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Prevalence of Factor V Leiden (FVL, G1691A) and F-II (G20210A) Mutations in Jordanian Atherosclerotic Patients

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Abstract

Atherosclerosis is a disease of blood vessels which is caused by inherited and acquired factors and leads to different complications in the body including blood clot formation, a serious condition that can threat human life. This study aims to measure the prevalence of thrombophilia gene mutations; Factor V Leiden (FVL, G1691A) and F-II (G20210A) in Jordanian patients with atherosclerosis. Using real-time PCR, the prevalence of heterozygous and homozygous variants of FVL (G1691A) were 11.32 and 1.87% respectively. In F-II (G20210), the prevalence was 1.87 and 0.31% for heterozygous and homozygous variants, respectively. Comparing mutants’ frequencies between patients with atherosclerosis and healthy people (control) showed no significant difference suggesting no relation of these mutants and thrombus formation in atherosclerotic patients, however, among patients’ group there was a significant difference in the prevalence of FVL (G1691A) variants between male and female populations and insignificant difference for F-II (G20210). In conclusion, the results of this study showed no relation of inherited F-V and F-II gene mutations in blood clot formation in atherosclerotic patients, suggestion that blood clot formation is related only to environmental factors belong to the nature of atherosclerotic plaque which trigger platelets and clotting proteins activation.

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ACKNOWLEDGMENTS

The author is grateful to the Mutah University for providing laboratory facilities to conduct experiments and nurses at the Prince Hamza Hospital for their help in collecting the samples from patients.

Funding

This work was supported by Mutah University (grant no. 593, 2022).

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Authors and Affiliations

  1. Medical Laboratory Sciences Department at Allied Medical Sciences Faculty, Mutah University, 61710, Al-Karak, Jordan

    Ahmad Q. Jaradat

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  1. Ahmad Q. Jaradat
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Correspondence to Ahmad Q. Jaradat.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All studies were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and its later amendments. They were also approved by the Committee of Research on Human at the Jordanian Ministry of Health, protocol no. 4777 dated March 23, 2023.Each participant in the study provided a voluntary written informed consent after receiving an explanation of the potential risks and benefits, as well as the nature of the upcoming study.

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The author of this work declares that he has no conflicts of interest.

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Ahmad Q. Jaradat Prevalence of Factor V Leiden (FVL, G1691A) and F-II (G20210A) Mutations in Jordanian Atherosclerotic Patients. Biochem. Moscow Suppl. Ser. B 17, 189–193 (2023). https://doi.org/10.1134/S1990750823600358

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  • DOI: https://doi.org/10.1134/S1990750823600358

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