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Predictive Value and Immunological Role of the HSPA5 Gene in Cervical Cancer

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Abstract

Cervical cancer (CC) ranks fourth among women’s malignancies worldwide and seriously affects women’s health. HSPA5 is a heat shock protein, also known as glucose regulatory protein 78 (GRP78). Upregulation of HSPA5 has been reported to be closely associated with multiple types of tumors. However, the specific role of HSPA5 in cervical cancer has not been discovered. In our study, we explored the prognostic value of HSPA5 in CC. Here, we analyzed the (TCGA) and (UCSC) databases, the analysis of HSPA5 in many tumors types was conducted with the “wilcox. test” method. A False Discovery Rate (FDR) value < 0.05 and Log2 | (fold change, FC) |> 1 were set as the cutoffs. “*”, “**”, and “***” indicate FDR < 0.05, < 0.01, and < 0.001, respectively, and further used human cervical cancer cells for q-PCR and western blotting detection. q-PCR and western blotting results showed that HSPA5 was highly expressed in cervical cancer cells, while it was expressed at low levels in normal cells (P < 0.05).We also analyzed the immunohistochemical data. immunohistochemical analysis results showed that HSPA5 was highly expressed in human cervical cancer, while it was expressed at low levels in normal tissues (P < 0.05). Analysis in TCGA-UCSC showed that the proportion of G3 in the group with high expression of HSPA5 was relatively high (P < 0.05). Enrichment analysis and survival analysis showed that the increased expression of HSPA5 in cervical cancer was related to the survival of CC and was involved in the regulation of biological behavior and molecular signaling pathways of cervical cancer. The correlation analysis of immune checkpoint and immune infiltration showed that HSPA5 was involved in the regulation of immune process of cervical cancer (P < 0.05). Drug sensitivity correlation analysis showed that HSPA5 was a sensitive target for tumor drugs (P < 0.05). In brief, those results suggest that HSPA5 can act as an oncogene of CC development and can serve as an effective predictive biomarker in cervical cancer.

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Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.

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Acknowledgements

Thanks to Professor Feng jiang for editor the manuscript, Thanks to wordvice company for grammar and sentence modification and polishing.

Funding

This study was supported by grants from Construction of Gansu Provincial Administration of Traditional Chinese Medicine (Fund number: GZKG-2020-46).

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Author notes

  1. Yingying Bai, Yandong Miao and Jiangtao Wang have contributed equally to this work.

Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Tangdu Hospital, Air Force Medical University, 569Xinsi Road, Baqiao District, Xian, 710038, Shanxi, China

    Yingying Bai & Jiang Feng

  2. Cancer Center, Yantai Affiliated Hospital of Binzhou Medical University, The 2nd Medical College of Binzhou Medical University, Yantai, 264100, China

    Yandong Miao

  3. Department of General Surgery, Yantai Affiliated Hospital of Binzhou Medical University, The Second Clinical Medical College of Binzhou Medical University, Yantai, 264000, China

    Jiangtao Wang & Jian Gan

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  1. Yingying Bai
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  3. Jiangtao Wang
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  4. Jian Gan
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  5. Jiang Feng
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Contributions

Conceptualization: Fengjiang, Yingying Bai. Data curation: Yandong Miao, Jiangtao Wang, Jian Gan. Methodology: Yandong Miao, Jiangtao Wang, Jian Gan. Writing-original draft: Yingying Bai, Yandong Miao. Writing-review editing: Fengjiang, Yingying Bai, Yandong Miao. Project: Fengjiang. All authors reviewed the manuscript. All authors contribute equally.

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Correspondence to Jiang Feng.

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Bai, Y., Miao, Y., Wang, J. et al. Predictive Value and Immunological Role of the HSPA5 Gene in Cervical Cancer. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10782-w

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