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Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model

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Abstract

The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to play roles in both physiological and pathological processes. The cadherin-based adherent junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain somewhat ambiguous. This research utilized in situ hybridization (ISH) to examine the expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile plaques in the AD mouse brain were also bound to these probes. This binding, however, did not exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our data suggest that although antisense cRNA probes effectively detected cadherin mRNA expression in AD brain cells, their association with senile plaques may not specifically indicate cadherin mRNA expression.

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ACKNOWLEDGMENTS

This work was supported by the Anatomy I Institute of the University of Jena. The author thanks Dr. Kaether, Prof. Dr. Jucker, and Prof. Dr. Haass and their groups for the mouse model and antibodies and Dr. F. van Roy, Dr. M. Takeichi, Dr. T. Jessell and Dr. H. Cremer for some plasmids. All authors participated in this study and have access to all data in this work.

Funding

This study is supported by the Scientific and Technological Research Project of Henan Provincial Department of Science and Technology (Grant no. 212102310217) and the grant from Funds of the National Natural Science Foundation of China (Grant no. 81401015).

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Authors and Affiliations

  1. School of Basic Medicine, Zhengzhou University, 450001, Zhengzhou, China

    H. Zhou, S. J. Du, H. Y. Li, J. Fu & C. Chang

  2. Department of General and Visceral Surgery, Goethe University Hospital, 60596, Frankfurt am Main, Germany

    H. Zhou

  3. Uganda Christian University, P.O. Box 4 10120, Mukono, Uganda

    F. Gendi

  4. Center of Cerebral Palsy Surgical Research and Treatment, Zhengzhou University, 450001, Zhengzhou, China

    C. Chang

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  1. H. Zhou
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  2. S. J. Du
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  3. F. Gendi
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  4. H. Y. Li
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Contributions

H.Z. conceived of the study and designed the study; H.Z. and S.D. collected, analyzed the data, and wrote the initial draft of the paper; C.C. was responsible for the project administration and supervision; all authors contributed by carrying out additional analyses and revising the manuscript.

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Correspondence to C. Chang.

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All studies were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and its later amendments. They were also approved by the Zhengzhou University (Zhengzhou, Henan, China), protocol no. 2021-265 dated January 6, 2021.

Each participant in the study provided a voluntary written informed consent after receiving an explanation of the potential risks and benefits, as well as the nature of the upcoming study.

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Zhou, H., Du, S.J., Gendi, F. et al. Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model. Mol Biol (2024). https://doi.org/10.1134/S0026893324700134

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