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Effect of plasma activated water Immersion on Broccoli Seed Germination and Nutritional Quality of Sprouts

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Abstract

In the present study, plasma activated water (PAW) immersion with different discharge times were applied to investigate their effects on broccoli seed germination, sprouts growth, antioxidant enzyme activities, polyphenol and anthocyanin content as well as sulforaphane formation. The seed germination rate, germination index, vigor index, water absorption, wettability and sprouts growth were promoted by PAW treatments, and plasma discharge for 30 min (PAW30) treatment showed the best germination rate and sprouts growth. No significant difference was found in color, polyphenol and anthocyanin content as well as myrosinase activity among different PAW treatments. The superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione peroxidase (GPX) enzyme activities of broccoli sprouts were enhanced significantly by all PAW treatments. Besides, PAW15, PAW30 and PAW60 treatments also increased significantly the sulforaphane formation of 5 d sprouts, which increased by 7.09%, 10.34% and 5.43% compared to the control, respectively. Therefore, these data indicated that seeds immersed with PAW30 could be used as a promising strategy to enhance the seed germination and quality of broccoli sprouts.

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References

  • Ali M, Cheng JH, Sun D-W (2021) Effects of dielectric barrier discharge cold plasma treatments on degradation of anilazine fungicide and quality of tomato (Lycopersicon esculentum Mill) juice. Int J Food Sci Technol 56:69–75

    Article  CAS  Google Scholar 

  • Bormashenko E, Grynyov R, Bormashenko Y, Drori E (2012) Cold radiofrequency plasma treatment modifies wettability and germination speed of plant seeds. Sci Rep 2:741

    Article  PubMed  PubMed Central  Google Scholar 

  • Bousquet J, Le MV, Blain H, Czarlewski W, Zuberbier T, La TR, Pizarro LN, Reynes J, Bedbrook A, Cristol JP, Cruz AA, Fiocchi A, Haahtela T, Iaccarino G, Klimek L, Kuna P, Melen E, Mullol J, Samolinski B, Valiulis A, Anto JM (2021) Efficacy of broccoli and glucoraphanin in COVID-19: from hypothesis to proof-of-concept with three experimental clinical cases. World Allergy Organ J 14:100498

    Article  CAS  PubMed  Google Scholar 

  • Chiara LP, Dana Z, Agata L, Daniela B, Fabio P, Pietro F, Brijesh T, Paula B, Cullen PJ (2018) Plasma activated water and airborne ultrasound treatments for enhanced germination and growth of soybean. Innov Food Sci Emerg Technol 49:13–19

    Article  Google Scholar 

  • Esua OJ, Sun D-W, Cheng JH, Li JL (2022) Evaluation of storage quality of vacuum-packaged silver pomfret (Pampus argenteus) treated with combined ultrasound and plasma functionalized liquids hurdle technology. Food Chem 391:133237

    Article  CAS  PubMed  Google Scholar 

  • Filatova I, Azharonok V, Kadyrov M, Beljavsky V, Antonuk A (2011) The effect of plasma treatment of seeds of some grain and legumes on their sowing quality and productivity. Rom J Phys 56:139–143

    Google Scholar 

  • Gao SW, Zhu YM, Wang QL, Cao Q, Wang CJ (2022) Effects of cold plasma treatment on production and contents of bioactive substances in broccoli sprouts. J Food Sci Technol 43:117–123 ((in Chinese with English abstract))

    Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch 48:909–930

    Article  CAS  Google Scholar 

  • Gomes BR, Siqueira-Soares RdC, Santos WDD, Marchiosi R, Soares AR, Ferrarese-Filho O (2014) The effects of dopamine on antioxidant enzymes activities and reactive oxygen species levels in soybean roots. Plant Signal Behav 9:977704

    Article  Google Scholar 

  • Guo LP, Yang RQ, Wang ZY, Guo QH, Gu ZX (2014) Effect of NaCl stress on health-promoting compounds and antioxidant activity in the sprouts of three broccoli cultivars. Int J Food Sci Nutri 65:476–481

    Article  CAS  Google Scholar 

  • Guo LP, Yang RQ, Zhou YL, Gu ZX (2016) Heat and hypoxia stresses enhance the accumulation of aliphatic glucosinolates and sulforaphane in broccoli sprouts. Eur Food Res Technol 242:107–116

    Article  CAS  Google Scholar 

  • Guo LP, Zhu YL, Wang FW (2018) Calcium sulfate treatment enhances bioactive compounds and antioxidant capacity in broccoli sprouts during growth and storage. Postharvest Biol Tec 139:12–19

    Article  CAS  Google Scholar 

  • Ishibashi Y, Koda Y, Zheng SH, Yuasa T, Iwaya-Inoue M (2013) Regulation of soybean seed germination through ethylene production in response to reactive oxygen species. Ann Bot 111:95–102

    Article  CAS  PubMed  Google Scholar 

  • Jiang AL, Zuo JH, Zheng QL, Guo L, Gao LP, Zhao SG, Wang Q, Hu WZ (2019) Red LED irradiation maintains the postharvest quality of broccoli by elevating antioxidant enzyme activity and reducing the expression of senescence-related genes. Sci Hortic 251:73–79

    Article  CAS  Google Scholar 

  • Judee F, Simon S, Bailly C, Dufour T (2018) Plasma-activation of tap water using DBD for agronomy applications: Identification and quantification of long lifetime chemical species and production/consumption mechanisms. Water Res 133:47–59

    Article  CAS  PubMed  Google Scholar 

  • Liu C, Mao B, Yuan D, Chu C, Duan M (2021) Salt tolerance in rice: physiological responses and molecular mechanisms. Crop J 10:13–25

    Article  Google Scholar 

  • López-Chillón MT, Carazo-Díaz C, Prieto-Merino D, Zafrilla P, Moreno DA, Villaño D (2019) Effects of long-term consumption of broccoli sprouts on inflammatory markers in overweight subjects. Clin Nutr 38:745–752

    Article  PubMed  Google Scholar 

  • Lukes P, Dolezalova E, Sisrova I, Clupek M (2014) Aqueous-phase chemistry and bactericidal effects from an air discharge plasma in contact with water: evidence for the formation of peroxynitrite through a pseudo-second-order post-discharge reaction of H2O2and HNO2. Plasma Sources Sci T 23:015019

    Article  CAS  Google Scholar 

  • Lyublinskaya OG, Pugovkina NA, Borisov YG, Zenin VV, Nikolsky NN (2015) How to assess reactive oxygen species (ROS) concentration instead of ROS level in the cell and why the quantitative redox biology approach is useful for the analysis of ROS homeostasis in embryonic stem cells: overcoming misconceptions. Free Rad Biol Med 87:S16

    Article  Google Scholar 

  • Ma RN, Wang GM, Tian Y, Wang KL, Zhang J, Fang J (2015) Non-thermal plasma-activated water inactivation of food-borne pathogen on fresh produce. J Hazard Mater 300:643–651

    Article  CAS  PubMed  Google Scholar 

  • Martinez-Villaluenga C, Penas E, Ciska E, Piskula MK, Kozlowska H, Vidal-Valverde C, Frias J (2010) Time dependence of bioactive compounds and antioxidant capacity during germination of different cultivars of broccoli and radish seeds. Food Chem 120:710–716

    Article  CAS  Google Scholar 

  • Medina S, Domínguez-Perles R, Moreno DA, García-Viguera C, Ferreres F, Gil JI, Gil-Izquierdo Á (2015) The intake of broccoli sprouts modulates the inflammatory and vascular prostanoids but not the oxidative stress-related isoprostanes in healthy humans. Food Chem 173:1187–1194

    Article  CAS  PubMed  Google Scholar 

  • Ndiffo Y, Švubová R, Kostolani D, Kyzek S, Machala Z (2020) The effect of water activated by nonthermal air plasma on the growth of farm plants: case of maize and barley. Plasma Processes Polym 18:1–16

    Google Scholar 

  • Ožbolt L, Kreft S, Kreft I, Germ M, Stibilj V (2008) Distribution of selenium and phenolics in buckwheat plants grown from seeds soaked in Se solution and under different levels of UV-B radiation. Food Chem 110:691–696

    Article  Google Scholar 

  • Pan JJ, Wang HP, You QG, Cao R, Sun GL, Yu DQ (2023) Jasmonate-regulated seed germination and crosstalk with other phytohormones. J Exp Bot 74:1162–1175

    Article  CAS  PubMed  Google Scholar 

  • Pan Y, Zhang Y, Cheng JH, Sun D-W (2020) Inactivation of listeria monocytogenes at various growth temperatures by ultrasound pretreatment and cold plasma. LWT-Food Sci Technol 118:108635

    Article  CAS  Google Scholar 

  • Patel B, Mann GE, Chapple SJ (2018) Concerted redox modulation by sulforaphane alleviates diabetes and cardiometabolic syndrome. Free Radic Biol Med 122:150–160

    Article  CAS  PubMed  Google Scholar 

  • Pérez-Balibrea S, Moreno DA, García-Viguera C (2011) Genotypic effects on the phytochemical quality of seeds and sprouts from commercial broccoli cultivars. Food Chem 125:348–354

    Article  Google Scholar 

  • Perinban S, Orsat V, Gariepy Y, Lyew D, Raghavan V (2022) Evaluation of plasma-activated water characteristics and its process optimization. J Food Prot 45:100029

    Google Scholar 

  • Porto CL, Ziuzina D, Los A, Boehm D, Palumbo F, Favia P, Tiwari B, Bourke P, Cullen PJ (2018) Plasma activated water and airborne ultrasound treatments for enhanced germination and growth of soybean. Innov Food Sci Emerg 49:13–19

    Article  Google Scholar 

  • Priatama RA, Pervitasari AN, Park S, Park SJ, Lee YK (2022) Current Advancements in the Molecular Mechanism of Plasma Treatment for Seed Germination and Plant Growth. Int J Mol Sci 23:4609

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qiu ZB, Li JT, Zhang MM, Bi ZZ, Li ZL (2013) He–Ne laser pretreatment protects wheat seedlings against cadmium-induced oxidative stress. Ecotoxicol Environ Saf 88:135–141

    Article  CAS  PubMed  Google Scholar 

  • Ramazzina I, Lolli V, Lacey K, Tappi S, Rocculi P, Rinaldi M (2022) Fresh-cut eruca sativa treated with plasma activated water (PAW): evaluation of antioxidant capacity, polyphenolic profile and redox status in Caco2 Cells. Nutrients 14:5337

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ranieri P, Sponsel N, Kizer J, Rojas-Pierce M, Hernández R, Gatiboni L, Grunden A, Stapelmann K (2020) Plasma agriculture: review from the perspective of the plant and its ecosystem. Plasma Processes Polym 18:1–24

    Google Scholar 

  • Sajib SA, Billah M, Mahmud S, Miah M, Hossain F, Omar FB, Roy NC, Hoque KMF, Talukder MR, Kabir AH (2020) Plasma activated water: the next generation eco-friendly stimulant for enhancing plant seed germination, vigor and increased enzyme activity, a study on black gram (Vigna mungo L). Plasma Chem and Plasma P 40:119–143

    Article  CAS  Google Scholar 

  • Selcuk M, Oksuz L, Basaran P (2008) Decontamination of grains and legumes infected with Aspergillus spp. and Penicillum spp. by cold plasma treatment. Bioresour 99:5104–5109

    Article  CAS  Google Scholar 

  • Sera B, Spatenka P, Sery M, Vrchotova N, Hruskova I (2010) Influence of plasma treatment on wheat and oat germination and early growth. Plasma Sci 38:2963–2968

    Article  Google Scholar 

  • Será B, Stranák V, Serý M, Tichý M, Spatenka P (2008) Germination of chenopodium album in response to microwave plasma treatment. Plasma Sources Sci Tech 10:616

    Google Scholar 

  • Shen J, Tian Y, Li YL, Ma RN, Zhang Q, Zhang J, Fang J (2016) Bactericidal effects against S. aureus and physicochemical properties of plasma activated water stored at different temperatures. Sci Rep 6:28505

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sipos L, Nyitrai Á, Szabó D, Urbin Á, Nagy BV (2021) Former and potential developments in sensory color masking – Review. Trends Food Sci Technol 111:1–11

    Article  CAS  Google Scholar 

  • Sivachandiran L, Khacef A (2017) Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: combined effect of seed and water treatment. RSC Adv 7:1822

    Article  CAS  Google Scholar 

  • Su NN, Liu Z, Wang L, Li YY, Niu MY, Chen X, Cui J (2022) Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO. BMC Plant Biol 22:224

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thirumdas R, Kothakota A, Annapure U, Siliveru K, Blundell R, Gatt R, Valdramidis VP (2018) Plasma activated water (PAW): Chemistry, physico-chemical properties, applications in food and agriculture. Trends Food Sci Tech 77:21–31

    Article  CAS  Google Scholar 

  • Wang N, Hatcher DW, Gawalko EJ (2008) Effect of variety and processing on nutrients and certain anti-nutrients in field peas (Pisum sativum). Food Chem 111:132–138

    Article  CAS  Google Scholar 

  • Wild S, Kesmodel LL (2001) High resolution electron energy loss spectroscopy investigation of plasma-modified polystyrene surfaces. J Vac Sci Technol A 19:856–860

    Article  CAS  Google Scholar 

  • Wong KS, Hung YM, Tan MK (2021) Hybrid treatment via MHz acoustic waves and plasma to enhance seed germination in mung bean. IEEE Trans Ultrason Ferroelectr Freq Control 68:3438–3445

    Article  PubMed  Google Scholar 

  • Xing JJ, Cheng YL, Chen P, Shan L, Ruan R, Li D, Wang LJ (2019) Effect of high-pressure homogenization on the extraction of sulforaphane from broccoli (Brassica oleracea) seeds. Powder Technol 358:103–109

    Article  CAS  Google Scholar 

  • Xu YY, Tian Y, Ma RN, Liu QH, Zhang J (2016) Effect of plasma activated water on the postharvest quality of button mushrooms, Agaricus bisporus. Food Chem 197:436–444

    Article  CAS  PubMed  Google Scholar 

  • Zhang D, Liu YB, Li XD, Xiao JX, Sun JX, Guo LP (2022) Inactivation of Escherichia coli on broccoli sprouts via plasma activated water and its effects on quality attributes. LWT 154:112761

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 31801457) and the National Collage Students Innovation Program of China (No. 202210435033).

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  1. Ting Jiang shares an equal contribution as the first author.

Authors and Affiliations

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Gongheng Che, Ting Jiang, Xiaodan Li, Junxia Xiao, Liang Liu, Jianteng Wei & Liping Guo

  2. Key Laboratory of Special Food Processing (Co-Construction By Ministry and Province), Ministry of Agriculture Rural Affairs, Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Xiaodan Li, Junxia Xiao, Liang Liu, Jianteng Wei & Liping Guo

  3. Shandong Technology Innovation Center of Special Food, Qingdao, 266109, Shandong, China

    Xiaodan Li, Junxia Xiao, Liang Liu, Jianteng Wei & Liping Guo

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  1. Gongheng Che
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Contributions

G. C.: Investigation, data curation, writing-original draft; J. T.: data curation, grammar check; D. L.: formal analysis, Software; J. X.: software, Project administration; L. L: methodology, writing-review & editing; L. G.: funding acquisition, writing-review & editing, validation.

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Correspondence to Liping Guo.

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Handling Author: Boon Chin Tan.

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Che, G., Jiang, T., Li, X. et al. Effect of plasma activated water Immersion on Broccoli Seed Germination and Nutritional Quality of Sprouts. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11272-4

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