Abstract
Polyethylene (PE), a non-biodegradable plastic, is widely used in agriculture as a mulch material, which causes serious plastic pollution when it is discarded. Recent studies have described the biodeterioration of PE by bacteria, but it is difficult for a single bacterial species to effectively degrade PE plastic. We isolated two strains with PE-degrading ability, Bacillus cereus (E1) and Rhodococcus equi (E3), from the soil attached to plastic waste on the south side of Mount Tai, China, using a medium with PE plastic as the only carbon source. By clear zone area analysis, we found that E1 mixed with E3 could improve the degradation of PE plastics. The mixture of E1 and E3 was incubated for 110 days in a medium containing PE and mulch film as the only carbon source, respectively. After 110 days, a decrease in pH and mass was observed. Obvious slits and depressions were observed on the surface of the PE film and the mulch films using scanning electron microscopy. The surface hydrophobicity of both films decreased, and FTIR revealed the formation of new oxidation groups on their surfaces during the degradation process and the destruction of the original CH2 long chains of PE. Besides, we found that surface of the mulch films contained more viable bacteria than the liquid medium. In conclusion, we identified two PE-degrading strains whose mixture can effectively degrade mulch film than pure PE film. Our results provide a reference for understanding PE plastic degradation pathways and their associated degradation processes.
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All data generated or analyzed during the present study are available from the corresponding author upon request.
References
Abd El-Rehim HA, Hegazy E-SA, Ali AM, Rabie AM (2004) Synergistic effect of combining UV-sunlight–soil burial treatment on the biodegradation rate of LDPE/starch blends. J Photochem Photobiol A: Chem 163(3):547–556. https://doi.org/10.1016/j.jphotochem.2004.02.003
Adame-Gomez R, Cruz-Facundo IM, Garcia-Diaz LL, Ramirez-Sandoval Y, Perez-Valdespino A, Ortuno-Pineda C, Santiago-Dionisio MC, Ramirez-Peralta A (2020) Biofilm production by enterotoxigenic strains of bacillus cereus in different materials and under different environmental conditions. Microorganisms 8(7):1071. https://doi.org/10.3390/microorganisms8071071
Amobonye A, Bhagwat P, Singh S, Pillai S (2021) Plastic biodegradation: frontline microbes and their enzymes. Sci Total Environ 759:143536. https://doi.org/10.1016/j.scitotenv.2020.143536
Auta HS, Emenike CU, Fauziah SH (2017) Screening of strains isolated from mangrove ecosystems in Peninsular Malaysia for microplastic degradation. Environ Pollut 231(Pt 2):1552–1559. https://doi.org/10.1016/j.envpol.2017.09.043
Bardaji DKR, Moretto JAS, Furlan JPR, Stehling EG (2020) A mini-review: current advances in polyethylene biodegradation. World J Microbiol Biotechnol 36(2):32. https://doi.org/10.1007/s11274-020-2808-5
Brodhagen M, Goldberger JR, Hayes DG, Inglis DA, Marsh TL, Miles C (2017) Policy considerations for limiting unintended residual plastic in agricultural soils. Environ Sci Policy 69:81–84. https://doi.org/10.1016/j.envsci.2016.12.014
Cai L, Wang J, Peng J, Wu Z, Tan X (2018) Observation of the degradation of three types of plastic pellets exposed to UV irradiation in three different environments. Sci Total Environ 628–629:740–747. https://doi.org/10.1016/j.scitotenv.2018.02.079
Cai Z, Li M, Zhu Z, Wang X, Huang Y, Li T, Gong H, Yan M (2023) Biological degradation of plastics and microplastics: a recent perspective on associated mechanisms and influencing factors. Microorganisms 11(7):1661. https://doi.org/10.3390/microorganisms11071661
Chaudhary AK, Chaitanya K, Dalmia R, Vijayakumar RP (2021) Synergistic effect of UV, thermal, and chemical treatment on biological degradation of low-density polyethylene (LDPE) by Thermomyces lanuginosus. Environ Monit Assess 193(8):513. https://doi.org/10.1007/s10661-021-09296-4
Chen Z, Zhao W, Xing R, Xie S, Yang X, Cui P, Lu J, Liao H, Yu Z, Wang S, Zhou S (2020) Enhanced in situ biodegradation of microplastics in sewage sludge using hyperthermophilic composting technology. J Hazard Mater 384:121271. https://doi.org/10.1016/j.jhazmat.2019.121271
Das MP, Kumar S (2015) An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech 5(1):81–86. https://doi.org/10.1007/s13205-014-0205-1
Du Y, Liu X, Dong X, Yin Z (2022) A review on marine plastisphere: biodiversity, formation, and role in degradation. Comput Struct Biotechnol J 20:975–988. https://doi.org/10.1016/j.csbj.2022.02.008
Gao R, Sun C (2021) A marine bacterial community capable of degrading poly(ethylene terephthalate) and polyethylene. J Hazard Mater 416:125928. https://doi.org/10.1016/j.jhazmat.2021.125928
Gong M, Yang G, Zhuang L, Zeng EY (2019) Microbial biofilm formation and community structure on low-density polyethylene microparticles in lake water microcosms. Environ Pollut 252(Pt A):94–102. https://doi.org/10.1016/j.envpol.2019.05.090
Gradus RHJM, Nillesen PHL, Dijkgraaf E, van Koppen RJ (2017) A cost-effectiveness analysis for incineration or recycling of dutch household plastic waste. Ecol Econ 135:22–28. https://doi.org/10.1016/j.ecolecon.2016.12.021
Hahladakis JN, Velis CA, Weber R, Iacovidou E, Purnell P (2018) An overview of chemical additives present in plastics: migration, release, fate and environmental impact during their use, disposal and recycling. J Hazard Mater 344:179–199. https://doi.org/10.1016/j.jhazmat.2017.10.014
Han YN, Wei M, Han F, Fang C, Wang D, Zhong YJ, Guo CL, Shi XY, Xie ZK, Li FM (2020) Greater biofilm formation and increased biodegradation of polyethylene film by a microbial consortium of Arthrobacter sp. and Streptomyces sp. Microorganisms 8(12):1979. https://doi.org/10.3390/microorganisms8121979
Harshvardhan K, Jha B (2013) Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India. Mar Pollut Bull 77(1–2):100–106. https://doi.org/10.1016/j.marpolbul.2013.10.025
Hou L, Xi J, Chen X, Li X, Ma W, Lu J, Xu J, Lin YB (2019) Biodegradability and ecological impacts of polyethylene-based mulching film at agricultural environment. J Hazard Mater 378:120774. https://doi.org/10.1016/j.jhazmat.2019.120774
Hou L, Xi J, Liu J, Wang P, Xu T, Liu T, Qu W, Lin YB (2022) Biodegradability of polyethylene mulching film by two Pseudomonas bacteria and their potential degradation mechanism. Chemosphere 286(Pt 3):131758. https://doi.org/10.1016/j.chemosphere.2021.131758
Huang D, Xu Y, Lei F, Yu X, Ouyang Z, Chen Y, Jia H, Guo X (2021) Degradation of polyethylene plastic in soil and effects on microbial community composition. J Hazard Mater 416:126173. https://doi.org/10.1016/j.jhazmat.2021.126173
Inderthal H, Tai SL, Harrison STL (2021) Non-hydrolyzable plastics - an interdisciplinary look at plastic bio-oxidation. Trends Biotechnol 39(1):12–23. https://doi.org/10.1016/j.tibtech.2020.05.004
Ko SH, Lebeault JM (1999) Effect of a mixed culture on co-oxidation during the degradation of saturated hydrocarbon mixture. J Appl Microbiol 87(1):72–79. https://doi.org/10.1046/j.1365-2672.1999.00797.x
Kumar MV, Sheela AM (2021) Effect of plastic film mulching on the distribution of plastic residues in agricultural fields. Chemosphere 273:128590. https://doi.org/10.1016/j.chemosphere.2020.128590
Kumar SJ, Teotia UVS, Singh Y (2017) A comprehensive review on microbial degradation of plastic waste. J Appl Pharm Res 5(4):08–12. https://doi.org/10.18231/2348-0335.2017.0009
Lear G, Kingsbury JM, Franchini S, Gambarini V, Maday SDM, Wallbank JA, Weaver L, Pantos O (2021) Plastics and the microbiome: impacts and solutions. Environ Microbiome 16(1):2. https://doi.org/10.1186/s40793-020-00371-w
Liu S, Wang J, Zhu J, Wang J, Wang H, Zhan X (2021) The joint toxicity of polyethylene microplastic and phenanthrene to wheat seedlings. Chemosphere 282:130967. https://doi.org/10.1016/j.chemosphere.2021.130967
Mohandass R, Rout P, Jiwal S, Sasikala C (2012) Biodegradation of benzo[a]pyrene by the mixed culture of and isolated from the petrochemical industry. J Environ Biol 33(6):985–989
Montazer Z, Habibi Najafi MB, Levin DB (2021) In vitro degradation of low-density polyethylene by new bacteria from larvae of the greater wax moth, Galleria mellonella. Can J Microbiol 67(3):249–258. https://doi.org/10.1139/cjm-2020-0208
Muhonja CN, Makonde H, Magoma G, Imbuga M (2018) Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi-Kenya. PLoS One 13(7):e0198446. https://doi.org/10.1371/journal.pone.0198446
Nyamjav I, Jang Y, Park N, Lee YE, Lee SKY (2023) Physicochemical and structural evidence that isolated from the gut of waxworms (larvae) biodegrades polypropylene efficiently in vitro. J Polym Environ 31(10):4274–4287. https://doi.org/10.1007/s10924-023-02878-y
Park SY, Kim CG (2019) Biodegradation of micro-polyethylene particles by bacterial colonization of a mixed microbial consortium isolated from a landfill site. Chemosphere 222:527–533. https://doi.org/10.1016/j.chemosphere.2019.01.159
Peng BY, Chen Z, Chen J, Yu H, Zhou X, Criddle CS, Wu WM, Zhang Y (2020) Biodegradation of polyvinyl chloride (PVC) in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. Environ Int 145:106106. https://doi.org/10.1016/j.envint.2020.106106
Qi R, Jones DL, Li Z, Liu Q, Yan C (2020) Behavior of microplastics and plastic film residues in the soil environment: a critical review. Sci Total Environ 703:134722. https://doi.org/10.1016/j.scitotenv.2019.134722
Rillig MC, Ziersch L, Hempel S (2017) Microplastic transport in soil by earthworms. Sci Rep 7(1):1362. https://doi.org/10.1038/s41598-017-01594-7
Sarker RK, Chakraborty P, Sarkar S, Ghosh MM, Tribedi P (2021) Bioaugmentation of Enterobacter cloacae AKS7 causes an enhanced degradation of low-density polyethylene (LDPE) in soil: a promising approach for the sustainable management of LDPE waste. Arch Microbiol 204(1):74. https://doi.org/10.1007/s00203-021-02645-4
Singh S, Singh BB, Chandra R (2009) Biodegradation of phenol in batch culture by pure and mixed strains of sp and. Pol J Microbiol 58(4):319–325
Singh Jadaun J, Bansal S, Sonthalia A, Rai AK, Singh SP (2022) Biodegradation of plastics for sustainable environment. Bioresour Technol 347:126697. https://doi.org/10.1016/j.biortech.2022.126697
Sivan A, Szanto M, Pavlov V (2006) Biofilm development of the polyethylene-degrading bacterium Rhodococcus ruber. Appl Microbiol Biotechnol 72(2):346–352. https://doi.org/10.1007/s00253-005-0259-4
Soleimani Z, Gharavi S, Soudi M, Moosavi-Nejad Z (2021) A survey of intact low-density polyethylene film biodegradation by terrestrial Actinobacterial species. Int Microbiol 24(1):65–73. https://doi.org/10.1007/s10123-020-00142-0
Spina F, Tummino ML, Poli A, Prigione V, Ilieva V, Cocconcelli P, Puglisi E, Bracco P, Zanetti M, Varese GC (2021) Low density polyethylene degradation by filamentous fungi. Environ Pollut 274:116548. https://doi.org/10.1016/j.envpol.2021.116548
Sudhakar M, Doble M, Murthy PS, Venkatesan R (2008) Marine microbe-mediated biodegradation of low- and high-density polyethylenes. Int Biodeterior Biodegradation 61(3):203–213. https://doi.org/10.1016/j.ibiod.2007.07.011
Suyamud B, Thiravetyan P, Gadd GM, Panyapinyopol B, Inthorn D (2019) Bisphenol A removal from a plastic industry wastewater by Dracaena sanderiana endophytic bacteria and Bacillus cereus NI. Int J Phytorem 22(2):167–175. https://doi.org/10.1080/15226514.2019.1652563
Tribedi P, Gupta AD, Sil AK (2015) Adaptation of Pseudomonas sp. AKS2 in biofilm on low-density polyethylene surface: an effective strategy for efficient survival and polymer degradation. Bioresources Bioprocess 2:1–10. https://doi.org/10.1186/s40643-015-0044-x
Vazquez-Boland JA, Meijer WG (2019) The pathogenic actinobacterium Rhodococcus equi: what’s in a name? Mol Microbiol 112(1):1–15. https://doi.org/10.1111/mmi.14267
Wang J, Liu X, Dai Y, Ren J, Li Y, Wang X, Zhang P, Peng C (2020) Effects of co-loading of polyethylene microplastics and ciprofloxacin on the antibiotic degradation efficiency and microbial community structure in soil. Sci Total Environ 741:140463. https://doi.org/10.1016/j.scitotenv.2020.140463
Wang T, Ma Y, Ji R (2021) Aging processes of polyethylene mulch films and preparation of microplastics with environmental characteristics. Bull Environ Contam Toxicol 107(4):736–740. https://doi.org/10.1007/s00128-020-02975-x
Yang J, Yang Y, Wu WM, Zhao J, Jiang L (2014) Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms. Environ Sci Technol 48(23):13776–13784. https://doi.org/10.1021/es504038a
Yang Y, Wang J, Xia M (2020) Biodegradation and mineralization of polystyrene by plastic-eating superworms Zophobas atratus. Sci Total Environ 708:135233. https://doi.org/10.1016/j.scitotenv.2019.135233
Yang X, Li L, Zhao W, Li X, Mu Y, Chen M, Wu X (2022) Substitute for polyethylene (PE) films: a novel cow dung-based liquid mulch on silage cornfields. PLoS One 17(7):e0271273. https://doi.org/10.1371/journal.pone.0271273
Zampolli J, Orro A, Vezzini D, Di Gennaro P (2022) Genome-based exploration of species for plastic-degrading genetic determinants using bioinformatic analysis. Microorganisms 10(9):1846. https://doi.org/10.3390/microorganisms10091846
Zhang M, Zhao Y, Qin X, Jia W, Chai L, Huang M, Huang Y (2019) Microplastics from mulching film is a distinct habitat for bacteria in farmland soil. Sci Total Environ 688:470–478. https://doi.org/10.1016/j.scitotenv.2019.06.108
Zhang J, Gao D, Li Q, Zhao Y, Li L, Lin H, Bi Q, Zhao Y (2020) Biodegradation of polyethylene microplastic particles by the fungus Aspergillus flavus from the guts of wax moth Galleria mellonella. Sci Total Environ 704:135931. https://doi.org/10.1016/j.scitotenv.2019.135931
Acknowledgements
The authors wish to thank Claudine Elmerich for helpful and insightful comments on the manuscript.
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This work was funded by the National Natural Science Foundation of China (32270065) and the Shandong Key Research and Development Program (2021CXGC010804).
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X.D.: Methodology, Analysis, Writing-Original Draft.
D.W.: Formal analysis, Analysis, Review &; Editing.
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Liu, X., Dong, X., Wang, D. et al. Biodeterioration of polyethylene by Bacillus cereus and Rhodococcus equi isolated from soil. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00509-7
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DOI: https://doi.org/10.1007/s10123-024-00509-7