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Polyhydroxyalkanoate Production Using Sulfuric Acid Pre-Treated Wheat Straw (SAPWS) as a Feedstock: RSM (Response Surface Methodology)-Based Optimization Using Bacillus paranthracis RSKS-3

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Abstract

The increasing number of plastic debris produced worldwide and its detrimental impacts on the environment and human health have led to rising demand for bio-plastic, or polyhydroxyalkanoate, as a replacement to synthetic plastic manufactured from petroleum. The usefulness of agricultural waste as a prime source of carbon for Polyhydroxyalkanoate production and aids in the valorization of the waste. The study optimizes sulfuric acid pre-treated wheat straw (SAPWS) along with inoculum size, temperature, and incubation period using Box-Behnken design (BBD). The maximum Polyhydroxyalkanoate (PHA) yield (0.250 g/L) and productivity (0.521%) by Bacillus paranthracis RSKS-3. were found at 10 g/L SAPWS, 5% inoculum size, 7.4 pH, and 48 h of the incubation period. The observed estimations were in statistical accord with the anticipated values of 0.247 g/L and 0.508%. Based on PHA production using SAPWS as a carbon source, 1.2 g/L was produced with was 5.29-fold increment as compared to standard glucose (10 g/L) that produced 0.34 g/L PHA. The characteristic properties of extracted PHA were similar to the standard PHA. Conclusively, agricultural waste possibly will be utilized as an alternative and a renewable feedstock for green synthesis of PHA production.

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Authors and Affiliations

  1. Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India

    Rohan Samir Kumar Sachan, Inderpal Devgon, Khushboo Rajput, Mukesh Kumar, Aanchal Sharma & Arun Karnwal

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  1. Rohan Samir Kumar Sachan
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  2. Inderpal Devgon
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  3. Khushboo Rajput
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  4. Mukesh Kumar
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  5. Aanchal Sharma
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Correspondence to Arun Karnwal.

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Sachan, R.S.K., Devgon, I., Rajput, K. et al. Polyhydroxyalkanoate Production Using Sulfuric Acid Pre-Treated Wheat Straw (SAPWS) as a Feedstock: RSM (Response Surface Methodology)-Based Optimization Using Bacillus paranthracis RSKS-3. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-024-01261-2

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