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Fabrication of folic acid-embedded aminated drug encapsulated zeolitic imidazolate framework as promising drug delivery system for lung cancer

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Abstract

A novel pH-responsive drug carrier for the delivery of specific Docetaxel (DTX) administration is developed based on a zeolitic imidazolate framework (ZIF-8). Aminating the surface of ZIF-8 is allowed for the conjugation of folic acid (FA). Several spectroscopic studies characterized the newly fabricated DTX-encapsulated folic acid-embedded ethylene diamine (ED) ZIF-8 nanocomposites (DTX/FA@ED-ZIF-8). It has excellent chemical stability and high drug-loading efficiency. DTX from the folic acid-embedded aminated ZIF-8 (FA@ED-ZIF-8) is three-fold more efficient under acidic pH (5.0) than in physiological settings (pH 7.4), according to in vitro drug release tests. DTX/FA@ED-ZIF-8 exhibited cytotoxicity of 76.8% in an MTT experiment conducted in A549 and H1299 cells. Cell morphological and nuclear staining were investigated in the fabricated samples to support the MTT experiments. Further, the apoptosis mode of cell death was examined using Annexin V-FITC and PI by flow cytometry. These findings indicate that FA@ED-ZIF-8 holds great potential as a drug carrier for precise dosing.

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Funding

This project was supported by Researchers Supporting Project number (RSP2024R383), King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, King Saud University, PO Box 2455, 11451, Riyadh, Saudi Arabia

    Arunachalam Chinnathambi & Sulaiman Ali Alharbi

  2. Department of Pharmacology and Toxicology, School of Pharmacy, University of Connecticut, Storrs, CT, 06268, USA

    Mohankumar Ramar

  3. Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, 602 105, India

    Palanisamy Arulselvan

  4. Centre for Cancer Research, Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641 021, India

    Sridhar Muthusami

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  1. Arunachalam Chinnathambi
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Contributions

The authors confirm their contribution to the paper as follows: Arunachalam Chinnathambi and Sulaiman Ali Alharbi: Conceptualization, Writing-Reviewing, and Editing. Sridhar Muthusami: Software, Data curation, Investigation, Formal analysis, Software, Validation. Mohankumar Ramar: Visualization, Methodology. Palanisamy Arulselvan: Supervision. All authors reviewed the results and approved the final version of the manuscript.

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Chinnathambi, A., Alharbi, S.A., Ramar, M. et al. Fabrication of folic acid-embedded aminated drug encapsulated zeolitic imidazolate framework as promising drug delivery system for lung cancer. Journal of Materials Research (2024). https://doi.org/10.1557/s43578-024-01328-2

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