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How Does Curtobacterium Produce a Bright Flash-Yellow Color?

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Abstract

Curtobacterium, a bright flash-yellow color-producing bacterium, has been isolated from the body fluid of the pupae of Graphium sarpedon. Several experiments were conducted to identify three mechanisms by which this bacterium expresses a vibrant color. The first is caused by yellow pigmentation; the second is due to a substance that absorbs blue light and emits green fluorescence; and the third is a process that excludes brownish colors by inhibiting melanin synthesis. In particular, we focused on melanin synthesis inhibition and used the water-soluble fraction of Curtobacterium and the melanin synthase in vitro human tyrosinase activity assay to examine its inhibitory effects on monophenolase activity, which converts Tyr to L-3,4-dihydroxyphenylalanine (L-DOPA), and diphenolase activity, which uses L-DOPA as a substrate to produce L-dopaquinone. The results demonstrated that the water-soluble fraction of bacterial secretion inhibited monophenolase activity but not diphenolase activity. Additionally, we discovered a compound with the molecular weight of approximately 379.2 Da and the tyrosinase inhibitory effect in which two protons were bound in the absence of copper ions and copper ions were bound instead when they were present in the water-soluble fraction of the bacterial secretion. Since the enzymatic activity of tyrosinase is copper ion-dependent, Curtobacterium may be able to suppress the browning caused by the copper-binding compound-induced oxidation of phenols, such as Tyr, allowing it to express a bright flash-yellow color.

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ACKNOWLEDGMENTS

We would like to acknowledge Editage (https://www.editage.com/) for English language editing.

Funding

This project was supported by the Nakatani Foundation of Japan (Grant no.: 23P08).

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Authors and Affiliations

  1. Department of Chemistry and Biology, National Institute of Technology, Fukui College, 916-8507, Geshi-cho, Sabae City, Fukui, Japan

    T. Kawamura, S. Takanawa, H. Ashida, S. Muranaka, A. Murota, S. Kota, A. Maeda, R. Hashimoto, E. Matsui & K. Takayama

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  1. T. Kawamura
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  2. S. Takanawa
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  3. H. Ashida
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  4. S. Muranaka
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Correspondence to T. Kawamura.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

As this study solely focused on bacterial research and did not involve human and animal subjects, ethics approval and consent to participate were not required. The bacteria used in the research are non-pathogenic and comply with the regulations of the Ministry of Education, Culture, Sports, Science and Technology.

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Kawamura, T., Takanawa, S., Ashida, H. et al. How Does Curtobacterium Produce a Bright Flash-Yellow Color?. Appl Biochem Microbiol (2024). https://doi.org/10.1134/S0003683823602895

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