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Isolation of phages against Streptococcus species in the oral cavity for potential control of dental diseases and associated systemic complications

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Abstract

Dental infections and systemic complications caused by Streptococcus species in the oral cavity are increasingly exhibiting resistance to commonly used antibiotics, posing a potential threat to global public health. Phage therapy may offer a superior alternative, given that bacteriophages can be easily isolated and rapidly replicate in large numbers. In this study, six Streptococcus species from the oral cavity were characterized. Bacteriophages isolated from wastewater using five of these species as hosts produced plaques ranging from 0.2 to 2.4 mm in size. The phages demonstrated stability within a temperature range of 4 ℃ to 37 ℃. However, at temperatures exceeding 45 ℃, a noticeable reduction in bacteriophage titer was observed. Similarly, the phages showed greater stability within a pH range of 5 to 10. The isolated phages exhibited latency periods ranging from 15 to 20 min and had burst sizes varying from 10 to 200 viral particles. This study supports the potential use of bacteriophages in controlling infections caused by Streptococcus species.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya

    Beatrice Chepchumba & George. O. Asudi

  2. Center for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya

    Japhet Katana & Musa. O. Ngayo

  3. Department of Zoology, Jomo Kenyatta University of Science and Technology, Juja, Kenya

    Juliah Akhwale Khayeli

Authors
  1. Beatrice Chepchumba
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  2. George. O. Asudi
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  3. Japhet Katana
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  4. Musa. O. Ngayo
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  5. Juliah Akhwale Khayeli
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Contributions

All authors contributed to the study's conception and design. Beatrice Chepchumba performed material preparation, data collection and analysis. Beatrice Chepchumba wrote the first draft of the manuscript, and all authors commented on previous versions. All authors read and approved the final manuscript.

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Correspondence to Beatrice Chepchumba.

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Ethical approval

The study was approved by the Kenyatta University Ethical committee. Research approval was sought from the National commission for science and Technology (NACOSTI)

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Communicated by Ran Wang.

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Chepchumba, B., Asudi, G.O., Katana, J. et al. Isolation of phages against Streptococcus species in the oral cavity for potential control of dental diseases and associated systemic complications. Arch Microbiol 206, 175 (2024). https://doi.org/10.1007/s00203-024-03897-6

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  • DOI: https://doi.org/10.1007/s00203-024-03897-6

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