Abstract
Curtobacterium sp. strain WW7 is a Gram-positive, non-motile, orange rod-shaped bacterium isolated from branches of wild willow (Salix sitchensis) trees. The WW7T strain has optimum growth in the temperature range between 25 and 30 °C, a pH range of 6–7.7, and tolerates up to 5.5% (w/v) of NaCl. The genome sequencing of strain WW7T revealed a genome size of approximately 3.8 Mbp and a G + C content of 71.3 mol%. The phylogenomic analyses support the WW7T affiliation to a novel Curtobacterium lineage, with Curtobacterium herbarum being the closest type-strain. Chemotaxonomic analysis indicates that the carbon sources assimilation profile of strain WW7T was similar to the type strains, i.e. Curtobacterium luteum, Curtobacterium albidum, and Curtobacterium flaccumfaciens, while no assimilation of the organic acids succinate, alpha-ketobutyrate, mono methyl-succinate, and lactate was observed. Finally, fatty acid methyl ester analysis identifies anteiso-C15:0 and anteiso-C17:0 as major cellular fatty acids which is a common feature for members of the Curtobacterium genus. Based on the results of phylogenomic and chemotaxonomic analyses, strain WW7T represents a novel Curtobacterium lineage, for which the name Curtobacterium salicis sp. nov. is proposed. The type strain is WW7T (DSM 34805T-NRRL B-68078T).
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Data availability
Strain WW7T genome sequence and annotation have been submitted to the NCBI Assembly database under the accession number GCF_011759505.1. The partial 16S rRNA gene sequence of the WW7T strain has been deposited to the NCBI Nucleotide database with the accession number KU523564.1. Strain WW7T culture are available in the Agricultural Research Culture Collection (NRRL) and German Collection of Microorganisms and Cell Cultures (DSMZ) under the accession codes NRRL B-68078T and DSM 34805T, respectively.
Code availability
Agricultural Research Culture Collection (NRRL) code NRRL B-68078. German Collection of Microorganisms and Cell Cultures (DSMZ) code DSM 34805.
Abbreviations
- dDDH:
-
Digital DNA–DNA hybridization
- FA:
-
Fatty acid
- FAME:
-
Fatty acid methyl ester analysis
- GTDB-tk:
-
Genome taxonomy database
- MS:
-
Murashige and Skoog
- TYGS:
-
Type-strain genome server
- PM:
-
Phenotype microarray
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JF conceived the experiments with help from AF. AF conducted the genomic and bioinformatics analyses. JF and DB conducted chemotaxonomic analyses. SLD isolated the strain. All authors contributed to writing the manuscript.
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Intrinsyx Bio is developing agricultural products based on the microbial strain in this study.
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Freeman, J., Firrincieli, A., Baker, D. et al. Curtobacterium salicis sp. nov., isolated from willow tree stems in Washington state. Antonie van Leeuwenhoek 117, 62 (2024). https://doi.org/10.1007/s10482-024-01956-y
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DOI: https://doi.org/10.1007/s10482-024-01956-y