Abstract
Background
Fungi play a crucial role in the development of rapeseed and influencing the functioning of agroecosystems. However, the fungal composition and function in spring rapeseed on the Qinghai-Tibet Plateau (QTP) remain unclear.
Methods
The agronomic traits, rhizosphere soil properties, and fungal communities across multiple niches within seven cultivars of Brassica rapa L. and Brassica napus L. were investigated using cultivation, physiological assay, and high-throughput sequencing.
Results
We found significant differences in growth indices, yields and fungal diversity among leaf, stem, and root compartments of B. napus, especially in late-maturing cultivars, compared to B. rapa. Interestingly, defense-related indices and fungal diversity in rhizosphere soil exhibited the opposite trend. A total of 12 phyla, 55 orders and 200 genera were identified. The predominant genera were Olpidium (66.29%), Lactarius (18.37%), Verticillium (1.99%), Mortierella (0.78%) and Cystofilobasidium (0.56%). The rhizosphere soil had a higher abundance of Olpidium and Mortierella than other ecological niches, while the stem harbored more Lactarius and Verticillium. In addition, 46 key genera were widespread across all niches and cultivars, and 19 biomarkers were significantly enriched, with saprotroph-symbiotroph, symbiotrophs and pathotroph-symbiotroph trophic groups dominating. Alternaria isolated from rapeseed, which was also identified as one of the most abundant, key and biomarker genera, effectively facilitated the germination of B. rapa under chilling and drought stress.
Conclusions
The joint influence of numerous important fungal genera can promote the growth and yield of rapeseed. Our results provide valuable insights into the complex relationship between rapeseed and fungal communities on the QTP.
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Data availability
The raw sequence data in this paper have been deposited in the Sequence Read Archive of NCBI under the accession number PRJNA983949.
References
Chen JW, Wu Y, Zhuang X, Guo JJ, Hu X, Xiao JL (2022) Diversity analysis of leaf endophytic fungi and rhizosphere soil fungi of Korean Epimedium at different growth stages. Environ Microbiome 17:52. https://doi.org/10.1186/s40793-022-00446-w
China Statistical Yearbook (2022) National Bureau of Statistics of China. Available via DIALOG. http://www.stats.gov.cn/sj/ndsj/2022/indexch.htm. Accessed 15 Aug 2023
Chojnacka A, Jonczak J, Oktaba L, Pawłowicz E, Regulska E, Słowińska S, Olejniczak I, Oktaba J, Kruczkowska B, Jankiewicz U (2023) Dynamics of fungal community structure in a silver birch (Betula pendula Roth) succession chronosequence on poor-quality post-arable soil. Agric Ecosyst Environ 342:108225. https://doi.org/10.1016/j.agee.2022.108225
Costa D, Fernandes T, Martins F, Pereira JA, Tavares RM, Santos PM, Baptista P, Lino-Neto T (2021) Illuminating Olea europaea L. endophyte fungal community. Microbiol Res 245:126693. https://doi.org/10.1016/j.micres.2020.126693
Das GG, Malek MA, Shamsuddin AKM, Sagor GHM (2022) Development of high yielding early matured and shattering tolerant Brassica napus L. through interspecific hybridization between B. rapa L. and B. oleracea L. Genet Resour Crop Evol 69:1009–1018. https://doi.org/10.1007/s10722-021-01277-3
Dong M, Shi L, Xie Z, Lian L, Zhang J, Jiang Z, Wu C (2023) Shifts in the diversity of root endophytic microorganisms across the life cycle of the ratooning rice Jiafuzhan. Front Microbiol 14:1161263. https://doi.org/10.3389/fmicb.2023.1161263
Duan DD, Jiang FF, Lin WH, Tian Z, Wu NN, Feng XX, Chen T, Nan ZB (2022) Effects of drought on the growth of Lespedeza davurica through the alteration of soil microbial communities and nutrient availability. J Fungi 8(4):384. https://doi.org/10.3390/jof8040384
Fan K, Weisenhorn P, Gilbert JA, Shi Y, Bai Y, Chu H (2018) Soil pH correlates with the co-occurrence and assemblage process of diazotrophic communities in rhizosphere and bulk soils of wheat fields. Soil Biol Biochem 121:185–192. https://doi.org/10.1016/j.soilbio.2018.03.017
Gao CC, Zhang Y, Li HM, Gao Q, Cheng YD, Ogunyemi SO, Guan JF (2022a) Fruit bagging reduces the postharvest decay and alters the diversity of fruit surface fungal community in ‘Yali’ pear. BMC Microbiol 22:239. https://doi.org/10.1186/s12866-022-02653-4
Gao YY, Peng SJ, Hang Y, Xie GF, Ji N, Zhang MS (2022b) Mycorrhizal fungus Coprinellus disseminatus influences seed germination of the terrestrial orchid Cremastra appendiculata (D. Don) Makino. Sci Hortic 293:110724. https://doi.org/10.1016/j.scienta.2021.110724
Griffiths RI, Whiteley AS, O’donnell AG, Bailey M (2000) Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and RNA-based microbial community composition. Appl Environ Microbiol 66(12):5488–5491. https://doi.org/10.1128/AEM.66.12.5488-5491.2000
Guo Y, Jud W, Weikl F, Ghirardo A, Junker RR, Polle A, Benz P, Pritsch K, Schnitzler J-P, Rosenkranz M (2021) Volatile organic compound patterns predict fungal trophic mode and lifestyle. Commun Biol 4:673. https://doi.org/10.1038/s42003-021-02198-8
He MJ, Sun WM, Cui SL, Mu GJ, Liu LF, Guo W (2021) Analysis of microbial diversity and community structure of peanut pod and its surrounding soil in peanut rot epidemic area. Curr Microbiol 78(3):2173–2182. https://doi.org/10.1007/s00284-021-02471-3
Hilton S, Picot E, Schreiter S, Bass D, Norma K, Oliver AE, Moore JD, Mauchline TH, Mills PR, Teakle GR, Clark IM, Hirsxh PR, van der Gast CJ, Bending GD (2021) Identification of microbial signatures linked to oilseed rape yield decline at the landscape scale. Microbiome 9:19. https://doi.org/10.1186/s40168-020-00972-0
Jia Y, Liu Y, Hu W, Cai W, Zheng Z, Luo C, Li D (2023) Development of Candida autochthonous starter for cigar fermentation via dissecting the microbiome. Front Microbiol 14:1138877. https://doi.org/10.3389/fmicb.2023.1138877
Jiménez JA, Novinscak A, Filion M (2020) Pseudomonas fluorescens LBUM677 differentially increases plant biomass, total oil content and lipid composition in three oilseed crops. J Appl Microbiol 128(4):1119–1127. https://doi.org/10.1111/jam.14536
Kioukis A, Michalopoulou VA, Briers L, Pirintsos S, Studholme DJ, Pavlidi P, Sarris PF (2020) Intraspecific diversification of the crop wild relative Brassica cretica Lam. using demographic model selection. BMC Genomics 21:48. https://doi.org/10.1186/s12864-019-6439-x
Lay CY, Hamel C, St-Arnaud M (2018) Taxonomy and pathogenicity of Olpidium brassicae and its allied species. Fungal Biol 122(9):837–846. https://doi.org/10.1016/j.funbio.2018.04.012
Lebreton A, Tang NW, Kuo A, LaButti K, Andreopoulos W, Drula E, Miyauchi S, Barry K, Clum A, Lipzen A, Mousain D, Ng V, Wang R, Dai YC, Henrissat B, Grigoriev IV, Guerin-Laguette A, Yu FQ, Martin FM (2022) Comparative genomics reveals a dynamic genome evolution in the ectomycorrhizal milk-cap (Lactarius) mushrooms. New Phytol 235:306–319. https://doi.org/10.1111/nph.18143
Li J, Duan YF, Sun NL, Wang L, Feng SS, Fang YJ, Wang YP (2021) The miR169n-NF-YA8 regulation module involved in drought resistance in Brassica napus L. Plant Sci 313:111062. https://doi.org/10.1016/j.plantsci.2021.111062
Li Q, He GX, Wen T, Zhang DG, Liu XN (2022a) Distribution pattern of soil fungi community diversity in alpine meadow in Qilian Mountains of eastern Qinghai-Tibetan Plateau. Ecol Indic 141(1):109054. https://doi.org/10.1016/j.ecolind.2022.109054
Li WY, Lei XJ, Zhang R, Cao QJ, Yang H, Zhang NQ, Liu SL, Wang YP (2022b) Shifts in rhizosphere microbial communities in Oplopanax elatus Nakai are related to soil chemical properties under different growth conditions. Sci Rep 12(1):11485. https://doi.org/10.1038/s41598-022-15340-1
Li Y, He X, Yuan H, Lv G (2022c) Differed growth stage dynamics of root-associated bacterial and fungal community structure associated with halophytic plant Lycium ruthenicum. Microorganisms 10(8):1644. https://doi.org/10.3390/microorganisms10081644
Li ZZ, Yang YZ, Zheng H, Hu BA, Dai XH, Meng N, Zhu JY, Yan DN (2023a) Environmental changes drive soil microbial community assembly across arid alpine grasslands on the Qinghai-Tibetan Plateau, China. Catena 228:107175. https://doi.org/10.1016/j.catena.2023.107175
Li YL, Bazghaleh N, Vail A, Mamet S, Siciliano S, Helgason B (2023b) Root and rhizosphere fungi associated with the yield of diverse Brassica napus genotypes. Rhizosphere 25:100677. https://doi.org/10.1016/j.rhisph.2023.100677
Lin Q, Wang Y, Li MM, Xu ZX, Li L (2022) Ecological niche selection shapes the assembly and diversity of microbial communities in Casuarina equisetifolia L. Front Plant Sci 13:988485. https://doi.org/10.3389/fpls.2022.988485
Liu Y, Xu H, Wen XX, Liao YC (2016) Effect of polyamine on seed germination of wheat under drought stress is related to changes in hormones and carbohydrates. J Integr Agric 15(12):2759–2774. https://doi.org/10.1016/S2095-3119(16)61366-7
Liu SE, Wang H, Tian P, Yao X, Sun H, Wang QK, Delgado-Baquerizo M (2020) Decoupled diversity patterns in bacteria and fungi across continental forest ecosystems. Soil Biol Biochem 144:107763. https://doi.org/10.1016/j.soilbio.2020.107763
Liu XY, Li WX, Wang MH, Cao YS, Zhang T, Hu HG, Cheng XY, Yan Q (2023) Establishment of hairy root system of transgenic IRT1 brassica campestris L. and preliminary study of its effect on cadmium enrichment. Int J Phytoremediat 25(11):1455–1462. https://doi.org/10.1080/15226514.2022.2164247
Lu Q, Hu C, Cai L, Wu C, Zhang H, Wei L, Zhang T, Hu H, Liu S, Lei J, Ge T, Dai L, Yang J, Chen J (2022) Changes in soil fungal communities after onset of wheat yellow mosaic virus disease. Front Bioeng Biotechnol 10:1033991. https://doi.org/10.3389/fbioe.2022.1033991
Maitra P, Zheng Y, Wang YL, Mandal D, Lü PP, Gao C, Joshua B, Ji NN, Li XC, Guo LD (2021) Phosphorus fertilization rather than nitrogen fertilization, growing season and plant successional stage structures arbuscular mycorrhizal fungal community in a subtropical forest. Biol Fert Soils 57:685–697. https://doi.org/10.1007/s00374-021-01554-4
Meng JL, Shi SW, Gan L, Li ZY, Qu XS (1998) The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. Napus. Euphytica 103:329–333. https://doi.org/10.1023/A:1018646223643
Mussagy CU, Ribeiro HF, Santos-Ebinuma VC, Schuur B, Pereira JFB (2022) Rhodotorula sp.–based biorefinery: a source of valuable biomolecules. Appl Microbiol Biotechnol 106:7431–7447. https://doi.org/10.1007/s00253-022-12221-5
Oilseeds: World Markets and Trade (2023) United States Department of Agriculture. Available via DIALOG. https://apps.fas.usda.gov/psdonline/app/index.html#/app/downloads. Accessed 10 Aug 2023
Peng QQ, Li CJ, Song ML, Nan ZB (2013) Effects of seed hydropriming on growth of Festuca sinensis infected with Neotyphodium endophyte. Fungal Ecol 6(1):83–91. https://doi.org/10.1016/j.funeco.2012.08.00
Qadir M, Hussain A, Shah M, Hamayun M, Lqbal A, Nadia (2023) Enhancement of chromate phytoremediation and soil reclamation potential of Brassica campestris L. by aspergillus Niger. Environ Sci Pollut R 30:9471–9482. https://doi.org/10.1007/s11356-022-22678-6
Qu ZL, Santalahti M, Köster K, Berninger F, Pumpanen J, Heinonsalo J, Sun H (2021) Soil fungal community structure in boreal pine forests: from southern to subarctic areas of Finland. Front Microbiol 12:653896. https://doi.org/10.3389/fmicb.2021.653896
Qu ZL, Braima A, Liu B, Ma Y, Sun H (2022) Soil fungal community structure and function shift during a disease-driven forest succession. Microbiol Spectr 10(5):e00795–e00722. https://doi.org/10.1128/spectrum.00795-22
Rivas GA, Semorile L, Delfederico L (2022) Microbial diversity of the soil, rhizosphere and wine from an emerging wine-producing region of Argentina. LWT 153:112429. https://doi.org/10.1016/j.lwt.2021.112429
Shi YW, Yang HM, Chu M, Niu XX, Wang N, Lin Q, Lou K, Zuo CG, Wang JY, Zou Q, Zhang YM (2021) Differentiation and variability in the rhizosphere and endosphere microbiomes of healthy and diseased cotton (Gossypium sp.). Front Microbiol 12:765269. https://doi.org/10.3389/fmicb.2021.765269
Sun X, Li JL, He C, Li XC, Guo LD (2021) Specific network and phylosymbiosis pattern in endophyte community of coastal halophytes. Fungal Ecol 53:101088. https://doi.org/10.1016/j.funeco.2021.101088
Tian Y, Zhou Y, Huang S, Li J, Zhao K, Li XH, Wen XC, Li XA (2019) Fecal microbiota transplantation for ulcerative colitis: a prospective clinical study. BMC Gastroenterol 19:116. https://doi.org/10.1186/s12876-019-1010-4
Tkacz A, Cheema J, Chandra G, Grant A, Poole PS (2015) Stability and succession of the rhizosphere microbiota depends upon plant type and soil composition. ISME J 9:2349–2359
Wang BX, Sugiyama S (2020) Phylogenetic signal of host plants in the bacterial and fungal root microbiomes of cultivated angiosperms. Plant J 104(2):522–531. https://doi.org/10.1111/tpj.14943
Wang L, Mühling K-H, Schulte auf’m Erley G (2016) Nitrogen efficiency and leaf nitrogen remobilisation of oilseed rape lines and hybrids. Ann Appl Biol 169(1):125–133. https://doi.org/10.1111/aab.12286
Wang Y, Li T, Li CW, Song FQ (2020) Differences in microbial community and metabolites in litter layer of plantation and original Korean pine forests in north temperate zone. Microorganisms 8:2023. https://doi.org/10.3390/microorganisms8122023
Wang XY, Li W, Xiao YT, Cheng AQ, Shen TM, Zhu M, Yu LJ (2021) Abundance and diversity of carbon-fixing bacterial communities in karst wetland soil ecosystems. Catena 204:105418. https://doi.org/10.1016/j.catena.2021.105418
Wang Y, Wang LW, Suo M, Qiu ZJ, Wu H, Zhao M, Yang HY (2022) Regulating root fungal community using Mortierella alpina for fusarium oxysporum resistance in Panax ginseng. Front Microbiol 13:850917. https://doi.org/10.3389/fmicb.2022.850917
Whipps JM, Hand P, Pink D, Bending GD (2008) Phyllosphere microbiology with special reference to diversity and plant genotype. J Appl Microbiol 105(6):1744–1755. https://doi.org/10.1111/j.1365-2672.2008.03906.x
Wiriya J, Rangjaroen C, Teaumroong N, Sungthong R, Lumyong S (2020) Rhizobacteria and arbuscular mycorrhizal fungi of oil crops (physic nut and Sacha Inchi): a cultivable-based assessment for abundance, diversity, and plant growth-promoting potentials. Plants 9(12):1773. https://doi.org/10.3390/plants9121773
Wu K, Xu WX, Yang WK (2020) Effects of precipitation changes on soil bacterial community composition and diversity in the Junggar desert of Xinjiang, China. Peer J 8:e8433. https://doi.org/10.7717/peerj.8433
Yao Y, Zhao Y, Yao X, Bai Y, An L, Li X, Wu K (2022) Impacts of continuous cropping on fungal communities in the rhizosphere soil of Tibetan barley. Front Microbiol 13:755720. https://doi.org/10.3389/fmicb.2022.755720
Ye GP, Lin YX, Luo JF, Di HJ, Lindsey S, Liu DY, Fan JB, Ding WX (2020) Responses of soil fungal diversity and community composition to long-term fertilization: field experiment in an acidic Ultisol and literature synthesis. Appl Soil Ecol 145:103305. https://doi.org/10.1016/j.apsoil.2019.06.008
Zan LX, Li KX, Jia YP, Du DZ (2023) Screening and evaluation of three restorer lines used to create synthetic hybrids of extra-early-maturing Brassica napus. Euphytica 219:45. https://doi.org/10.1007/s10681-023-03175-4
Zhang M, Chai L, Huang M, Jia WQ, Huang Y (2020) Deciphering the archaeal communities in tree rhizosphere of the Qinghai-Tibetan Plateau. BMC Microbiol 20:235. https://doi.org/10.1186/s12866-020-01913-5
Zheng H, Zhang P, Qin J, Guo J, Deng J (2023) High-throughput sequencing-based analysis of the composition and diversity of endophytic bacteria community in tubers of Gastrodia elata f.glauca. Front Microbiol 13:1092552. https://doi.org/10.3389/fmicb.2022.1092552
Zhong F, Fan X, Ji W, Hai Z, Hu N, Li X, Liu G, Yu C, Chen Y, Lian B, Wei H, Zhang J (2022) Soil fungal community composition and diversity of culturable endophytic fungi from plant roots in the reclaimed area of the eastern coast of China. J Fungi 8:124. https://doi.org/10.3390/jof8020124
Acknowledgments
This study was supported by Special Project for the Transformation of Scientific and Technological Achievements of Qinghai Province (2024-SF-130).
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Peng Q and Xie Z conceived the study. Tang G performed field work. Jiang H provided good suggestions for the study. Guo J, Mao Y, Wang B, Meng Q and Yang J provided continuous help for laboratory work. Jia S and La M collected raw data. Peng Q wrote the manuscript. All authors contributed to the discussion and final version of the manuscript.
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Peng, Q., Xie, Z., Tang, G. et al. Fungal community composition and function in different spring rapeseeds on the Qinghai-Tibet Plateau, China. Plant Soil (2024). https://doi.org/10.1007/s11104-024-06610-0
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DOI: https://doi.org/10.1007/s11104-024-06610-0