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How host species and body part determine the microbial communities of five ambrosia beetle species

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Abstract

The ambrosia beetles are farming insects that feed mainly on their cultivated fungi, which in some occasions are pathogens from forest and fruit trees. We used a culture-independent approach based on 16S and 18S rRNA gene metabarcoding analysis to investigate the diversity and composition of the bacterial and fungal communities associated with five ambrosia beetle species: four species native to America (Monarthrum dimidiatum, Dryocoetoides capucinus, Euwallacea discretus, Corthylus consimilis) and an introduced species (Xylosandrus morigerus). For the bacterial community, the beetle species hosted a broad diversity with 1,579 amplicon sequence variants (ASVs) and 66 genera, while for the fungal community they hosted 288 ASVs and 39 genera. Some microbial groups dominated the community within a host species or a body part (Wolbachia in the head-thorax of E. discretus; Ambrosiella in the head-thorax and abdomen of X. morigerus). The taxonomic composition and structure of the microbial communities appeared to differ between beetle species; this was supported by beta-diversity analysis, which indicated that bacterial and fungal communities were clustered mainly by host species. This study characterizes for the first time the microbial communities associated with unexplored ambrosia beetle species, as well as the factors that affect the composition and taxonomic diversity per se, contributing to the knowledge of the ambrosia beetle system.

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Data availability

The 16S and 18S amplicon sequence data are available on NCBI (PRJNA1049028) and can be accessed at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1049028

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Acknowledgements

We would like to thank Ann Grant and Sean Rovito for her helpful discussions and suggestions. We also thank Guadalupe Hernández Cervantes for the processing of the biological samples and T. H. Atkinson for the photographs of the beetle specimens. We are grateful for the support of Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCyT) for a postdoctoral Fellowship to PSM (362331) and the Fondo Institucional de Fomento Regional para el Desarrollo Científico Tecnológico y de Innovación (FORDECyT), Grant No. 292399

Funding

This work was supported by Fondo Institucional de Fomento Regional para el Desarrollo Científico Tecnológico y de Innovación (FORDECyT) from Consejo Nacional de Ciencia y Tecnología (CONACyT), grant No. 292399.

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Authors and Affiliations

  1. Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Xalapa, C.P. 91070, México

    Paulette Calleros-González, Arturo Ibarra-Juarez & Araceli Lamelas

  2. Investigador Por México - CONAHCyT. Instituto de Ecología, A. C., Carretera Antigua a Coatepec 351, Xalapa, C.P. 91070, México

    Arturo Ibarra-Juarez

  3. Facultad de Química, Unidad de Química-Sisal, Universidad Nacional Autónoma de México, Sisal, Yucatán, 97356, México

    Pablo Suárez-Moo

Authors
  1. Paulette Calleros-González
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  2. Arturo Ibarra-Juarez
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Contributions

Araceli Lamelas and Arturo Ibarra Juarez designed the research. Arturo Ibarra-Juarez and Paulette Calleros-González contributed to experimental work. Paulette Calleros-González and Pablo Suárez-Moo performed the data analysis and wrote the manuscript. Pablo Suárez-Moo and Araceli Lamelas revised the manuscript. All authors reviewed and approved the manuscript.

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Correspondence to Araceli Lamelas or Pablo Suárez-Moo.

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Calleros-González, P., Ibarra-Juarez, A., Lamelas, A. et al. How host species and body part determine the microbial communities of five ambrosia beetle species. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00502-0

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