Abstract
Sorghum is an important food crop commonly used for brewing, feed, and bioenergy. Certain genotypes of sorghum contain high concentrations of condensed tannins in seeds, which are beneficial, such as protecting grains from herbivore bird pests, but also impair grain quality and digestibility. Previously, we identified Tannin1 and Tannin2, each with three recessive causal alleles, regulate tannin absence in sorghum. In this study, via characterizing 421 sorghum accessions, we further identified three novel recessive alleles from these two genes. The tan1-d allele contains a 12-bp deletion at position 659 nt and the tan1-e allele contains a 10-bp deletion at position 771 nt in Tannin1. The tan2-d allele contains a C-to-T transition, which results in a premature stop codon before the bHLH domain in Tannin2, and was predominantly selected in China. We further developed KASP assays targeting these identified recessive alleles to efficiently genotype large populations. These studies provide new insights in sorghum domestication and convenient tools for breeding programs.
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Data Availability
All datasets generated or analyzed during this study are included in this published article and its supplementary information files. Materials used in this study are available from the corresponding authors.
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Acknowledgements
We thank Dr. Qingjun Cheng and Dr. Ping Lu for assistance with sorghum seed.
Funding
This work is funded by the Shandong Province Agricultural Fine Seeds Project (2021LZGC006) and National Science Foundation of China (31871695).
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YW, XL and SL conceived of the research. WZ, XZ, YW, XL, RB, and SL wrote the manuscript; WZ, XZ, CZ, YZ, YX, and YW performed the experiments; HZ and HW provided sorghum germplasm and analyzed the data; all authors revised and approved the final version of the manuscript.
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Zhang, W., Benke, R., Zhang, X. et al. Novel allelic variations in Tannin1 and Tannin2 contribute to tannin absence in sorghum. Mol Breeding 44, 24 (2024). https://doi.org/10.1007/s11032-024-01463-y
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DOI: https://doi.org/10.1007/s11032-024-01463-y