Abstract
Photosystem II (PSII) is one of the main pigment-protein complexes of photosynthesis which is highly sensitive to unfavorable environmental factors. The heterogeneity of PSII properties is essential for the resistance of autotrophic organisms to stress factors. Assessment of the PSII heterogeneity may be used in environmental monitoring for on-line detection of contamination of the environment. We propose an approach to assess PSII oxygen-evolving complex and light-harvesting antenna heterogeneity that is based on mathematical modeling of the shape of chlorophyll a fluorescence rise of 3-(3,4-dichlorophenyl)-1,1-dimethylurea-treated samples. The hierarchy of characteristic times of the processes considered in the model makes it possible to reduce the model to a system of three ordinary differential equations. The analytic solution of the reduced three-state model is expressed as a sum of two exponential functions, and it exactly reproduces the solution of the complete system within the time range from microseconds to hundreds of milliseconds. The combination of several such models for reaction centers with different properties made it possible to use it as an instrument to study PSII heterogeneity. PSII heterogeneity was studied for Chlamydomonas at different intensities of actinic light, for Scenedesmus under short-term heating, and for Chlorella grown in nitrate-enriched and nitrate-depleted media.
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Data availability
Data sets generated during the current study are available from the corresponding author on reasonable request.'
Abbreviations
- DCMU:
-
3-(3,4-Dichlorophenyl)-1,1-dimethylurea,
- I:
-
Pheophytin (primary electron acceptor in PSII),
- LHCII:
-
PSII light harvesting complex,
- OEC:
-
Oxygen evolving complex,
- P680 :
-
PSII RC pigment,
- PSII:
-
Photosystem II,
- QA :
-
Primary quinone electron acceptor,
- QB :
-
Secondary quinone electron acceptor,
- RC:
-
Reaction center,
- Y:
-
Tyrosine Z
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This research was carried out with support by the Russian Science Foundation (project 22-11-00009).
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Conceptualization: Plyusnina T.Yu., Rubin A.B., Riznichenko G.Yu., Khruschev S.S; Mathematical model creation and analysis: Plyusnina T.Yu., Khruschev S.S; Microalgae cultivation: Voronova E.N., Volgusheva A.A.; Sample preparation and fluorescence induction measurements: Degtereva N.S.; Model fitting and simulation: Plyusnina T.Yu., Degtereva N.S.; Writing—original draft preparation: Plyusnina T.Yu., Khruschev S.S; Writing—review and editing: all authors.
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Plyusnina, T.Y., Khruschev, S.S., Degtereva, N.S. et al. Three-state mathematical model for the assessment of DCMU-treated photosystem II heterogeneity. Photosynth Res 159, 303–320 (2024). https://doi.org/10.1007/s11120-024-01077-7
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DOI: https://doi.org/10.1007/s11120-024-01077-7