Abstract
The paper reports an example of a successful upgrade of the JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope, which can be used to optimize sample preparation and to assess preparation quality. As a result of the instrument upgrade, it is possible to obtain subnanometer resolution of protein molecule reconstructions (within 8 Å). The role of graphene and amorphous carbon substrates in preventing the effect of preferential orientation of protein particles in a frozen sample is discussed.
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Funding
Microscope upgrading was supported by the Ministry of Science and Higher Education of the Russian Federation. Analysis of samples and obtaining three-dimensional structures of tick-borne encephalitis virus was supported by the Moscow State University Development Program (project no. 23A-Sh04-01). Development of nucleosome measurement techniques was supported by the Russian Science Foundation (project no. 19-74-30003).
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Moiseenko, A.V., Egorov, A.M., Shaitan, K.V. et al. Cryo-Electron Microscopy at the Biological Faculty of Moscow State University. Moscow Univ. Biol.Sci. Bull. 78 (Suppl 1), S59–S63 (2023). https://doi.org/10.3103/S0096392523700232
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DOI: https://doi.org/10.3103/S0096392523700232