Genomic and Metagenomic Insights into the Distribution of Nicotine-Degrading Enzymes in Human Microbiota

Title:Genomic and Metagenomic Insights into the Distribution of Nicotine-Degrading Enzymes in Human Microbiota

Volume: 25

Author(s): Ying Guan, Zhouhai Zhu, Qiyuan Peng, Meng Li, Xuan Li, Jia-Wei Yang, Yan-Hong Lu, Meng Wang and Bin-Bin Xie*

Affiliation:

• State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China

Keywords: nicotine-degrading enzymes, human microbiota, metagenomes, NicX, nicotine degradation, genome annotation

Abstract: Introduction: Nicotine degradation is a new strategy to block nicotine-induced pathology. The potential of human microbiota to degrade nicotine has not been explored.

Aims : : This study aimed to uncover the genomic potentials of human microbiota to degrade nicotine.

Method: To address this issue, we performed a systematic annotation of Nicotine-Degrading Enzymes (NDEs) from genomes and metagenomes of human microbiota. A total of 26,295 genomes and 1,596 metagenomes for human microbiota were downloaded from public databases and five types of NDEs were annotated with a custom pipeline. We found 959 NdhB, 785 NdhL, 987 NicX, three NicA1, and three NicA2 homologs.

Results: Genomic classification revealed that six phylum-level taxa, including Proteobacteria, Firmicutes, Firmicutes_A, Bacteroidota, Actinobacteriota, and Chloroflexota, can produce NDEs, with Proteobacteria encoding all five types of NDEs studied. Analysis of NicX prevalence revealed differences among body sites. NicX homologs were found in gut and oral samples with a high prevalence but not found in lung samples. NicX was found in samples from both smokers and non-smokers, though the prevalence might be different.

Conclusion: This study represents the first systematic investigation of NDEs from the human microbiota, providing new insights into the physiology and ecological functions of human microbiota and shedding new light on the development of nicotine-degrading probiotics for the treatment of smoking-related diseases.

Cite this article as:

Guan Ying, Zhu Zhouhai, Peng Qiyuan, Li Meng, Li Xuan, Yang Jia-Wei, Lu Yan-Hong, Wang Meng and Xie Bin-Bin*, Genomic and Metagenomic Insights into the Distribution of Nicotine-Degrading Enzymes in Human Microbiota, Current Genomics 2024; 25 () . https://dx.doi.org/10.2174/0113892029302230240319042208

DOI https://dx.doi.org/10.2174/0113892029302230240319042208	Print ISSN 1389-2029
Publisher Name Bentham Science Publisher	Online ISSN 1875-5488