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Validation of Daily Growth Increment Deposition in Sagittal Otoliths of the Young of the Year Lithognathus mormyrus

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Abstract

This paper aims to validate the micro increment deposition in the sagittal otoliths of young of the year (YOY) Lithognathus mormyrus in the shallow waters of Çanakkale, Turkey. Wild YOY specimens of L. mormyrus were captured and reared to validate the daily micro increment formation in sagittal otoliths. The formation of daily sagittal micro increments was validated using by alizarin Red S (ARS), at a concentration of 0 (control), 50 mg/lt, 100 mg/lt, 150 mg/lt, 200 mg/lt and 300 mg/lt. The natural photoperiod was applied, and no food was given throughout the marking period. The tanks kept strongly aerated to maintain the pH ~ 7. YOY fish were kept in different aquariums for 17 days post-dyeing and then re-dyeing as before and kept alive for a further 20 days. ARS marks were clearly observed in 71.7% of the YOY L. mormyrus sagittal otoliths under a fluorescent microscope. However, no mark could be observed in any of the samples of 50 mg/lt ARS treatment. Chi2 tests revealed that the linear regressions of the number of micro increments between ARS marks against number of elapsed times was not statistically significant. This result showed that micro increment deposition on the sagitta were laid down daily under the experimental conditions and can be used to detect the daily age information for YOY L. mormyrus.

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Funding

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, 118O321.

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Authors and Affiliations

  1. Marine Science and Technology Faculty, Department of Fisheries Industry Engineering, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Hakan Ayyıldız & Musa Bulut

  2. School of Graduate Studies, Department of Fishing and Processing Technology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Emre Kurtkaya

  3. Marine Science and Technology Faculty, Department of Aquaculture, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Pınar Çelik

  4. Faculty of Çanakkale Applied Sciences, Department of Fishery Technology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Aytaç Altın

Authors
  1. Hakan Ayyıldız
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  2. Emre Kurtkaya
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  3. Pınar Çelik
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  4. Aytaç Altın
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  5. Musa Bulut
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Contributions

Ayyildiz, H. wrote the main manuscript text and prepared figures. Kurtkaya, E, Altin, A. and Bulut, M. conducted field and laboratory studies. Çelik, P. conduct aquaculture process and all authors reviewed the manuscript.

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Correspondence to Hakan Ayyıldız.

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Ethical Approval

The care and use of experimental animals complied with [Çanakkale Onsekiz Mart University, Animal Experiments Local Ethics Committee] animal welfare laws, guidelines and policies as approved by [2018/02–10].

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Ayyıldız, H., Kurtkaya, E., Çelik, P. et al. Validation of Daily Growth Increment Deposition in Sagittal Otoliths of the Young of the Year Lithognathus mormyrus. Thalassas (2024). https://doi.org/10.1007/s41208-024-00686-2

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  • DOI: https://doi.org/10.1007/s41208-024-00686-2

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