Abstract
Optimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydrolysis temperature, and 94-min hydrolysis time. The resulting SBGH obtained at 19.92% DH exhibited the DPPH (24.28 µM TE/mg protein), ABTS (34.47 µM TE/mg protein), TAO (12.01 µg AAE/mg protein), and ACE inhibitory (4.91 µg/mg protein) activity. Furthermore, SBGH at 100 µg/ml displayed osteogenic property without any toxic effects on MC3T3-E1 cells. Besides, the protein content of rohu swim bladder gelatin (SBG) and SBGH was 93.68% and 94.98%, respectively. Both SBG and SBGH were rich in glycine, proline, glutamic acid, alanine, arginine, and hydroxyproline amino acids. Therefore, SBGH could be an effective nutraceutical in functional food development.
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Data Availability
The datasets generated and/or analyzed during the current study are accessible upon reasonable request from the corresponding author.
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Acknowledgements
We thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the award of the Research Fellowship. The authors would also like to express their sincere thanks to the Director, CSIR-Central Food Technological Research Institute, Mysuru, India, for granting permission to publish this work.
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The authors acknowledge the financial assistance received from DST-SERB (ECR/2015/000215), India.
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BWK, methodology, investigation, analysis, and writing. KP, methodology and analysis. SSK, methodology, analysis, and writing. DR, methodology and analysis. KS, supervision, analysis, and interpretation. TGK, conception, design, funding acquisition, analysis and interpretation, and drafting the manuscript.
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Kanwate, B.W., Patel, K., Karkal, S.S. et al. Production of Antioxidant, Angiotensin-Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder. Mar Biotechnol 26, 404–420 (2024). https://doi.org/10.1007/s10126-024-10305-z
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DOI: https://doi.org/10.1007/s10126-024-10305-z