Abstract
Background and aims
Imbalances in nitrogen (N) and phosphorus (P) inputs in grasslands, induced by human activities, can significantly alter the C:N:P stoichiometry of plant communities and thereby affect ecosystem processes. However, in grasslands on karst, the effects of N and P inputs on aboveground C:N:P stoichiometry, species turnover, and plant nutrition remain unresolved.
Methods
We conducted a full factorial experiment with three levels of N and P fertilization levels to explore the effects of variable N and P fertilization on aboveground vegetation C:N:P stoichiometry. The C, N, and P concentrations were determined by elemental analysis and inductively coupled plasma − optical emission spectrometry.
Results
N and P fertilization significantly increased the vegetation N and P concentrations, while the C concentration remained stable or dropped. We observed reductions in the C:N, C:P, and N:P mass ratios, with the P addition impact being more pronounced than that of N. Interestingly, legumes and grasses, the dominant functional groups of plants, exhibited divergent stoichiometric reactions to nutrient additions. Grasses were more responsive, possibly because of greater C:N:P homeostatic flexibility and efficient nutrient acquisition. The alterations in community C:N:P stoichiometry were mainly driven by intragroup trait variation, especially in the dominant grass functional group, which responded most to increased soil N and P availability.
Conclusion
Our findings imply that the community stoichiometric alterations were driven by the grasses, the dominant plant functional group in the studied agriculturally improved pasture on karst.
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Data availability
The datasets generated and/or analyzed for the current study are available from the corresponding author on reasonable request.
References
Ball KR, Woodin SJ, Power SA, Brien C, Berger B, Smith P, Pendall E (2021) Integrating ecological stoichiometry to understand nutrient limitation and potential for competition in mixed pasture assemblages. J Soil Sci Plant Nutr 21:2489–2500. https://doi.org/10.1007/s42729-021-00539-4
Bitomský M, Kobrlová L, Hroneš M, Duchoslav M (2022) Plant functional groups and phylogenetic regularity control plant community bioelement composition through calcium and magnesium. Oikos 2023:e09546. https://doi.org/10.1111/oik.09546
Chen J, van Groenigen KJ, Hungate BA, Terrer C, van Groenigen JW, Maestre FT, Ying SC, Luo Y, Jorgensen U, Sinsabaugh RL, Olesen JE, Elsgaard L (2020) Long-term nitrogen loading alleviates phosphorus limitation in terrestrial ecosystems. Glob Change Biol 26:5077–5086. https://doi.org/10.1111/gcb.15218
Craine JM, Jackson RD (2010) Plant nitrogen and phosphorus limitation in 98 North American grassland soils. Plant Soil 334:73–84. https://doi.org/10.1007/s11104-009-0237-1
Deng M, Liu L, Sun Z, Piao S, Ma Y, Chen Y, Wang J, Qiao C, Wang X, Li P (2016) Increased phosphate uptake but not resorption alleviates phosphorus deficiency induced by nitrogen deposition in temperate Larix principis-rupprechtii plantations. New Phytol 212:1019–1029. https://doi.org/10.1111/nph.14083
Deng Q, Hui D, Dennis S, Reddy KC (2017) Responses of terrestrial ecosystem phosphorus cycling to nitrogen addition: A meta-analysis. Glob Ecol Biogeogr 26:713–728. https://doi.org/10.1111/geb.12576
Du E, Terrer C, Pellegrini AF, Ahlström A, van Lissa CJ, Zhao X, Xia N, Wu X, Jackson RB (2020) Global patterns of terrestrial nitrogen and phosphorus limitation. Nat Geosci 13:221–226. https://doi.org/10.1038/s41561-019-0530-4
Elser JJ, Fagan WF, Kerkhoff AJ, Swenson NG, Enquist BJ (2010) Biological stoichiometry of plant production: metabolism, scaling and ecological response to global change. New Phytol 186:593–608. https://doi.org/10.1111/j.1469-8137.2010.03214.x
Fang Z, Li D-D, Jiao F, Yao J, Du H-T (2019) The latitudinal patterns of leaf and soil C: N: P stoichiometry in the loess plateau of China. Front Plant Sci 10:85. https://doi.org/10.3389/fpls.2019.00085
Garnier E, Cortez J, Billès G, Navas M-L, Roumet C, Debussche M, Laurent G, Blanchard A, Aubry D, Bellmann A (2004) Plant functional markers capture ecosystem properties during secondary succession. Ecology 85:2630–2637. https://doi.org/10.1890/03-0799
Grimoldi AA, Kavanová M, Lattanzi FA, Schnyder H (2005) Phosphorus nutrition-mediated effects of arbuscular mycorrhiza on leaf morphology and carbon allocation in perennial ryegrass. New Phytol 168:435–444. https://doi.org/10.1111/j.1469-8137.2005.01500.x
Guo Y, Yang X, Schöb C, Jiang Y, Tang Z (2017) Legume shrubs are more nitrogen-homeostatic than non-legume shrubs. Front Plant Sci 8:1662. https://doi.org/10.3389/fpls.2017.01662
Heyburn J, McKenzie P, Crawley MJ, Fornara DA (2017) Effects of grassland management on plant C:N: P stoichiometry: implications for soil element cycling and storage. Ecosphere 8:e01963. https://doi.org/10.1002/ecs2.1963
Hillebrand H, Cowles JM, Lewandowska A, Van de Waal DB, Plum C (2014) Think ratio! A stoichiometric view on biodiversity–ecosystem functioning research. Basic Appl Ecol 15:465–474. https://doi.org/10.1016/j.baae.2014.06.003
Houlton BZ, Wang Y-P, Vitousek PM, Field CB (2008) A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature 454:327–330. https://doi.org/10.1038/nature07028
Hu Y-Y, Wei H-W, Zhang Z-W, Hou S-L, Yang J-J, Wang J-F, Lü X-T (2020) Changes of plant community composition instead of soil nutrient status drive the legacy effects of historical nitrogen deposition on plant community N: P stoichiometry. Plant Soil 453:503–513. https://doi.org/10.1007/s11104-020-04631-z
Jiang Z, Lian Y, Qin X (2014) Rocky desertification in Southwest China: Impacts, causes, and restoration. Earth Sci Rev 132:1–12. https://doi.org/10.1016/j.earscirev.2014.01.005
Jiang J, Wang Y-P, Liu F, Du Y, Zhuang W, Chang Z, Yu M, Yan J (2021) Antagonistic and additive interactions dominate the responses of belowground carbon-cycling processes to nitrogen and phosphorus additions. Soil Biol Biochem 156:108216. https://doi.org/10.1016/j.soilbio.2021.108216
Kaspari M, de Beurs KM, Welti EA (2021) How and why plant ionomes vary across North American grasslands and its implications for herbivore abundance. Ecology 102:e03459. https://doi.org/10.1002/ecy.3459
Lepš J, de Bello F, Šmilauer P, Doležal J (2011) Community trait response to environment: disentangling species turnover vs intraspecific trait variability effects. Ecography 34:856–863. https://doi.org/10.1111/j.1600-0587.2010.06904.x
Li S, Wang F, Chen M, Liu Z, Zhou L, Deng J, Dong C, Bao G, Bai T, Li Z (2020) Mowing alters nitrogen effects on the community-level plant stoichiometry through shifting plant functional groups in a semi-arid grassland. Environ Res Lett 15:074031. https://doi.org/10.1088/1748-9326/ab8a87
Li Z, Qiu X, Sun Y, Liu S, Hu H, Xie J, Chen G, Xiao Y, Tang Y, Tu L (2021) C:N: P stoichiometry responses to 10 years of nitrogen addition differ across soil components and plant organs in a subtropical Pleioblastus amarus forest. Sci Total Environ 796:148925. https://doi.org/10.1016/j.scitotenv.2021.148925
Li W, Gan X, Jiang Y, Cao F, Lu XT, Ceulemans T, Zhao C (2022) Nitrogen effects on grassland biomass production and biodiversity are stronger than those of phosphorus. Environ Pollut 309:119720. https://doi.org/10.1016/j.envpol.2022.119720
Liang XS, Ma W, Hu JX, Zhang BC, Wang ZW, Lu XT (2022) Extreme drought exacerbates plant nitrogen-phosphorus imbalance in nitrogen enriched grassland. Sci Total Environ 849:157916. https://doi.org/10.1016/j.scitotenv.2022.157916
Liu C, Liu Y, Guo K, Wang S, Yang Y (2014) Concentrations and resorption patterns of 13 nutrients in different plant functional types in the karst region of south-western China. Ann Bot 113:873–885. https://doi.org/10.1093/aob/mcu005
Liu C, Liu Y, Guo K, Qiao X, Zhao H, Wang S, Zhang L, Cai X (2018) Effects of nitrogen, phosphorus and potassium addition on the productivity of a karst grassland: Plant functional group and community perspectives. Ecol Eng 117:84–95. https://doi.org/10.1016/j.ecoleng.2018.04.008
Liu M, Shen Y, Li Q, Xiao W, Song X (2021) Arbuscular mycorrhizal fungal colonization and soil pH induced by nitrogen and phosphorus additions affects leaf C: N: P stoichiometry in Chinese fir (Cunninghamia lanceolata) forests. Plant Soil 461:421–440. https://doi.org/10.1007/s11104-021-04831-1
Liu K, Liu Z, Zhou N et al (2022) Predicted increased P relative to N growth limitation of dry grasslands under soil acidification and alkalinization is ameliorated by increased precipitation. Soil Biol Biochem 173:108812. https://doi.org/10.1016/j.soilbio.2022.108812
Lü XT, Reed S, Yu Q, He NP, Wang ZW, Han XG (2013) Convergent responses of nitrogen and phosphorus resorption to nitrogen inputs in a semiarid grassland. Glob Change Biol 19:2775–2784. https://doi.org/10.1111/gcb.12235
Luo X, Mazer SJ, Guo H, Zhang N, Weiner J, Hu S (2016) Nitrogen: phosphorous supply ratio and allometry in five alpine plant species. Ecol Evol 6:8881–8892. https://doi.org/10.1002/ece3.2587
Mao R, Chen HM, Zhang XH, Shi FX, Song CC (2016) Effects of P addition on plant C:N: P stoichiometry in an N-limited temperate wetland of Northeast China. Sci Total Environ 559:1–6. https://doi.org/10.1016/j.scitotenv.2016.03.158
Mendoza R, García I, Depalma D, López CF (2016) Competition and growth of a grass–legume mixture fertilised with nitrogen and phosphorus: effect on nutrient acquisition, root morphology and symbiosis with soil microorganisms. Crop Pasture Sci 67:629–640. https://doi.org/10.1071/CP1525
Ning Z, Zhao X, Li Y, Wang L, Lian J, Yang H, Li Y (2021) Plant community C:N: P stoichiometry is mediated by soil nutrients and plant functional groups during grassland desertification. Ecol Eng 162:106179. https://doi.org/10.1016/j.ecoleng.2021.106179
Novotny AM, Schade JD, Hobbie SE, Kay AD, Kyle M, Reich PB, Elser JJ (2007) Stoichiometric response of nitrogen-fixing and non-fixing dicots to manipulations of CO2, nitrogen, and diversity. Oecologia 151:687–696. https://doi.org/10.1007/s00442-006-0599-5
Paseka RE, Bratt AR, MacNeill KL, Burian A, See CR (2019) Elemental ratios link environmental change and human health. Front Ecol Evol 7:1–12. https://doi.org/10.3389/fevo.2019.00378
Peng J, Ma F, Tian D, Quan Q, Chen C, Liao J, Zhou Q, Niu S (2023) Plant C: N ratio predicts community composition changes under N addition in an alpine meadow. J Ecol 111:589–600. https://doi.org/10.1111/1365-2745.14048
Peñuelas J, Sardans J (2022) The global nitrogen-phosphorus imbalance. Science 375:266–267. https://doi.org/10.1126/science.abl4827
Peñuelas J, Sardans J, Rivas-ubach A, Janssens IA (2012) The human-induced imbalance between C, N and P in Earth’s life system. Glob Change Biol 18:3–6. https://doi.org/10.1111/j.1365-2486.2011.02568.x
Penuelas J, Janssens IA, Ciais P, Obersteiner M, Sardans J (2020) Anthropogenic global shifts in biospheric N and P concentrations and ratios and their impacts on biodiversity, ecosystem productivity, food security, and human health. Glob Change Biol 26:1962–1985. https://doi.org/10.1111/gcb.14981
Pirhofer-Walzl K, Søegaard K, Høgh-Jensen H, Eriksen J, Sanderson MA, Rasmussen J, Rasmussen J (2011) Forage herbs improve mineral composition of grassland herbage. Grass Forage Sci 66(3):415–423. https://doi.org/10.1111/j.1365-2494.2011.00799.x
Smith MD, Knapp AK, Collins SL (2009) A framework for assessing ecosystem dynamics in response to chronic resource alterations induced by global change. Ecology 90:3279–3289. https://doi.org/10.1890/08-1815.1
Sun Y, Wang C, Chen X, Liu S, Lu X, Chen HYH, Ruan H (2022) Phosphorus additions imbalance terrestrial ecosystem C:N: P stoichiometry. Glob Change Biol 28:7353–7365. https://doi.org/10.1111/gcb.16417
Tang C, Weligama C, Sale P (2013) Subsurface soil acidification in farming systems: its possible causes and management options. In: Xu J (ed) Molecular environmental soil science, 3 Springer Netherlands, Dordrecht, pp 389–412. https://doi.org/10.1007/978-94-007-4177-5_13
Tao Y, Zhou X-B, Li Y-G, Liu H-L, Zhang Y-M (2022) Short-term N and P additions differentially alter the multiple functional traits and trait associations of a desert ephemeral plant in China. Environ Exp Bot 200. https://doi.org/10.1016/j.envexpbot.2022.104932
Tian Q, Lu P, Zhai X, Zhang R, Zheng Y, Wang H, Nie B, Bai W, Niu S, Shi P (2022) An integrated belowground trait-based understanding of nitrogen-driven plant diversity loss. Glob Change Biol 28:3651–3664. https://doi.org/10.1111/gcb.16147
Unger S, Habermann FM, Schenke K, Jongen M (2021) Arbuscular mycorrhizal fungi and nutrition determine the outcome of competition between Lolium multiflorum and Trifolium subterraneum. Front Plant Sci 12:778861. https://doi.org/10.3389/fpls.2021.778861
Valentine AJ, Kleinert A, Benedito VA (2017) Adaptive strategies for nitrogen metabolism in phosphate deficient legume nodules. Plant Sci 256:46–52. https://doi.org/10.1016/j.plantsci.2016.12.010
Vázquez E, Schleuss PM, Borer ET, Bugalho MN, Caldeira MC, Eisenhauer N, ... Spohn M (2022) Nitrogen but not phosphorus addition affects symbiotic N2 fixation by legumes in natural and semi-natural grasslands located on four continents. Plant Soil 478:689–707. https://doi.org/10.1007/s11104-022-05498-y
Vitousek PM, Porder S, Houlton BZ, Chadwick OA (2010) Terrestrial phosphorus limitation: mechanisms, implications, and nitrogen–phosphorus interactions. Ecol Appl 20:5–15. https://doi.org/10.1890/08-0127.1
Wang R, Balkanski Y, Boucher O, Ciais P, Peñuelas J, Tao S (2015) Significant contribution of combustion-related emissions to the atmospheric phosphorus budget. Nat Geosci 8:48–54. https://doi.org/10.1038/ngeo2324
Wang HY, Wang ZW, Ding R, Hou SL, Yang GJ, Lu XT, Han XG (2018) The impacts of nitrogen deposition on community N: P stoichiometry do not depend on phosphorus availability in a temperate meadow steppe. Environ Pollut 242:82–89. https://doi.org/10.1016/j.envpol.2018.06.088
Wang X, Yan X, Huang K, Luo X, Zhang Y, Zhou L, Yang F, Xu X, Zhou X, Niu K (2022) Nitrogen enrichment and warming shift community functional composition via distinct mechanisms: The role of intraspecific trait variability and species turnover. Funct Ecol 36:1230–1242. https://doi.org/10.1111/1365-2435.14012
Xia S, Jiang J, Liu F, Chang Z, Yu M, Liu C, Wang Y-P, Yan J (2023) Phosphorus addition promotes plant nitrogen uptake mainly via enhancing microbial activities: A global meta-analysis. Appl Soil Ecol 188:104927. https://doi.org/10.1016/j.apsoil.2023.104927
Xiao D, Xiao L, Che R, Tan Y, Liu X, Yang R, Zhang W, He X, Wang K (2020) Phosphorus but not nitrogen addition significantly changes diazotroph diversity and community composition in typical karst grassland soil. Agr Ecosyst Environ 301:106987. https://doi.org/10.1016/j.agee.2020.106987
Xu H, Qu Q, Li G, Liu G, Geissen V, Ritsema CJ, Xue S (2022) Impact of nitrogen addition on plant-soil-enzyme C-N–P stoichiometry and microbial nutrient limitation. Soil Biol Biochem 170:108714. https://doi.org/10.1016/j.soilbio.2022.108714
Yan Z, Kim N, Han W, Guo Y, Han T, Du E, Fang J (2014) Effects of nitrogen and phosphorus supply on growth rate, leaf stoichiometry, and nutrient resorption of Arabidopsis thaliana. Plant Soil 388:147–155. https://doi.org/10.1007/s11104-014-2316-1
Yan Z, Hou X, Han W, Ma S, Shen H, Guo Y, Fang J (2018) Effects of nitrogen and phosphorus supply on stoichiometry of six elements in leaves of Arabidopsis thaliana. Ann Bot 123:441–450. https://doi.org/10.1093/aob/mcy169
Yang D, Song L, Jin G (2019) The soil C: N: P stoichiometry is more sensitive than the leaf C: N: P stoichiometry to nitrogen addition: a four-year nitrogen addition experiment in a Pinus koraiensis plantation. Plant Soil 442:183–198. https://doi.org/10.1007/s11104-019-04165-z
Yang S, Liu W, Guo L, Wang C, Deng M, Peng Z, Liu L (2022) The changes in plant and soil C pools and their C: N stoichiometry control grassland N retention under elevated N inputs. Ecol Appl 32:e2517. https://doi.org/10.1002/eap.2517
You C, Wu F, Yang W, Xu Z, Tan B, Yue K, Ni X (2018) Nutrient-limited conditions determine the responses of foliar nitrogen and phosphorus stoichiometry to nitrogen addition: A global meta-analysis. Environ Pollut 241:740–749. https://doi.org/10.1016/j.envpol.2018.06.018
Yuan ZY, Chen HYH (2015) Decoupling of nitrogen and phosphorus in terrestrial plants associated with global changes. Nat Clim Chang 5:465–469. https://doi.org/10.1038/nclimate2549
Zhan S, Wang Y, Zhu Z, Li W, Bai Y (2017) Nitrogen enrichment alters plant N: P stoichiometry and intensifies phosphorus limitation in a steppe ecosystem. Environ Exp Bot 134:21–32. https://doi.org/10.1016/j.envexpbot.2016.10.014
Zhang J, Yan X, Su F, Li Z, Wang Y, Wei Y, Ji Y, Yang Y, Zhou X, Guo H, Hu S (2018) Long-term N and P additions alter the scaling of plant nitrogen to phosphorus in a Tibetan alpine meadow. Sci Total Environ 625:440–448. https://doi.org/10.1016/j.scitotenv.2017.12.292
Zhao Y, Yang B, Li M, Xiao R, Rao K, Wang J, Zhang T, Guo J (2019) Community composition, structure and productivity in response to nitrogen and phosphorus additions in a temperate meadow. Sci Total Environ 654:863–871. https://doi.org/10.1016/j.scitotenv.2018.11.155
Zheng Z, Lu J, Su Y, Yang Q, Lin Y, Liu H, Yang J, Huang H, Wang X (2020) Differential effects of N and P additions on foliar stoichiometry between species and community levels in a subtropical forest in eastern China. Ecol Ind 117:106537. https://doi.org/10.1016/j.ecolind.2020.106537
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We thank the editor and reviewers for their useful insights and constructive comments.
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This work was supported by the Guizhou Provincial Science and Technology Projects (Qian Ke He [2022] Yi Ban 040 and QKHPTRC-CXTD [2022] 011), the Guizhou Education Cooperation Projects (Qianjiaoji [2022] 120), and the Guizhou University Projects (Gui Da Ji Chu [2023] 13 and Gui Da Ren Ji He [2022] 70).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Jing Zhou, Fugui Yang and Jin Ye. The first draft of the manuscript was written by Jin Ye and Jihui Chen, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ye, J., Zhou, J., Yang, F. et al. Alternations in the element stoichiometry of the grasses drive the aboveground C:N:P ratio of an agriculturally improved pasture on karst in response to differential N and P fertilization. Plant Soil (2024). https://doi.org/10.1007/s11104-024-06621-x
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DOI: https://doi.org/10.1007/s11104-024-06621-x