Abstract
Despite substantial progress in clinical trials of osteoarthritis (OA) gene therapy, the prevalence of OA is still on the rise. MiRNAs have a potential biomarker and therapeutic target for OA. OA cartilage and chondrosarcoma cells were studied to determine the role of miR-29a-3p and PTEN. OA cartilage and human chondrosarcoma cells (SW1353) were obtained. miR-29a-3p and PTEN signature expression was determined by RT-qPCR. The binding relationship between miR-29a-3p and PTEN was investigated by dual-luciferase reporter gene and western blot assay. TUNEL, immunohistochemistry, CCK-8, and flow cytometry were utilized to determine the proliferation and apoptosis of SW1353 cells. This study indicated downregulation of miR-29a-3p expression and upregulation of PTEN expression in human OA primary chondrocytes or OA tissue samples, compared with the normal cartilage cells or tissues. PTEN expression was negatively correlated with miR-29a-3p expression, and miR-29a-3p targeted PTEN mechanistically. miR-29a-3p reduced SW1353 cell activity and proliferation and promoted cell apoptosis. However, the aforementioned effects could be reversed by downregulating PTEN. miR-29a-3p can stimulate chondrocyte proliferation and inhibit apoptosis by inhibiting PTEN expression.
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The data that support the findings of this study are available from the corresponding author, upon reasonable request.
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Conceptualization, Kai Zhu; methodology, Yan Zhang and DongDong Li; formal analysis, MingZhong Xie and HuaCai Jiang; investigation, KaiQuan Zhang and Yang Lei; data curation, Kai Zhu and GuangYou Chen; writing—original draft preparation, Kai Zhu; writing—review and editing, GuangYou Chen; project administration, Kai Zhu. All authors have read and agreed to the published version of the manuscript.
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All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All subjects were approved by The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University (No.20180325 cm).
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Zhu, K., Zhang, Y., Li, D. et al. MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression. Funct Integr Genomics 24, 54 (2024). https://doi.org/10.1007/s10142-024-01327-w
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DOI: https://doi.org/10.1007/s10142-024-01327-w