Writing the original draft and formal analysis. Heng Xie: Conceptualization, writing—review, and editing. Xuesong Zhang: Methodology and visualization. Tingzhi Huang: Field sample collection and plant material preparation. Ping Zhang: Formal analysis and investigation. Junna Liu: Formal analysis and investigation. Li Li: Investigation. Peng Qin: Supervision, project administration, and funding acquisition.
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Guo, Y., Wang, Q., Xie, H. et al. Analysis of Root Response Mechanism of Quinoa Seedlings to Waterlogging Stress Based on Metabolome. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11260-8
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DOI: https://doi.org/10.1007/s00344-024-11260-8