Correction to: BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells

Correction to: Cell Tissue Res (2016) 366:101–11 https://doi.org/10.1007/s00441-016-2403-0

The authors regret that the versions of Figs. 3, 4 and 5 that appears in the original published article are incorrect.

Below are the incorrect figures:

Fig. 3

BMP2-induced expression of various types of collagens within the same system. a, b, b’ Type I collagen (Col1A1) and type X collagen (Col10A1) protein levels weremeasured after 5, 7 and 9 days by using the Western blot method; BMP2 induced both Col1A1 and Col10A1 protein expression. Data are denoted as means ± SD. *P < 0.05, vs. Ad-BMP2. c–c’’ Col1A protein level was measured after 9 days by immunohistochemical analysis. d–d’’ Type II collagen (Col2A1) protein level was measured after 9 days by immunohistochemical analysis. e–e’’ Col10A1 protein level was measured after 9 days by immunohistochemical analysis. Bars 50 μm

Fig. 4

Staining used to identify chondrogenic and osteogenic differentiation. a–a’’, b–b’’ Glycosaminoglycan (GAG) levels were investigated by using Alcian blue staining. BMP2 stimulated the secretion of sulfated GAGs after 7 and 9 days. c–c’’, d–d’’ Alkaline phosphatase (ALP) staining was also conducted on days 7 and 9. The staining results indicated that BMP2 increased ALP expression. e–e’’, f–f’’ Alizarin red S staining was conducted 7 and 9 days after infection; BMP2 increased the number of calcium mineral deposits. Bars 50 μm. g ALP chemiluminescence assay was conducted 7 and 9 days after infection. *P < 0.05, vs. Ad-BMP2

Fig. 5

BMP2-induced osteochondrogenic differentiation in vivo. a Macrographic images of ectopic bone masses retrieved after 5 weeks. b–e’ Histological evaluations of retrieved bone masses; the bone masses retrieved from the BMP2-induced groups were fixed and decalcified. b, b’ Hematoxylin and eosin (H&E) staining. c, c’ Masson’s trichrome staining. d, d’ Alcian blue staining. e, e’ Safranin O-fast green staining. Bars 50 μm

The correct Figs. 3, 4, and 5 appears below:

Fig. 3

BMP2-induced expression of various types of collagens within the same system. a, b, b’ Type I collagen (Col1A1) and type X collagen (Col10A1) protein levels weremeasured after 5, 7 and 9 days by using the Western blot method; BMP2 induced both Col1A1 and Col10A1 protein expression. Data are denoted as means ± SD. *P < 0.05, vs. Ad-BMP2. c–c’’ Col1A protein level was measured after 9 days by immunohistochemical analysis. d–d’’ Type II collagen (Col2A1) protein level was measured after 9 days by immunohistochemical analysis. e–e’’ Col10A1 protein level was measured after 9 days by immunohistochemical analysis. Bars 50 μm

Fig. 4

Staining used to identify chondrogenic and osteogenic differentiation. a–a’’, b–b’’ Glycosaminoglycan (GAG) levels were investigated by using Alcian blue staining. BMP2 stimulated the secretion of sulfated GAGs after 7 and 9 days. c–c’’, d–d’’ Alkaline phosphatase (ALP) staining was also conducted on days 7 and 9. The staining results indicated that BMP2 increased ALP expression. e–e’’, f–f’’ Alizarin red S staining was conducted 7 and 9 days after infection; BMP2 increased the number of calcium mineral deposits. Bars 50 μm. g ALP chemiluminescence assay was conducted 7 and 9 days after infection. *P < 0.05, vs. Ad-BMP2

Fig. 5

BMP2-induced osteochondrogenic differentiation in vivo. a Macrographic images of ectopic bone masses retrieved after 5 weeks. b–e’ Histological evaluations of retrieved bone masses; the bone masses retrieved from the BMP2-induced groups were fixed and decalcified. b, b’ Hematoxylin and eosin (H&E) staining. c, c’ Masson’s trichrome staining. d, d’ Alcian blue staining. e, e’ Safranin O-fast green staining. Bars 50 μm

The original article has been corrected.