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Children with Hirschsprung disease exhibited alterations in host–microbial co-metabolism after pull-through operation

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Abstract

Purpose

This study aims to compare the fecal metabolome in post pull-through HD with and without HAEC patients and healthy young children using nuclear magnetic resonance (NMR) spectroscopy.

Methods

Fresh fecal samples were collected from children under 5 years of age in both post-pull-through HD patients and healthy Thai children. A total of 20 fecal samples were then analyzed using NMR spectroscopy.

Results

Thirty-four metabolites identified among HD and healthy children younger than 5 years were compared. HD samples demonstrated a significant decrease in acetoin, phenylacetylglutamine, and N-acetylornithine (corrected p value = 0.01, 0.04, and 0.004, respectively). Succinate and xylose significantly decreased in HD with HAEC group compared to HD without HAEC group (corrected p value = 0.04 and 0.02, respectively). Moreover, glutamine and glutamate metabolism, and alanine, aspartate, and glutamate metabolism were the significant pathways involved, with pathway impact 0.42 and 0.50, respectively (corrected p value = 0.02 and 0.04, respectively).

Conclusion

Differences in class, quantity, and metabolism of protein and other metabolites in young children with HD after pull-through operation were identified. Most of the associated metabolic pathways were correlated with the amino acids metabolism, which is required to maintain intestinal integrity and function.

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Data availability

Data can be accessed at Open Science Framework (https://osf.io/bvs96/).

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Acknowledgements

This work was supported by grants from Faculty of Medicine, Khon Kaen University (Grant No. IN63253) to Kanokrat Thaiwatcharamas and Thailand Science Research and Innovation (TSRI) to Watcharin Loilome.

Funding

Faculty of Medicine, Khon Kaen University, IN63253, Thailand Science Research and Innovation.

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Authors and Affiliations

  1. Department of Surgery, Division of Pediatric Surgery, Khon Kaen University, Khon Kaen, Thailand

    Kanokrat Thaiwatcharamas, Sinobol Chusilp & Patchareeporn Tanming

  2. Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

    Watcharin Loilome, Phuc N. Ho, Poramate Klanrit & Jutarop Phetcharaburanin

  3. Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand

    Watcharin Loilome, Poramate Klanrit & Jutarop Phetcharaburanin

  4. Khon Kaen University Phenome Centre, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

    Watcharin Loilome, Poramate Klanrit & Jutarop Phetcharaburanin

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Contributions

K.T., W.L., J.P., P.T., and P.K. made substantial contributions to the conception and design of the work. K.T. and J.P. made substantial contributions to the acquisition of data, analysis, and interpretation of data. J.P. and P.H. made substantial contributions to the analysis of data and the creation of new software used in the work. K.T., J.P., S.C., and W.L. drafted the work or revised it critically for important intellectual content. K.T. wrote the main manuscript text. J.P. and P.H. prepared figures 1-6. All authors reviewed the manuscript.

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Correspondence to Jutarop Phetcharaburanin.

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Thaiwatcharamas, K., Loilome, W., Ho, P.N. et al. Children with Hirschsprung disease exhibited alterations in host–microbial co-metabolism after pull-through operation. Pediatr Surg Int 40, 87 (2024). https://doi.org/10.1007/s00383-024-05667-3

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