Abstract
Sea buckthorn (Hippophae rhamnoides), a hardy deciduous shrub of the family Elaeagnaceae, is known for its economic and ecological importance due to its wide use in medicine, food, windbreaks, and sand fixation. In this study, the first genetic linkage map of H. rhamnoides was constructed based on an F1 population (H. rhamnoides subsp. mongolica ‘wulangemu’ × H. rhamnoides subsp. sinensis ‘wucixiong’) by using start codon targeted (SCoT), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) markers. The linkage map contained 1143 markers with a total length of 1889.46 centimorgans (cM), including 12 linkage groups (LG) and covering 97.17% of the estimated genome, with an average marker density of 1.67 cM. According to the phenotypic variation analysis, 61 QTLs were identified. Among these QTLs, there were 18 QTLs for seedling height, 27 QTLs for ground diameter, two QTLs for primary branching angle, 11 QTLs for leaf width, and 3 QTLs for leaf shape index. The range of phenotypic variation explained by these QTLs was 10.7 ~ 21.9%. The QTLs identified by high-density linkage mapping reported in this study will be useful for the molecular breeding of sea buckthorn.
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Acknowledgements
This work was financially supported by Applied Basic Research in Shanxi Province of China (No. 201801D221292 and No. 201901D211360), Basic Research Program in Shanxi Province of China (No. 202103021224141), Teaching Reform Innovation Program for Higher Education Institutions in Shanxi Province of China (No. J202303045), and The Starting Fund for Doctoral Research of Shanxi Agricultural University (No. 2015YJ15 and No. 2015YJ16).
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G.R.M. is the executor of this research, completing data analysis and writing the first draft of the paper. H.Y.Z., L.Z.H., and X.Q. are the designers and principals of the project, guiding experimental design, data analysis, thesis writing, and modification. All authors read and agreed to the final text.
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Geng, R., Han, Y., Liu, Z. et al. The first genetic linkage map of sea buckthorn (Hippophae rhamnoides) and QTL mapping of growth-related traits. Hortic. Environ. Biotechnol. (2024). https://doi.org/10.1007/s13580-024-00597-4
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DOI: https://doi.org/10.1007/s13580-024-00597-4