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Transcriptomic Analysis of Cyclamen persicum to Identify Invovled Genes in Triterpene Secondary Metabolites Pathway

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Abstract

Saponins are considered as a diverse group of natural active compounds, which are widely found in crops. Mevalonate pathway (MVA) is regarded as the main pathway for synthesis of saponins in crops. This study aims to compare transcriptome of the leaf with tuber of crop including tubers and roots. First, more than 166 million reads were generated. The existence of 36,678 unigenes in the two samples out of 48,936 assembled ones showed a significant difference in expression. Finally, 310 and 290 highly up-regulated genes in leaf and tuber were selected for the next analysis. In addition, the expression profiles of 13 key genes in the MVA pathway were compared in RNA sequencing and reverse transcription-quantitative polymerase chain reaction analysis. The results indicated that cyclamen tuber has a higher level of expression of MVA pathway genes. The topological analysis for gene co-expression network involved in triterpenoid synthesis represented that the genes at the beginning of such pathway play a critical role so that the reduction of their expression challenges triterpenoid synthesis severely. The tuber of the cyclamen appears to be the major site of triterpene synthesis, and transfer of excess Isopentenyl pyrophosphate (IPP) from tuber to leaf activates downstream genes in leaf of crop.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon request.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, University of Zabol, Daneshgah Blvd., Zabol, Iran

    Zahra Hekmati, Mahmood Solouki & Abbasali Emamjomeh

  2. Laboratory of Computational Biotechnology and Bioinformatics (CBB Lab), Department of Bioinformatics, University of Zabol, Zabol, Iran

    Abbasali Emamjomeh

  3. Department of Neuroscience, University of California, San Diego, San Diego, CA, USA

    Javad Zahiri

  4. Department of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

    Asghar Mirzaie-Asl

Authors
  1. Zahra Hekmati
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  2. Mahmood Solouki
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  3. Abbasali Emamjomeh
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  4. Javad Zahiri
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  5. Asghar Mirzaie-Asl
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Contributions

AA, AM and MS conceived the idea of this Focus paper. AA and AM conceived and designed this project. ZH performed the experimental steps. AM and JZ analyzed the data. MS provide suggestions for modification. ZH and AM wrote the paper. AA and MS revised this paper. All authors read and approved this manuscript.

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Correspondence to Mahmood Solouki or Abbasali Emamjomeh.

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Hekmati, Z., Solouki, M., Emamjomeh, A. et al. Transcriptomic Analysis of Cyclamen persicum to Identify Invovled Genes in Triterpene Secondary Metabolites Pathway. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10745-1

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