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The Antioxidant Activity and the Protective Effect of Rosa canina L. Fruit Against Intestinal Inflammation Induced by Hyperhomocysteinemia in Mice

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Pharmaceutical Chemistry Journal Aims and scope

Inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, are immune-mediated chronic inflammatory diseases of the intestinal tract. Under conditions of low vitamin B content, homocysteine accumulates and leads to hyperhomocysteinemia. The association between hyperhomocysteinemia and the risk of IBD has been widely studied. In the current study, Rosa canina L. fruit (RC) extract was analyzed by GC–MS, which revealed the presence of n-hexadecanoic acid, oleic acid, and stearic acid, which were identified as major compounds. The total phenolic and flavonoid contents of the RC extract were assessed. Additionally, the antioxidant activity of RC extract was checked using several radical scavenging activities, furthermore, a step-down method was used to confirm the nontoxicity of RC. The protective effect of RC on mice intestinal inflammation (induced by hyperhomocysteinemia) was evaluated according to an experimental in vivo procedure of 21 days. The level of plasma inflammatory markers (hs-CRP, homocysteine), and the reduced glutathione (GSH) content of liver tissue were measured. Additionally, the histological sections of the intestinal tissue were examined. Our results revealed a potent antioxidant capacity of RC extract. On the other hand, RC considerably reduced the histopathological changes, increased the level of GSH, and restored the integrity of the intestinal epithelium. Therefore, we recommend the use of RC as a natural anti hyperhomocysteinemia and antiinflammatory therapeutic agent.

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Authors and Affiliations

  1. Université des Frères Mentouri-Constantine 1, Département de Biologie Animale, Faculté des Sciences de la Nature et de la Vie, Constantine, Algeria

    Sara Khelfi, Sakina Zerizer & Souraya Tebibel

  2. Université des Frères Mentouri-Constantine 1, Laboratoired’ObtentiondeSubstances Thérapeutiques, 25000, Constantine, Algeria

    Sara Khelfi, Sakina Zerizer & Zahia Kabouche

  3. Center of Biotechnology Research (CRBt), Ali Mendjli UV 03 BP E73, 25000, Constantine, Algeria

    Chawki Bensouici

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  1. Sara Khelfi
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Khelfi, S., Zerizer, S., Bensouici, C. et al. The Antioxidant Activity and the Protective Effect of Rosa canina L. Fruit Against Intestinal Inflammation Induced by Hyperhomocysteinemia in Mice. Pharm Chem J 57, 1778–1788 (2024). https://doi.org/10.1007/s11094-024-03079-0

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