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Variation of humic carbon and microbial communities in bauxite residue following co-application of straw and phosphogypsum

秸秆-磷石膏联用对赤泥的腐殖质组分和微生物群落的影响

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Abstract

Humification is one of the critical processes in the ecological restoration of bauxite residue deposit areas. Straw addition is widely used strategy to increase organic carbon in bauxite residue. However, the effect of straw application on the humic carbon fractions in bauxite residue is largely unknown. In this study, the accumulation of humic fractions and associated microbial communities in bauxite residue following straw application were evaluated by humus fractionation and high-throughput sequencing technology. The results showed that straw application significantly increased humic carbon fractions (humic acid and fulvic acid) and humification degree in bauxite residue. The content of humic acid and fulvic acid increased by 27.1% and 22.9% in straw-amended bauxite residue after phosphogypsum addition, respectively. The glucosidase, cellulolytic enzyme, polyphenol oxidase and peroxidase increased by 7.15–8.76 times, 5.64–7.12 times, 2.69–4.57 times and 2.59–4.24 times following the straw application. High-throughput sequencing results indicated that the operational taxonomic unit (OTU) numbers and Shannon index of both bacterial and fungal communities significantly increased following co-application of straw and phosphogypsum. In addition, co-application of straw and phosphogypsum significantly increased the relative abundance of Devosiaceae, Rhizobiaceae, Flavobacteriaceae, Caulobacteraceae, and Cellvibrionaceae in bauxite residue. These findings provide us with a biological perspective of straw on the humification process in bauxite residue.

摘要

腐殖化过程是赤泥土壤化及堆场生态修复的重要环节。本研究采用腐殖质分级和高通量测序技 术, 探究了秸秆和磷石膏联用对赤泥腐殖质组分和微生物群落的影响。结果表明, 秸秆-磷石膏联用显 著增加赤泥胡敏酸和富里酸含量, 提高微生物群落多样性和丰富度, 促进赤泥腐殖化过程。施用秸秆 后, 赤泥中葡萄糖苷酶、纤维素水解酶、多酚氧化酶和过氧化物酶分别升高7.15∼8.76 倍、5.64∼ 7.12 倍、2.69∼4.57 倍和2.59∼4.24 倍, 显著增加Devosiaceae、Rhizobiaceae、Flavobacteriaceae、 Caulobacteraceae 和Cellvibrionaceae 等具有纤维素降解和固氮功能微生物类群相对丰度, 提高微生物 群落稳定性。研究结果为外源生物质促进腐殖质累积、驱动赤泥成土进程提供科学依据。

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Authors and Affiliations

  1. School of Metallurgy and Environment, Central South University, Changsha, 410083, China

    Hao Wu  (吴昊), Xuan-zhi Zhu  (朱轩郅), Chong-jian Tang  (唐崇俭), Shi-wei Huang  (黄诗蔚), Jun Jiang  (江钧), Feng Zhu  (朱锋), Xing-wang Yang  (杨兴旺) & Sheng-guo Xue  (薛生国)

  2. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Hao Wu  (吴昊) & Wei Sun  (孙伟)

  3. Department of Life Sciences, Schrodinger Building, University of Limerick, Limerick, V94 T9PX, Ireland

    Courtney Ronan

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  1. Hao Wu  (吴昊)
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Contributions

WU Hao conducted the literature review and wrote the manuscript. ZHU Xuan-zhi determined the main physiochemical index. HUANG Shi-wei validated the proposed method with practical experiments. JIANG Jun and ZHU Feng reviewed the manuscript. YANG Xing-wang and RONAN Courtney edited the manuscript. XUE Sheng-guo, TANG Chong-jian and SUN Wei developed the overarching research goals.

Corresponding authors

Correspondence to Jun Jiang  (江钧) or Sheng-guo Xue  (薛生国).

Ethics declarations

WU Hao, ZHU Xuan-zhi, TANG Chong-jian, HUANG Shi-wei, SUN Wei, JIANG Jun, ZHU Feng, YANG Xing-wang, RONAN Courtney, XUE Sheng-guo declare that they have no conflict of interest.

Additional information

Foundation item: Project(42030711) supported by the Key Project of National Natural Science Foundation of China; Project(42177391) supported by the National Natural Science Foundation of China

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Wu, H., Zhu, Xz., Tang, Cj. et al. Variation of humic carbon and microbial communities in bauxite residue following co-application of straw and phosphogypsum. J. Cent. South Univ. 31, 460–476 (2024). https://doi.org/10.1007/s11771-024-5570-2

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