Impacts of cationic lipid–DNA complexes on immune cells and hematopoietic cells in vivo

Xiuxiu Cong; Huizhu Tan; Yue Lv; Kuirong Mao; Yanbao Xin; Jialiang Wang; Xiandi Meng; Meng Guan; Haorui Wang; Yong-Guang Yang; Tianmeng Sun

doi:10.1039/D4BM00148F

Impacts of cationic lipid–DNA complexes on immune cells and hematopoietic cells in vivo†

Xiuxiu Cong,^ab Huizhu Tan,^ab Yue Lv,^ab Kuirong Mao,^abc Yanbao Xin,^ab Jialiang Wang,^e Xiandi Meng,^ab Meng Guan,^a Haorui Wang,^abc Yong-Guang Yang^abc and Tianmeng Sun

*^abcd

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* Corresponding authors

^a Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, The First Hospital, Jilin University, Changchun, Jilin, China
E-mail: tsun41@jlu.edu.cn

^b National-local Joint Engineering Laboratory of Animal Models for Human Diseases, Changchun, Jilin, China

^c International Center of Future Science, Jilin University, Changchun, Jilin, China

^d State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, Jilin, China

^e Department of Nuclear Medicine, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China

Abstract

The inability to systemic administration of nanoparticles, particularly cationic nanoparticles, has been a significant barrier to their clinical translation due to toxicity concerns. Understanding the in vivo behavior of cationic lipids is crucial, given their potential impact on critical biological components such as immune cells and hematopoietic stem cells (HSC). These cells are essential for maintaining the body's homeostasis, and their interaction with cationic lipids is a key factor in determining the safety and efficacy of these nanoparticles. In this study, we focused on the cytotoxic effects of cationic lipid/DNA complexes (CLN/DNA). Significantly, we observed that the most substantial cytotoxic effects, including a marked increase in numbers of long-term hematopoietic stem cells (LT-HSC), occurred 24 h post-CLN/DNA treatment in mice. Furthermore, we found that CLN/DNA-induced HSC expansion in bone marrow (BM) led to a notable decrease in the ability to reestablish blood cell production. Our study provides crucial insights into the interaction between cationic lipids and vital cellular components of the immune and hematopoietic systems.

Biomaterials Science

Impacts of cationic lipid–DNA complexes on immune cells and hematopoietic cells in vivo†

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Impacts of cationic lipid–DNA complexes on immune cells and hematopoietic cells in vivo

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