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Growth of Mesenchymal Stem Cells on Oriented Microstructured Films and Electrospun Scaffolds

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Abstract

The study involved the fabrication of films with different roughness and scaffolds made of poly-3-hydroxybutyrate using various methods. Chaotic and oriented scaffolds with varying fiber thickness were obtained through the electrospinning method, depending on the polymer concentration and electrospinning parameters. Films with different surface roughness were obtained using spin coating and self-assembly methods. It was demonstrated that the varying microstructure of the surface does not affect the growth of mesenchymal stem cells over the course of 1 week; however, it does influence the morphology of the adhered cells.

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ACKNOWLEDGMENTS

Equipment used in the present work was courtesy of the Collective Use Center at Moscow State University (including scanning electron microscopes); Shared-Access Equipment Center at the Research Center of Biotechnology of the Russian Academy of Sciences, and the Institute of Chemistry of New Materials of the National Academy of Sciences of Belarus. Electron microscopy was performed using 3D-EMS specialized equipment in Moscow State University.

Funding

This research was supported by the Russian Science Foundation, project number. 20-64-47008 (pertaining to the development of electrospun scaffolds and study of their morphology and biocompatibility in vitro; Faculty of Biology, Moscow State University), and by the Ministry of Science and Higher Education, grant agreement number 075-15-2021-588 dated June 1, 2021 (pertaining to research and analysis using Raman spectroscopy; Tomsk Polytechnic University).

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Authors and Affiliations

  1. Faculty of Biology, Shenzhen MSU-BIT University, Dayun New Town, Longgang District, 518172, Shenzhen, Guangdong Province, People’s Republic of China

    I. V. Demianova & E. A. Akoulina

  2. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    E. A. Akoulina, I. I. Zharkova, V. V. Voinova, D. V. Chesnokova, A. M. Hossain, K. V. Shaitan & A. P. Bonartsev

  3. Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    T. K. Makhina, G. A. Bonartseva & A. P. Bonartsev

  4. Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 220084, Minsk, Belarus

    V. I. Kulikouskaya & V. V. Nikalaichuk

  5. Physical Materials Science and Composite Materials Research Center, Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050, Tomsk, Russia

    Yu. R. Mukhortova, A. S. Pryadko, M. A. Surmeneva & R. A. Surmenev

  6. Piezo- and Magnetoelectric Materials Research Center, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050, Tomsk, Russia

    M. A. Surmeneva & R. A. Surmenev

Authors
  1. I. V. Demianova
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  2. E. A. Akoulina
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  5. D. V. Chesnokova
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  6. A. M. Hossain
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  7. T. K. Makhina
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  8. G. A. Bonartseva
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  9. V. I. Kulikouskaya
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  10. V. V. Nikalaichuk
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  11. Yu. R. Mukhortova
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  12. A. S. Pryadko
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  13. M. A. Surmeneva
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  14. R. A. Surmenev
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  15. K. V. Shaitan
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  16. A. P. Bonartsev
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Corresponding author

Correspondence to E. A. Akoulina.

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The authors of this work declare that they have no conflicts of interest.

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Translated by E. Kuznetsova

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Demianova, I.V., Akoulina, E.A., Zharkova, I.I. et al. Growth of Mesenchymal Stem Cells on Oriented Microstructured Films and Electrospun Scaffolds. Moscow Univ. Biol.Sci. Bull. 78 (Suppl 1), S34–S39 (2023). https://doi.org/10.3103/S0096392523700189

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  • DOI: https://doi.org/10.3103/S0096392523700189

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