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Protective effect of resveratrol on nickel-refining fumes-induced inflammatory damage

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Abstract

Nickel (Ni), a ductile and hard silver-white transition metal, is commonly found in occupational environments and can harm the human body. Since it is a toxic compound, long-term Ni exposure can cause pneumonia, rhinitis, and other types of respiratory inflammatory diseases. Resveratrol (Res) is a plant antitoxin polyphenol, which also has anti-cancer and anti-inflammatory properties. In this report, the toxicity of Ni-refining fumes on the human lung bronchial epithelial (BEAS-2B) cells, as well as the protective effects of Res were investigated in vitro, and the specific mechanism of its anti-inflammatory effect was explained. The experimental observations of this study revealed that Ni-refining fumes induce BEAS-2B cell damage, increase reactive oxygen species (ROS) content, activate NLRP3 (LRR-, NOD-, and pyrin domain-containing 3) inflammasome, and promote the secretion of the cytokine Interleukin (IL)-1β, leading to cellular inflammation and reducing cell activity. Resveratrol (20 μmol/L) activated sirtuin 1 (SIRT1) in BEAS-2B cells to increase protein and mRNA expression. SIRT1 was observed to inhibit the transcriptional activity of nuclear factor-kappaB (NF-κB), reduced the expression of NLRP3 protein and mRNA, and inhibited NLRP3 inflammation. The level of inflammasome activation and IL-1β overexpression could reduce the inflammatory damage caused by the Ni-refining fume particles on the BEAS-2B cells and exert anti-inflammatory protective effects. In vivo experiments further confirmed that resveratrol could effectively alleviate the acute inflammatory injuries caused due to exposure to the Ni-refining fume particles in the lung tissues of the Wistar rats, and verified that resveratrol could exert its anti-inflammatory impact through the SIRT1-NF-κB-NLRP3 pathway. These results provide an important theoretical basis for developing novel protective drugs and investigating the mechanism of action for inflammatory injury in occupational populations caused by exposure to nickel and other heavy metals.

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Funding

This study was supported partly by grants from the National Natural Science Foundation of China (82273601).

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Authors and Affiliations

  1. Department of Occupational Health, School of Public Health, Harbin Medical University, 157 Baojian Road, Harbin, 150086, Heilongjiang Province, People’s Republic of China

    Yu-Lin Pan, Rui-Ze Wu, Yao Fu & Yong-Hui Wu

  2. School of Medicine and Health, Harbin Institute of Technology, Harbin, 150001, Heilongjiang Province, People’s Republic of China

    Rui Xin

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Contributions

YLP, RX, and YHW designed the study; YF conducted the study; RX performed the statistical analyses and interpreted the data; RZW contributed to study materials and consumables; RX, YLP and RZW wrote the manuscript; PYL and RZW contributed equally to this work.

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Correspondence to Rui Xin or Yong-Hui Wu.

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All experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) guidelines at Harbin Medical University.

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Pan, YL., Wu, RZ., Fu, Y. et al. Protective effect of resveratrol on nickel-refining fumes-induced inflammatory damage. Cell Biochem Biophys (2024). https://doi.org/10.1007/s12013-024-01263-3

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