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A magnetic nanozyme platform for bacterial colorimetric detection and chemodynamic/photothermal synergistic antibacterial therapy

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Abstract

Rapid, convenient, and sensitive detection of bacteria and development of novel antibacterial materials are conducive to accurate treatment of bacterial infection and reducing the generation of drug-resistant bacteria caused by overuse of antibiotics. A dual-function magnetic nanozyme, Fc-MBL@rGO@Fe3O4, has been constructed with broad-spectrum bacterial affinity and good peroxidase-like activity. Detection signal amplification was realized in the presence of 3,3’,5,5’-tetramethylbenzidine (TMB) with a detection limit of 26 CFU/mL. In addition, the excellent photothermal properties of Fc-MBL@rGO@Fe3O4 could realize synergistic chemodynamic/photothermal antibacterial therapy. Furthermore, the good bacterial affinity of Fc-MBL@rGO@Fe3O4 enhances the accurate and rapid attack of hydroxyl radical (·OH) on the bacterial membrane and achieves efficient sterilization (100%) at low concentration (40 µg/mL) and mild temperature (47℃). Notably, Fc-MBL@rGO@Fe3O4 has a broad spectrum of antibacterial activity against Gram-negative, Gram-positive, and drug-resistant bacteria. The magnetic nanoplatform integrating detection-sterilization not only meets the need for highly sensitive and accurate detection in different scenarios, but can realize low power density NIR-II light-responsive chemodynamic/photothermal antibacterial therapy, which has broad application prospects.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Key Research and Development Plan of China (2018YFF0212501) and National Natural Science Foundation of China (31470786).

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Authors and Affiliations

  1. Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China

    Yuyan Xue, Yanlin Wang, Hao Shen & Shaoning Yu

  2. Department of Chemistry, Fudan University, Shanghai, 200438, China

    Yuyan Xue & Qiaoyu Li

Authors
  1. Yuyan Xue
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  2. Qiaoyu Li
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  3. Yanlin Wang
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  4. Hao Shen
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  5. Shaoning Yu
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Contributions

Yuyan Xue: Conceptualization, Methodology, Data curation, Writing - original draft preparation. Qiaoyu Li: Software, Formal analysis. Yanlin Wang: Formal analysis. Hao Shen: Visualization, Reviewing, Validation, Supervision. Shaoning Yu: Editing, Supervision.

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Correspondence to Hao Shen or Shaoning Yu.

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Xue, Y., Li, Q., Wang, Y. et al. A magnetic nanozyme platform for bacterial colorimetric detection and chemodynamic/photothermal synergistic antibacterial therapy. Microchim Acta 191, 214 (2024). https://doi.org/10.1007/s00604-024-06270-y

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  • DOI: https://doi.org/10.1007/s00604-024-06270-y

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