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The Long Noncoding RNA Gall Bladder Cancer-Associated Suppressor of Pyruvate Carboxylase Inhibits the Proliferation, Migration, and Invasion of Colorectal Cancer Cells and Induces Their Apoptosis

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Abstract

This study aimed to determine the role of the long noncoding RNA (lncRNA) gall bladder cancer-associated suppressor of pyruvate carboxylase (SOD2-1) in the progression of colorectal cancer (CRC). A total of 23 pairs of specimens, including CRC tissues and adjacent normal tissues, were collected, and the expression of lncRNA SOD2-1 (lnc-SOD2-1) was measured. lnc-SOD2-1 function was examined using HCT15 and HCT116 cells. A lnc-SOD2-1 overexpression vector was designed and transfected into both cell lines. MTS and colony formation assays were used to determine cell viability. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling assays were performed to measure apoptosis. Cell migration and invasion were evaluated using the Transwell assay. Migration and invasion markers were validated using quantitative reverse transcription–polymerase chain reaction and western blot analysis. The results indicated that the expression of lnc-SOD2-1 was downregulated in CRC tissues. lnc-SOD2-1 overexpression evidently decreased cell viability and led to the formation of fewer cell colonies. lnc-SOD2-1 overexpression induced ~ twofold higher apoptosis than the control group. lnc-SOD2-1 overexpression reduced the proportion of migratory and invasive cells to 50% and 75% of the control group, respectively. lnc-SOD2-1 overexpression significantly decreased the expression of matrix metalloproteinase-2 and -9. In conclusion, lnc-SOD2-1 may act as a tumor suppressor that inhibits the proliferation, migration, and invasion of CRC cells and induces their apoptosis.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Author notes

  1. Tingting Xie and Jianian Guo have contributed equally to this work.

Authors and Affiliations

  1. Department of Gastroenterology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

    Tingting Xie & Jianian Guo

  2. Department of Thoracic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, No.250 Changgang East Road, Haizhu District, Guangzhou, 510260, China

    Wei Wang

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  1. Tingting Xie
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  2. Jianian Guo
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Contributions

Tingting Xie and Jianian Guo conceived and designed the study and drafted the manuscript. Tingting Xie, Jianian Guo, and Wei Wang performed the experiments and analyzed and interpreted the data. Wei Wang critically revised the manuscript and prepared the figures.

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Correspondence to Wei Wang.

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All procedures were performed in accordance with the Declaration of Helsinki. Moreover, this study was approved by the Ethics Committee of the Second Affiliated Hospital of Guangzhou Medical University (Approval number: 2021-KY-ks-02).

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Xie, T., Guo, J. & Wang, W. The Long Noncoding RNA Gall Bladder Cancer-Associated Suppressor of Pyruvate Carboxylase Inhibits the Proliferation, Migration, and Invasion of Colorectal Cancer Cells and Induces Their Apoptosis. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10786-6

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