Abstract
REVEILLE (RVEs) transcription factors play an important role in regulating circadian clock, plant growth and development, and stress responses. While RVEs have been identified in various plant species, comprehensive genome exploration and analysis of RVEs in castor (Ricinus communis L.) have not been previously reported. In this study, we identified RcRVEs in the castor genome through sequence alignment and conducted an analysis encompassing gene structures, protein motifs, physicochemical properties, transcription patterns under cold stress, different tissues, and diurnal rhythm. A total of five RcRVEs were pinpointed in the castor genome. Evolutionary tree analysis categorized these RcRVEs into three distinct groups, implying that different RcRVEs may fulfill distinct biological functions. Furthermore, several abiotic stresses and hormone-responsive cis-acting elements were found in the promoters of the RcRVEs. RNA-sequencing (RNA-seq) analysis revealed that RcRVE1 and RcRVE7 were induced by cold stress and exhibited upregulation over time, indicating their involvement in regulating cold adaptation in castor plants. Five RcRVEs have notably higher expression levels in leaves compared to other tissues. The expression of all RcRVEs displayed diurnal rhythms under light/dark cycles. This study provides valuable insights into the potential functions of RcRVEs in regulating castor’s growth and development, and responses to cold stress.
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Acknowledgements
This study was funded by the National Natural Science Foundation of China (32360463; 32060493), the 22nd Batch of High-level Talent Development Funds of Inner Mongolia Autonomous Region, Entrepreneurship Startup Project for Overseas Scholar (Grant No. 2020QNCXRC07), Basic Research Project of Inner MongoliaAutonomous Region Directly-administered Universities (GXKY22056), The Program forYoung Talents of Science and Technology in the University of Inner Mongolia Autonomous Region (Grant No. NJYT-20-B25), and The Doctoral Program Fund of Inner Mongolia Minzu University (Grant No. BS398).
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XY Wang designed the study, X Wei, JX Zhang performed the research. XY Wang and X Wei wrote the paper. X Wei, XY Wang, MD Sun analyzed the data. N Wang, MD Sun, X Ding, H Xu, XM Yu, WR Yue, HY Huo and LL Yu modified the manuscript. All authors have read and approved the final manuscript.
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Wei, X., Zhang, J., Wang, N. et al. Genome-wide identification and analysis of expression pattern of REVEILLE transcription factors in castor (Ricinus communis L.). Hortic. Environ. Biotechnol. (2024). https://doi.org/10.1007/s13580-023-00580-5
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DOI: https://doi.org/10.1007/s13580-023-00580-5