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Understanding the Angiogenic Characteristics of Clinical-Grade Mesenchymal Stromal Cells Isolated from Human Umbilical Cord

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Abstract

Addressing the challenges in managing ischemic tissue repair and remodelling remains a prominent clinical concern. Current research is heavily concentrated on identifying innovative cell-based therapies with the potential to enhance revascularization in patients affected by these diseases. We have previously developed and validated a manufacturing process for human umbilical cord mesenchymal stromal cells (UC-MSCs)-based cell therapy medicinal product, according to Good Manufacturing Practices. In this study, we demonstrate that these UC-MSCs enhance the proliferation and migration of endothelial cells and the formation of capillary structures. Moreover, UC-MSCs and endothelial cells interact, allowing UC-MSCs to acquire a perivascular cell phenotype and consequently provide direct support to the newly formed vascular network. This characterization of the proangiogenic properties of this UC-MSCs based-cell therapy medicinal product is an essential step for its therapeutic assessment in the clinical context of vascular regeneration.

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Fig. 1

Data Availability

Raw data are available upon request.

References

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Funding

This work was supported by AP-HP, University Paris Cité and Inserm.

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Authors and Affiliations

  1. Université de Paris Cité, INSERM, Innovative Therapies in Hemostasis, F-75006, Paris, France

    Charlotte Carcopino, Elisa Rossi, Divina El Hamaoui, Pascale Gaussem, David M. Smadja & Audrey Cras

  2. Centre d’Investigation Clinique en Biothérapies CBT501, INSERM, F-75010, Paris, France

    Charlotte Carcopino, Miryam Mebarki, Jérôme Larghero & Audrey Cras

  3. Cell therapy Department, AP-HP, Saint Louis Hospital, F-75010, Paris, France

    Miryam Mebarki, Jérôme Larghero & Audrey Cras

  4. Université de Paris Cité, INSERM, U976, F-75010, Paris, France

    Miryam Mebarki & Jérôme Larghero

  5. Hematology Department, AP-HP, Georges Pompidou European Hospital, 20 rue Leblanc, F-75015, Paris, France

    Pascale Gaussem & David M. Smadja

Authors
  1. Charlotte Carcopino
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  2. Elisa Rossi
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  3. Miryam Mebarki
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  4. Divina El Hamaoui
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  5. Pascale Gaussem
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  6. Jérôme Larghero
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  7. David M. Smadja
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  8. Audrey Cras
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Contributions

AC, ER and DMS designed the study. CC, MM and DEH performed the experiments and analyzed data. AC, ER JL, PG and DMS interpreted and discussed results. AC and DMS wrote the paper. Authors declare that the submitted work is original and has not been published before and that the work is not under consideration for publication elsewhere.

Corresponding author

Correspondence to David M. Smadja.

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Ethical Approval

Human umbilical cord were provided by Saint-Louis Hospital Biological Resources Center—Cord Blood Bank after information and consent of mothers, under French Health Ministry authorization (n°AC-2022-5325).

Competing Interests

Authors declare no conflict of interest related to this work.

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Carcopino, C., Rossi, E., Mebarki, M. et al. Understanding the Angiogenic Characteristics of Clinical-Grade Mesenchymal Stromal Cells Isolated from Human Umbilical Cord. Stem Cell Rev and Rep (2024). https://doi.org/10.1007/s12015-024-10712-8

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  • DOI: https://doi.org/10.1007/s12015-024-10712-8

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