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Proteinase Resistance of Carnosine, Pyrrolylcarnosine and Salicylcarnosine

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Abstract

Objective: The resistance of carnosine, pyrrolylcarnosine (PC) and salicylcarnosine (SC) to the action of leucine aminopeptidase and carboxypeptidases B and Y was evaluated. Methods: Proteolysis of carnosine and its derivatives under the action of leucine aminopeptidase, carboxypeptidases Y and B, or under the action of enzyme systems of plasma membranes of rat brain cells or blood plasma. Results and Discussion: It was found that proteolysis of carnosine, PC and SC under the action of leucine aminopeptidase does not occur. Carboxypeptidases B and Y, as well as the enzyme systems of blood plasma and plasma membranes of rat brain cells, degraded peptides containing β-alanyl, N-pyrrolyl, N-salicylic fragments to varying degrees. In all cases, histidine was formed. The formation of pyrrole or salicylic acid did not occur. Conclusions: It was found that carnosine, PC and SC showed high resistance to the action of amino- and carboxypeptidases in in vitro experiments.

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DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was done within the framework of the state assignment according to the plan of research development plan of the National Research Centre “Kurchatov Institute” and withing the framework of the topic no. 1021052806528-6-1.6.4;3.1.1;3.1.9;3.1.8;3.2.25 “Fundamental aspects of neuroplasticity within the framework of translational neurology model” of the Research Center of Neurology.

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Authors and Affiliations

  1. National Research Centre “Kurchatov Institute” (NRC “Kurchatov Institute”), 123182, Moscow, Russia

    K. V. Shevchenko, I. Yu. Nagaev, V. P. Shevchenko & N. F. Myasoedov

  2. Research Center of Neurology (RCN), 125367, Moscow, Russia

    O. I. Kulikova & S. L. Stvolinsky

Authors
  1. K. V. Shevchenko
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  2. I. Yu. Nagaev
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  3. O. I. Kulikova
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  4. S. L. Stvolinsky
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  5. V. P. Shevchenko
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  6. N. F. Myasoedov
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Contributions

All authors made equal contributions to the writing of the article.

Corresponding author

Correspondence to K. V. Shevchenko.

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All applicable international, national and/or institutional guidelines for animal care were followed.

Experimental procedures for obtaining tissues from rats were approved by the local ethics committee of the Society for Experimental Animal Care (National Research Centre “Kurchatov Institute”): protocol no. 708n, August 23, 2010. No conflict of interest was declared by the authors.

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Abbreviations: PC, pyrrolylcarnosine; SC, salicylcarnosine; HCD, histidine-containing dipeptides; RBMF, rat brain microsomal fraction; PMSF, phenylmethylsylfonyl fluoride.

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Shevchenko, K.V., Nagaev, I.Y., Kulikova, O.I. et al. Proteinase Resistance of Carnosine, Pyrrolylcarnosine and Salicylcarnosine. Russ J Bioorg Chem 50, 401–407 (2024). https://doi.org/10.1134/S1068162024020201

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